LOCUS       sequence-361778-        2522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 
            genomic locus. Use with pSEM371 fragment included in Ex array..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM376 - sgRNA - spc4 - array - insertion - u...
FEATURES             Location/Qualifiers
     source          1..2522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..120
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     112..129
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     113..129
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(539..555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(539..555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(552..574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(727..1315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(807..826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(1432..1456)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1556..2347,2348..2416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASQQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2179..2198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2417..2521)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
       61 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg
      121 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc
      181 tcgcctcccc gcacactcaa cacagcgagc ggcggaaccc gcggggtgtc ggccgctcgg
      241 acacgctccc gtatataacg acgcggcgcg cgtcaagttg tgtttgggaa ccaggtgttg
      301 ggtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
      361 cttgaaaaag tggcaccgag tcggtgcttt ttgttttttg cccatttttt gtagtttgag
      421 actggaaaaa tcgtaaatca accatagttt tagggcaaaa ttctcgaaat ttttctaaaa
      481 ataggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt
      541 catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct attccgcgct
      601 atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg
      661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1321 tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg tcccgtcaag
     1381 tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga taccaattcg gagcctgctt
     1441 ttttgtacaa acttgttgat aatggcaatt caaggatctt cacctagatc cttttaaatt
     1501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1681 ctgcaatgat accgcgagag ccacgctcac cggctccaga tttatcagca ataaaccagc
     1741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1801 ttaattgttg ctgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2521 gc
//
