LOCUS       sequence-361776-        4385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet fusion cloning vector for mosTI(unc-119). Transgene 
            inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate 
            (BsaI: tgcc - MCS - aaaa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2
FEATURES             Location/Qualifiers
     source          1..4385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(642..675)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      1609..1932
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2291..2307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2291..2307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2304..2326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2479..3067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2559..2578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3184..3208)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3308..4099,4100..4168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3931..3950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4169..4273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4372..4385,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
       61 cgcctgacct aagtgctttt cgagatcgcc aaaccggaga acgatcagga gaatgatgag
      121 tcgccacagg agtcggcaag atacgtgcgt tatagatttg ctccaaactt tttgaaactc
      181 aaaacggtcg gagcaactgt ggaattcaaa gtaggagaca tcccaatcca tcatttccga
      241 atgatcgaac gtcacttctt caaagatcgc cttctgaagt gttttgactt tgaattcgga
      301 ttctgtattc cgaattcacg aaacaactgt gaacatatct atgagttccc tcaactctct
      361 caacaactca gtgagtcatt attctaaaaa gtacaattca aaagactaat ctctttttca
      421 gtggacgaca tgatcaacaa tccaaacgag actcgttctg acagcttcta tttcgtcgat
      481 aacaaactcg tcatgcacaa caaagccgac tactcatatg atgcataaat cttcattgtt
      541 gagtttatct tgttgatttt tgaataaatt atcaactctt tactttttaa tgggttatga
      601 aataaataaa cattgaaaac tgataaacaa cgttcatctc tataacttcg tatagcatac
      661 attatacgaa gttattgcct gagaccagct agcaagcttg catgcctgca ggtcgactct
      721 agaggatccc cgggtaccga gctcaggtct ctaaaaatgg tttctaaggg cgaagccgtc
      781 atcaaggtaa gttttacgaa attctgaagc tcacaaaaca tggagtacat ttttcaggag
      841 ttcatgcggt tcaaggtcca catggagggt agtatgaacg gacacgagtt cgagatagag
      901 ggagagggag agggacggcc atacgaggga acccaaacgg ccaagctcaa ggtcaccaaa
      961 gggggaccac tcccattctc ctgggatatc ctctccccac agttcatgta cggaagtcgt
     1021 gccttcacca agcaccccgc aggtaagttt ataatctatg atcttaattt ggcaatggaa
     1081 aaagtttttt cagatatacc agattactat aagcagtcct tcccagaggg attcaaatgg
     1141 gagcgtgtca tgaacttcga ggatggagga gccgtcaccg tcacgcaaga cacgtccctc
     1201 gaggatggaa ccctaatcta caaggtaaag cttcgtggaa ccaacttccc cccaggtaag
     1261 ttttattcca taatacaaat atatattgaa caataatgct tttcagatgg accagtcatg
     1321 caaaagaaaa ctatgggatg ggaagcctcg accgaacgtc tctacccaga ggacggggtc
     1381 ctcaaaggag atatcaagat ggccctaaga ctcaaggacg gaggacggta tcttgccgat
     1441 ttcaagacca cgtacaaggc taagaagcca gtacaaatgc caggggccta caacgtagac
     1501 cgtaaactcg acatcacgtc ccacaacgag gactacacgg tcgtcgaaca atacgaacgt
     1561 tccgaaggac gtcactccac cggagggatg gacgagctct acaagtaaat gcaaaatcct
     1621 ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct
     1681 aatttatttg cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg
     1741 catctgagtg aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc
     1801 tcttcaattc cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca
     1861 aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc
     1921 aagaaaaaag tctcacagga aacggagaat ctgtgcggca atgtcttatc atttgaaacg
     1981 cgccgtatcg atgctgacat catgcggctc ccgtgttagt cgagaagatg ggtacgggcc
     2041 tttattcatc caacactcta cggcttcttc aagagctagt agggcaccct gtagttggaa
     2101 agggattatt tcgtatggcg agcccatacc gtctttcttg cggaagctta acacgatagg
     2161 aagttggata gtttcgaacg atggttccat acggaacgct gtctcaagga tgagtctgac
     2221 ggagtgtaac tcgaaggcta ggtggaggct cagtgatgat aagtctgcga tggtggatgc
     2281 atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga gggcacaatc
     2341 ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc ggcgtatggc
     2401 atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2641 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2881 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca acaaagccgc
     3121 cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtcctcgt gataccaatt
     3181 cggagcctgc ttttttgtac aaacttgttg ataatggcaa ttcaaggatc ttcacctaga
     3241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3301 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3361 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3421 ctggccccag tgctgcaatg ataccgcgag agccacgctc accggctcca gatttatcag
     3481 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3541 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3601 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3661 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3721 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3781 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3841 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3901 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3961 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4021 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4081 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4141 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4201 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4261 taggggttcc gcgcacattt ccccgaaaag tgccagatac ctgaaacaaa acccatcgta
     4321 cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac
     4381 gacgt
//
