LOCUS       sequence-361779-        5028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous 
            nuclear GFP expression after mosTI insertion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
FEATURES             Location/Qualifiers
     source          1..5028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..27
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(660..693)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        694..1293
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             1309..1329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2270..2593
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2952..2968)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2952..2968)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2965..2987)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3140..3728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3220..3239)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3845..3869)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3969..4760,4761..4829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4592..4611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4830..4934)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttttcccagt cacgacgttg taaaacgacg gccagtcatg cataatccgc acgcatctgg
       61 aataaggaag tgccattccg cctgacctaa gtgcttttcg agatcgccaa accggagaac
      121 gatcaggaga atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct
      181 ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc
      241 ccaatccatc atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt
      301 tttgactttg aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat
      361 gagttccctc aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa
      421 agactaatct ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac
      481 agcttctatt tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat
      541 gcataaatct tcattgttga gtttatcttg ttgatttttg aataaattat caactcttta
      601 ctttttaatg ggttatgaaa taaataaaca ttgaaaactg ataaacaacg ttcatctcta
      661 taacttcgta tagcatacat tatacgaagt tatgcacctt tggtctttta ttgtcaactt
      721 ccattggttc ttccattgtt tctgttaaat taatgaattt ttcataaaat aaagacatta
      781 tacaatataa aaatgaagaa tttattgaaa ataaactgcc agagagaaaa agtatgcaac
      841 actcccgccg agagtgtttg aaatggtgta cggtacattt tcgtgctagg agttagatgt
      901 gcaggcagca acgagagggg gagagatttt tttgggcctt gtgaaattaa cgtgagtttt
      961 ctggtcatct gactaatcat gttggttttt tgttggttta ttttgttttt atctttgttt
     1021 ttatccagat taggaaattt aaattttatg aatttataat gaggtcaaac attcagtccc
     1081 agcgtttttc ctgttctcac tgtttagtcg aatttttatt ttaggctttc aacaaatgtt
     1141 ctaactgtct tatttgtgac ctcacttttt atattttttt aatttttaaa aatattagaa
     1201 gtttctagga taattttttc gacttttatt ctctctaccg tccgcactct tcttactttt
     1261 aaattaaatt gttttttttt cagttgggaa acactttgcc taaaaatgcc aaagaagaag
     1321 agaaaggtgt caaaaggaga agaactcttt accggagtcg tcccaattct cgtcgagctc
     1381 gacggagacg taaacggaca caaattctcg gtttccggag aaggagaagg aggtaagttt
     1441 tacgaaattc tgaagctcac aaaacatgga gtacattttt cagatgctac ttatggaaaa
     1501 ctcaccctta aattcatttg caccaccgga aagctcccag tgccgtggcc gacccttgtg
     1561 actacattct gctacggagt tcaatgcttt tcccgctacc cagaccacat gaagcgtcac
     1621 gatttcttca aatcagccat gccagaagga tacgtccagg agcgaacaat tttcttcaag
     1681 gacgatggaa actacaagac tcgtgctgaa gtcaagtttg aaggagatac tctcgtgaac
     1741 cgcattgagc tcaaggtaag tttataatct atgatcttaa tttggcaatg gaaaaagttt
     1801 tttcagggaa tcgacttcaa agaagatgga aacattcttg gacacaagct cgaatacaac
     1861 tataactcgc acaacgtgta tatcatggcc gacaagcaaa aaaatggaat caaggtcaac
     1921 ttcaagattc gccacaacat cgaagacggg tcggttcaac tcgctgatca ctaccagcag
     1981 aacacaccaa ttggaggtaa gttttattcc ataatacaaa tatatattga acaataatgc
     2041 ttttcagatg gaccagtcct cctccctgat aatcactacc tttccactca atccgctctt
     2101 agcaaggatc caaatgagaa aagagatcac atggttcttc tcgagtttgt caccgccgcc
     2161 ggaatcaccc acggaatgga cgagctttac aagtcgagac gccgcaaggc taatccaacc
     2221 aaactttctg aaaatgccaa gaaacttgcc aaggaggtcg agaactgaga tgcaaaatcc
     2281 tttcaagcat tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa attctctcgc
     2341 taatttattt gcttttttaa tgttattatt ttatgacttt ttatagtcac tgaaaagttt
     2401 gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt ctgatgtact ttcacaatct
     2461 ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt tgagaaaaat gcgagcgctc
     2521 aaatatttgt attgtgttcg ttgagtgacc caacaaaaag aggaaacttt attgtgccgc
     2581 caagaaaaaa gtctcacagg aaacggagaa tctgtgcggc aatgtcttat catttgaaac
     2641 gcgccgtatc gatgctgaca tcatgcggct cccgtgttag tcgagaagat gggtacgggc
     2701 ctttattcat ccaacactct acggcttctt caagagctag tagggcaccc tgtagttgga
     2761 aagggattat ttcgtatggc gagcccatac cgtctttctt gcggaagctt aacacgatag
     2821 gaagttggat agtttcgaac gatggttcca tacggaacgc tgtctcaagg atgagtctga
     2881 cggagtgtaa ctcgaaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg
     2941 catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat
     3001 cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg
     3061 catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3121 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3181 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3241 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3301 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3361 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3421 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3481 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3541 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3601 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3661 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3721 gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg
     3781 ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtcctcg tgataccaat
     3841 tcggagcctg cttttttgta caaacttgtt gataatggca attcaaggat cttcacctag
     3901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4081 tctggcccca gtgctgcaat gataccgcga gagccacgct caccggctcc agatttatca
     4141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4261 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4561 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4861 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4921 ataggggttc cgcgcacatt tccccgaaaa gtgccagata cctgaaacaa aacccatcgt
     4981 acggccaagg aagtctccaa taactgtgat ccaccacaag cgccaggg
//
