LOCUS       sequence-361783-       16497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila integration plasmid expressing Bcd-LEXY fusion protein 
            under the maternal tubulin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Singh AP, Wu P, Ryabichko S, Raimundo J, Swan M, Wieschaus E, Gregor
            T, Toettcher JE
  TITLE     Optogenetic control of the Bicoid morphogen reveals fast and slow 
            modes of gap gene regulation.
  JOURNAL   Cell Rep. 2022 Mar 22;38(12):110543. doi: 
            10.1016/j.celrep.2022.110543.
  PUBMED    35320726
REFERENCE   2  (bases 1 to 16497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-03-22"; volume: "38(12):110543. doi"; pages: " 
            10.1016/j.celrep.2022.110543"
COMMENT     Sequence Label: pBABR Bcd-LEXY
FEATURES             Location/Qualifiers
     source          1..16497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2446..2535
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    4495..4542
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    7277..7324
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    8555..8624
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(8788..8989,8990..9020)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(9558..9577)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9677..9699
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9737..9755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9823..9927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9928..9996,9997..10788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10146..10165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10959..11547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11448..11467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(11782..11812,11813..12367)
                     /label=P element 5' end
     CDS             complement(join(13146..13760,13831..13962,14166..14481,
                     14543..15197,15272..15545,15947..16018))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     16470..16488
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 aattggccgc tctagccccc cctcgaatgt tctctctctt ctcttctctc tctctttctc
       61 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctaag
      121 ggtcgagtcg atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt
      181 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaaccggtcc acggccgctt
      241 ggagggagat tagcaaaacg cagcgcgtga tcaaggtgac aatgaagcca ccgaccacca
      301 cggtggcgcc caacaaggca cgagtggttt gcatgtccga cacgcactcc ctgacgccct
      361 acatcaagtt cgatatacca gatggcgata tattcataca tgccggcgat tttaccaagt
      421 gcggccaact ggaggaggtg gaggagttta acacctggat tggagcactg ccccatcgcc
      481 acaagatcgt gattgctggc aatcatgagc tcagcttcga tagaaccttc acccatcctt
      541 tccagaagag caaaagtcat gcttcgagca gcaaacacac cggcatgtcc atcctcgatg
      601 atctgcccac tttgggcaac gccaaggaga acttggagag cgctgtgcaa acgcagaatg
      661 tccgcgatgt tctgaccaac tgtcggtatt tggaggatga actacttgag atttggggta
      721 ttcaaatata tggctcgcca tggcaaccgg aattctgtcg ctgggccttt aatgttccac
      781 gtggaactgc ctgtttggat aagtggaatc aaatcccgga aggcatcgat atcctggtca
      841 cccacacgcc gcccgtgggt cacggcgatc tctgctgttc cggcgtacga gccggttgtg
      901 tggagctact cagcacagtg cagcaacgag tgcgcccgaa ataccatgtc ttcggacatg
      961 tacacgaagg ctacggcatc accagcgatg gcaggatcat ctttgtgaat gcctcaacgt
     1021 gtgacatcaa ctacctaccc aacaatgccc ccatcgtttt cgatgtgacg ctgccccccg
     1081 gatttcgcaa ggattagcat gcctagatga ttatttattg tgtcacaggt tcaatgaatt
     1141 tattttcgtc ccactttatg tttatgttaa ggtatttcgt ctgtaaagat tttgaagcac
     1201 aattgtaggt gtctagagag atagaattca attcgcacga aattaaaaaa agaaattcac
     1261 acttaagatg ccataaaata tttattcaag tagagtgcat ttgtcaatat atgaatttgt
     1321 aaaaataaac aagttttgaa gacacctaaa cttttgaaga ttttcaatat gatattatat
     1381 agtttttaat aataatttaa gtaaaggtaa agcaaatcct gagatacata catactattt
     1441 caaattagta tcgataagcc gccagaaatg ggttggaaaa accttggaat gattattggt
     1501 tagcagccct gtctcccaat ttgacattcc tctttggcgc tctgtaacgg cgcttttcca
     1561 gtgactcccc aatcagcgaa ccaactaacc tgaggctgca cttttcgcgt ggcgaccaaa
     1621 aaaattataa aaaaaaggtc ccgcattaga ttttaatttg tatacaccca attttagtcc
     1681 agcaaaaaaa aaaactaatc aattggtgag aagaagaaaa actaattagt ttaagaattg
     1741 ctgaaagcgg gaagtgaaat ttaaaatcag aaaagatgtg taagaagatg tgaaatgaaa
     1801 aggtgtggga agtgaacatg aataaatgtt cattgaaaat gaacagctaa ttgcgaattt
     1861 tttgaaactt aactgttaac tgttactgct cacaaatcct tgaaaaatat taagatcgat
     1921 cttagaattg tttatttaaa atgaattcct cgtatgtatt ataacagaca atcaagttta
     1981 ttaaagaata tttaaacttg tatttttttt tgagtttgat aaaatgtccc aaatataaaa
     2041 gagtattacc ttttcgtcat ataatactgt tcttaatatt agttttagtt agtaatgtgt
     2101 ttataaagta aattacaaag ggaggaccta acctaacctt aaacaatggc gccttctctt
     2161 gcccagctca tcacttttat tgtttcaccc gccagcgcga agtagtgtcc atccagattg
     2221 ggacggtacc atctcttcgc tcatccctaa ataacggcac tctgcagatg cgaagcagtg
     2281 gatcgcaaaa acgcaaaatg tgggcgaaat aagttcgcga gcgtctcgaa agtatccggt
     2341 tactgaaaat acaagaaagt ttccacactc ctttgccatt tttccgcgcg gcgcttggaa
     2401 attcgtaaag ataacgcggc ggagtgtttg gggctagcaa agatgtaccc atacgatgtt
     2461 cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac
     2521 gttccagatt acgctgcggg aggtagtagt gcgcaaccgc cgccagatca aaacttttac
     2581 catcatccgc tgccccacac gcacacacat ccgcatccgc actcccatcc gcatccgcac
     2641 tcgcatccgc acccacatca ccaacatccg cagcttcagt tgccgccaca attccgaaat
     2701 cccttcgatt tgcttttcga tgagcgaacg ggagcgataa actacaacta catacgtccg
     2761 tatctgccca accagatgcc caagccagag gagctgcccg actctctggt gatgcggcga
     2821 ccacgtcgca cccgcaccac ttttaccagc tctcaaatag cagagctgga gcagcacttt
     2881 ctgcagggac gatacctcac agccccccga cttgcggatc tgtcagcgaa actagccctg
     2941 ggcacagccc aggtgaagat atggtttaag aaccgtcggc gtcgtcacaa gatccaatcg
     3001 gatcagcaca aggaccagtc ctacgagggg atgcctctct cgccgggtat gaaacagagc
     3061 gatggcgatc cccccagctt gcagactctt agcttgggtg gaggggccac gcccaacgct
     3121 ttgactccgt cacccacgcc ctcaacgccc actgcacaca tgacggagca ctacagcgag
     3181 tcattcaacg cctactacaa ctacaatgga ggccacaatc acgcccaggc caatcgtcac
     3241 atgcacatgc agtatccttc cggagggggg gcaggacctg ggtcgaccaa tgtcaatggc
     3301 ggccagttct tccagcagca gcaggtccat aatcaccagc agcaactgca ccaccagggc
     3361 aaccacgtgc cgcaccagat gcagcagcag caacagcagg ctcagcagca gcaataccat
     3421 cactttgact tccagcaaaa gcaagccagc gcctgtcgcg tcctggtcaa ggacgaaccg
     3481 gaggccgact acaacttcaa cagctcgtac tacatgcgat cgggaatgtc tggcgccact
     3541 gcatcggcat ccgctgtggc ccgaggcgct gcctcgccgg gctccgaggt ctacgagcca
     3601 ttaacaccca agaatgacga aagtccgagt ctgtgtggca tcggcatcgg cggaccttgc
     3661 gccatcgccg ttggcgagac ggaggcggcc gacgacatgg acgacggaac gagcaagaag
     3721 acgacgctac agatcttgga gcctttgaag ggtctggaca agagctgcga cgatggcagt
     3781 agcgacgaca tgagcaccgg aataagagcc ttagcaggaa ccggaaatcg tggagcggca
     3841 tttgccaaat ttggcaagcc ttcgccccca caaggccctc agccgcccct cgggatgggg
     3901 ggcgtggcca tgggcgaatc gaaccaatat caatgcacga tggatacgat aatgcaagcg
     3961 tataatcccc atcggaacgc cgcgggcaac tcgcagtttg cctactgctt caataagggt
     4021 ggatctggag gttcaggtgg aagtttggct actacacttg aacgtattga gaagaacttt
     4081 gtcattactg acccaagatt gccagataat cccattatat tcgcgtccga tagtttcttg
     4141 cagttgacag aatatagccg tgaagaaatt ttgggaagaa actgcaggtt tctacaaggt
     4201 cctgaaactg atcgcgcgac agtgagaaaa attagagatg ccatagataa ccaaacagag
     4261 gtcactgttc agctgattaa ttatacaaag agtggtaaaa agttctggaa cctctttcac
     4321 ttgcagccta tgcgagatca gaagggagat gtccagtact ttattggggt tcagttggat
     4381 ggaactgagc atgtccgaga tgctgccgag agagagggag tcatgctgat taagaaaact
     4441 gcagaaaata ttgacgagct gctgaaggag ctggccgacc tgaacctgga ctaggaagta
     4501 cctattccga agttcctatt ctctagaaag tataggaact tccctggatg agaggcgtgt
     4561 tagagagttt cattagcttt aggttaacca ctgttgttcc tgattgtaca aataccaagt
     4621 gattgtagat atctacgcgt agaaagttag gtctagtcct aagatccgtg taaatggttc
     4681 ccagggaagt tttatgtact agcctagtca gcaggccgca cggattccag tgcatatctt
     4741 agtgatactc cagttaactc tatactttcc ctgcaatacg ctattcgcct tagatgtatc
     4801 tgggtggctg ctccactgaa gcccgggaat atgcaaccag ttacatttga ggccatttgg
     4861 gcttaagcgt attccatgga aagttatcgt cccacatttc ggaaattata ttccgagcca
     4921 gcaagaaaat cttctctgtt acaatttgac atagctaaaa actgtactaa tcaaaatgaa
     4981 aaatgtttct cttgggcgta atctcataca atgattaccc ttaaagatcg aacatttaaa
     5041 caataatatt tgatatgata ttttcaattt ctatgctatg ccaaagtgtc tgacataatc
     5101 aaacatttgc gcattctttg accaagaata gtcagcaaat tgtattttca atcaatgcag
     5161 accatttgtt tcagattcgg agattttttg ctgccaaacg gaataactat catagctcac
     5221 attctaatta catcactaag aagagcattg caatctgtta ggcctcaagt ttaattttaa
     5281 aatgctgcac ctttgatgtt gtctctttaa gctttgtatt tttaattacg aaaatatata
     5341 agaactactc tactcgggta aattgtgact aactacacat aactacatac ttagcccata
     5401 tttccgtccc tttctagaat gaacgaaaac agtatctggt tttcccgaaa atcttatgaa
     5461 tttaaaaatg cactttattg cacatactca cacatgcctg ccataaaata tgattcgcga
     5521 gcggccgcgg atctaattca attagagact aattcaatta gagctaattc aattaggatc
     5581 caagcttatc gatttcgaac cctcgaccgc cggagtataa atagaggcgc ttcgtctacg
     5641 gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa
     5701 taaacaagcg cagctgaaca agctaaacaa tcgggcggcc gcactagagc cggtcgccac
     5761 catgaggtct tccaagaatg ttatcaagga gttcatgagg tttaaggttc gcatggaagg
     5821 aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc catacgaagg
     5881 ccacaatacc gtaaagctta aggtaaccaa ggggggacct ttgccatttg cttgggatat
     5941 tttgtcacca caatttcagt atggaagcaa ggtatatgtc aagcaccctg ccgacatacc
     6001 agactataaa aagctgtcat ttcctgaagg atttaaatgg gaaagggtca tgaactttga
     6061 agacggtggc gtcgttactg taacccagga ttccagtttg caggatggct gtttcatcta
     6121 caaggtcaag ttcattggcg tgaactttcc ttccgatgga cctgttatgc aaaagaagac
     6181 aatgggctgg gaagccagca ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga
     6241 gattcataag gctctgaagc tgaaagacgg tggtcattac ctagttgaat tcaaaagtat
     6301 ttacatggca aagaagcctg tgcagctacc agggtactac tatgttgact ccaaactgga
     6361 tataacaagc cacaacgaag actatacaat cgttgagcag tatgaaagaa ccgagggacg
     6421 ccaccatctg ttcctttagc ggccatcgaa ttcgagctcg cccactaagc gtcgcgccac
     6481 ttcaacgctc gatgggagcg tcattggtgg gcggggtaac cgtcgaaatc agtgtttacg
     6541 cttccaatcg caacaaaaaa ttcactgcaa cactgaaaag catacgaaaa cgatgaagat
     6601 tgtacgagaa accataaagt attttatcca caaagacacg tatagcagaa aagccaagtt
     6661 aactcggcga taagttgtgt acacaagaat aaaatcggcc agattcagtg ttgtcagaaa
     6721 taagaaaacc ccactatgtt tttctttgcc ttttctttct cccagcgatc attcatttcg
     6781 tggtgaaaga acggggtcat tgcacggagt ttcgactgcg ggaaagcaga gctgccgttc
     6841 acttcgtcta taattagcgc tttctatttt ccccgattcg ggccgctgct gcgcttttcc
     6901 gcctgctgtt tgtggcaagt gtagcagcag gctgtgcacg cagtgtggca tgcacttggc
     6961 tttccaccgt tggtatcgat tctctgggac gatgagtcat tcctttcggg gccacagcat
     7021 aatcgttgcc agctcaccga aatggtgact tcatttctta actgccgtca agcatgcgat
     7081 tgtacataca tacatattta tatatgtaca tatttatgtg actatggtag gtcgatataa
     7141 tagcaatcaa cgcaagcaaa tgtgtcagtc ctgcttacag gaacgattct atttagtaat
     7201 tttcgttgta taaagtaatt atgtatgtat gtaagcccca taaatctgaa acaattaggc
     7261 aaaaccatgc gaagctgaag tacctattcc gaagttccta ttctctagaa agtataggaa
     7321 cttccatatg gcggccgcca ttcgtagaat agggatcccc agcagtcgat tcactagcca
     7381 gctgcctctc caatcagcct gtccaaacta tccatcatga gtttcgtttt gataatttaa
     7441 tatatacaag tataaatttt ttttttttgg tttcgcgcct tctgttcttt gattcctgat
     7501 aatcctgtac ttatccgtgc atttgaatta aacaaaaagt agccaacacc agcgattgtt
     7561 atgtacgaca gtgtagtgtg tgagatgttg gtgccagata acacgatttc ccgaaaaact
     7621 cctttcataa gtattaactc attgaatata catatatatt acctatatac aaatacatac
     7681 ttatacaaga atgtggcaat aaacctttgt ttttcttaca ctcgagaacg tgcgcacgtc
     7741 caaccaaaca gatgtctgca gaaatccttc agccaaaagc aactgatcaa acaatagaaa
     7801 ctttggaata tgtaccccgc atggctaact aagcatgatc actagcatga tgtgataaaa
     7861 gtatgtagct tcaaatgtgg agggaaattg aaagagattt cggaaattag tcaaaatttt
     7921 cttggaacct tcgacaagct gactaatgag ttcatgtcca aaagactcga acttgtaaac
     7981 tcatatctta tctcacatta tttttgattc gaagatattt cagcttctgc aaatatatgt
     8041 ttttcaagaa tttatttaaa aaaaaaatca attaaataga tggcttaatg ttcgaaacac
     8101 tcaacgttgt atattacaca ctttttggac aactattaat ttaaaagaaa acacgaacat
     8161 ttgaaaatct agaactagtg gaatctggat ccagactgca gagaagatct atagaattca
     8221 gagaggccgg cagagaggct agcaggcatg catctagagt cgacctcgaa cgttaacgtt
     8281 aacgtaacgt taactcgagg agcttgataa cattatacct aaacccatgg tcaagagtaa
     8341 acatttctgc ctttgaagtt gagaacacaa ttaagcatcc cctggttaaa cctgcttgat
     8401 agtgcaggct atacagaact gcgctagtga ctcctatccg cctgcgtttc tctgcctgct
     8461 cacgggtcat atgcgtgctt tggagctgag ggtgcggcgg attggcgtcg acgatgtagg
     8521 tcacggcggg catgtcgacg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg
     8581 gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatga
     8641 acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc
     8701 cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg gcgtcgacga
     8761 tgtaggtcac ggcgggcagc caagctttgc gtactcgcaa attattaaaa ataaaacttt
     8821 aaaaataatt tcgtctaatt aatattatga gttaattcaa accccacgga catgctaagg
     8881 gttaatcaac aatcatatcg ctgtctcact cagactcaat acgacactca gaatactatt
     8941 cctttcactc gcacttattg caagcatacg ttaagtggat gtctcttgcc gacgggacca
     9001 ccttatgtta tttcatcatg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     9061 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     9121 cttgtttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     9181 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     9241 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
     9301 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
     9361 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
     9421 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
     9481 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggtgcggtat ttcacaccgc
     9541 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     9601 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     9661 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     9721 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9781 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    10021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    10081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    10141 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    10201 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    10261 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10321 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10381 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10441 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10501 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10561 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10621 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    10681 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10741 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10801 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    11041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    11101 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    11161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    11221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    11281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11341 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11521 tcgtcagggg ggcggagcct atggaaaaac gccttcttct tgaactcggg ctcggtgcca
    11581 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
    11641 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
    11701 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
    11761 ctggactacg tgggtctggc ccatgatgaa ataacataag gtggtcccgt cgatagccga
    11821 agcttaccga agtatacact taaattcagt gcacgtttgc ttgttgagag gaaaggttgt
    11881 gtgcggacga attttttttt gaaaacatta acccttacgt ggaataaaaa aaaatgaaat
    11941 attgcaaatt ttgctgcaaa gctgtgactg gagtaaaatt aattcacgtg ccgaagtgtg
    12001 ctattaagag aaaattgtgg gagcagagcc ttgggtgcag ccttggtgaa aactcccaaa
    12061 tttgtgatac ccactttaat gattcgcagt ggaaggctgc acctgcaaaa ggtcagacat
    12121 ttaaaaggag gcgactcaac gcagatgccg tacctagtaa agtgatagag cctgaaccag
    12181 aaaagataaa agaaggctat accagtggga gtacacaaac agagtaagtt tgaatagtaa
    12241 aaaaaatcat ttatgtaaac aataacgtga ctgtgcgtta ggtcctgttc attgtttaat
    12301 gaaaataaga gcttgaggga aaaaattcgt actttggagt acgaaatgcg tcgtttagag
    12361 cagcagccga attaattcta gttccagtga aatccaagca ttttctaaat taaatgtatt
    12421 cttattatta tagttgttat ttttgatata tataaacaac actattatgc ccaccatttt
    12481 tttgagatgc atctacacaa ggaacaaaca ctggatgtca ctttcagttc aaattgtaac
    12541 gctaatcact ccgaacaggt cacaaaaaat taccttaaaa agtcataata ttaaattaga
    12601 ataaatatag ctgtgaggga aatatataca aatatattgg agcaaataaa ttgtacatac
    12661 aaatatttat tactaatttc tattgagacg aaatgaacca ctcggaacca tttgagcgaa
    12721 ccgaatcgcg cggaactaac gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt
    12781 tgcggagagc gggtgggaga cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg
    12841 aattatgaag agggcgcgcg atttgaaaag tatgtatata aaaaatatat cccggtgttt
    12901 tatgtagcga taaacgagtt tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag
    12961 aagacaaatc caaagtctac ttgtggggat gttcgaaggg gaaatacttg tattctatag
    13021 gtcatatctt gtttttattg gcacaaatat aattacatta gctttttgag ggggcaataa
    13081 acagtaaaca cgatggtaat aatggtaaaa aaaaaaaaca agcagttatt tcggatatat
    13141 gtcggctact ccttgcgtcg ggcccgaagt cttagagcca gatatgcgag cacccggaag
    13201 ctcacgatga gaatggccag acccacgtag tccagcggca gatcggcggc ggagaagtta
    13261 agcgtctcca ggatgacctt gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta
    13321 atttcgcccg gctccacgtc cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg
    13381 aaccatgaga ggtacgacaa ccatttgagg tatactggca ccgagcccga gttcaagaag
    13441 aagccgccaa agagcaggaa tggtatgata accggcggac ccacagacag cgccatcgag
    13501 gtcgaggagc tggcgcagga tattagatat ccgaaggacg ttgacacatt ggccaccaga
    13561 gtgaccagcg ccaggcagtt gaagaagtgc agcactccgg cccgcagtcc gatcatcgga
    13621 taggcaatcg ccgtgaagac cagtggcact gtgagaaaaa gcggcaattc ggcaatcgtt
    13681 ttgcccagaa agtatgtgtc acagcgataa agtcgacttc gggcctccct cataaaaact
    13741 ggcagctctg aggtgaacac ctaaatcgaa tcgattcatt agaaagttag taaattattg
    13801 aaatgcaaat gtattctaaa catgacttac atttatcgtg gcaaagacgt tttgaaaggt
    13861 catgttggtc aggaagagga agatggctcc gttgatattc atcacaccca cttgcgtgag
    13921 ttgttggccc aaaaagatga ggccaatcaa gatggcaacc atctgcaaat taaaatgtta
    13981 ctcgcatctc attaatattc gcgagttaaa tgaaatttat ttatcttctg caaaactata
    14041 aactatacat ctcattgaaa aaaactaaga agggtgtgga atcaggcaat tctatctaaa
    14101 atctagcgaa tttgtttcca agaattgtaa gcgttatatc atttgtttcc actggaacca
    14161 ctcaccgttg tctgaataag tcgcactttt acgaggagtg gttccttgag caccgacagc
    14221 caggatcgcc acaggaccgc ccggaactgc atgaaccagg tggccttgta ggtgtaccca
    14281 ttctccggct gctccagtgg cttctccaga tttttggtgg ccaacaactg ctccatatcc
    14341 cgggctactt tgctaatggc aaaattgtcg catatcttgg cgatccgatc acgggactcg
    14401 atctcccgtc cgggcacaac ggccaacacc tgtacgtaaa agtccgccgg attgtagttg
    14461 gtaggacact gggcacccac gctggatagg agttgagatg ttatgtaata ctagataccc
    14521 ttaataaaca catcgaactc actaggaaaa gaagtcgacg gcttcgctgg gagtgcccaa
    14581 gaaagctacc ctgccctcgg ccatcagaag gatcttgtca aagagctcaa acagctcgga
    14641 agacggctga tgaatggtca ggatgacggt cttgcccttc tgcgacagct tcttcagcac
    14701 ctggacgacg ctgtgggcgg taaaggagtc cagtccggag gtgggctcat cgcagatcag
    14761 aagcggcgga tcggttagag cctcggaggc gaatgccaga cgcttccttt ctccgccgga
    14821 cagacctttc accctgccgg gcacaccgat gatcgtgtgc tgacatttgc tgagcgaaag
    14881 ctcctggatc acctgatcca cgcgggccac tcgctgccga taggtcagat gtcgtggcat
    14941 ccgcaccatg gcttggaaaa tcaggtgttc cctggccgtt agggagccga taaagaggtc
    15001 atcctgctgg acataggcgc acctggcctg catctccttg gcgtccacag gttggccatt
    15061 gagcagtcgc atcccggatg gcgatacttg gatgccctgc ggcgatcgaa aggcaagggc
    15121 attcagcagg gtcgtctttc cggcaccgga actgcccatc acggccaaaa gttcgcccgg
    15181 ataggccacg ccgcaaactg agtttcaaat tggtaattgg accctttatt aagatttcac
    15241 acagatcagc cgactgcgaa tagaaactca ccgttcttga gcaaatgttt cctgggcgcc
    15301 ggtatgtgtc gctcgttgca gaatagtccg cgtgtccggt tgaccagctg ccgccatccg
    15361 gagcccggct gattgaccgc cccaaagatg tccatattgt gccaggcata ggtgaggttc
    15421 tcggctagtt ggccgctccc tgaaccggag tcctccggcg gactgggtgg ccggagcgtg
    15481 ccgtagtttt tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc cgctccgctg
    15541 tcaccctgca atgatagggg atctcaaata tcaactacaa gcgttatgct catctaaccc
    15601 cgaacaaaaa gtaccccgaa gtatcctacg aagtaggttt atacttttat ttattttttg
    15661 tgcatctagg atcagcttaa aatatctggt tgttatattt tttgtaaaaa agaatatagt
    15721 cgaaaatgaa tgcctttaga tgtcttgatc atgatatgat ctcaaaaatt gtcttatata
    15781 gcgagaacag ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc aattgcggtt
    15841 tgggattttt gtgggtcgca gttctcacgc cgcagacaat ttgatgttgc aatcgcagtt
    15901 cctatagatc aagtgaactt aagatgtatg cacatgtact actcacattg ttcagatgct
    15961 cggcagatgg gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct tggcccattg
    16021 ccgggatttt tcacactttc ccctgcttac ccacccaaaa ccaatcacca ccccaatcac
    16081 tcaaaaaaca aacaaaaata agaagcgaga ggagttttgg cacagcactt tgtgtttaat
    16141 tgatggcgta aaccgcttgg agcttcgtca cgaaaccgct gacaaaatgc aactgaaggc
    16201 ggacattgac gctacgtaac gctacaaacg gtggcgaaag agatagcgga cgcagcggcg
    16261 aaagagacgg cgatatttct gtggacagag aaggaggcaa acagcgctga ctttgagtgg
    16321 aatgtcattt tgagtgagag gtaatcgaaa gaacctggta catcaaatac ccttggatcg
    16381 aagtaaattt aaaactgatc agataagttc aatgatatcc agtgcagtaa aaaaaaaaaa
    16441 tgtttttttt atctactttc cgcaaaaatg ggttttatta acttacatac atactag
//
