LOCUS       sequence-361790-       17141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion 
            protein under the maternal tubulin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17141)
  AUTHORS   Singh AP, Wu P, Ryabichko S, Raimundo J, Swan M, Wieschaus E, Gregor
            T, Toettcher JE
  TITLE     Optogenetic control of the Bicoid morphogen reveals fast and slow 
            modes of gap gene regulation.
  JOURNAL   Cell Rep. 2022 Mar 22;38(12):110543. doi: 
            10.1016/j.celrep.2022.110543.
  PUBMED    35320726
REFERENCE   2  (bases 1 to 17141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-03-22"; volume: "38(12):110543. doi"; pages: " 
            10.1016/j.celrep.2022.110543"
COMMENT     Sequence Label: pBABR mCherry-Bcd-LEXY
FEATURES             Location/Qualifiers
     source          1..17141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2443..3150
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2588..2606)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2856..2875
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    5140..5187
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    7922..7969
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8813..8829
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8813..8829
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    9199..9268
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(9432..9633,9634..9664)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10202..10221)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10321..10343
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10381..10399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10467..10571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10572..10640,10641..11432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10790..10809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11603..12191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12092..12111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(12426..12456,12457..13011)
                     /label=P element 5' end
     CDS             complement(join(13790..14404,14475..14606,14810..15125,
                     15187..15841,15916..16189,16591..16662))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     17114..17132
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 aattggccgc tctagccccc cctcgaatgt tctctctctt ctcttctctc tctctttctc
       61 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctaag
      121 ggtcgagtcg atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt
      181 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaaccggtcc acggccgctt
      241 ggagggagat tagcaaaacg cagcgcgtga tcaaggtgac aatgaagcca ccgaccacca
      301 cggtggcgcc caacaaggca cgagtggttt gcatgtccga cacgcactcc ctgacgccct
      361 acatcaagtt cgatatacca gatggcgata tattcataca tgccggcgat tttaccaagt
      421 gcggccaact ggaggaggtg gaggagttta acacctggat tggagcactg ccccatcgcc
      481 acaagatcgt gattgctggc aatcatgagc tcagcttcga tagaaccttc acccatcctt
      541 tccagaagag caaaagtcat gcttcgagca gcaaacacac cggcatgtcc atcctcgatg
      601 atctgcccac tttgggcaac gccaaggaga acttggagag cgctgtgcaa acgcagaatg
      661 tccgcgatgt tctgaccaac tgtcggtatt tggaggatga actacttgag atttggggta
      721 ttcaaatata tggctcgcca tggcaaccgg aattctgtcg ctgggccttt aatgttccac
      781 gtggaactgc ctgtttggat aagtggaatc aaatcccgga aggcatcgat atcctggtca
      841 cccacacgcc gcccgtgggt cacggcgatc tctgctgttc cggcgtacga gccggttgtg
      901 tggagctact cagcacagtg cagcaacgag tgcgcccgaa ataccatgtc ttcggacatg
      961 tacacgaagg ctacggcatc accagcgatg gcaggatcat ctttgtgaat gcctcaacgt
     1021 gtgacatcaa ctacctaccc aacaatgccc ccatcgtttt cgatgtgacg ctgccccccg
     1081 gatttcgcaa ggattagcat gcctagatga ttatttattg tgtcacaggt tcaatgaatt
     1141 tattttcgtc ccactttatg tttatgttaa ggtatttcgt ctgtaaagat tttgaagcac
     1201 aattgtaggt gtctagagag atagaattca attcgcacga aattaaaaaa agaaattcac
     1261 acttaagatg ccataaaata tttattcaag tagagtgcat ttgtcaatat atgaatttgt
     1321 aaaaataaac aagttttgaa gacacctaaa cttttgaaga ttttcaatat gatattatat
     1381 agtttttaat aataatttaa gtaaaggtaa agcaaatcct gagatacata catactattt
     1441 caaattagta tcgataagcc gccagaaatg ggttggaaaa accttggaat gattattggt
     1501 tagcagccct gtctcccaat ttgacattcc tctttggcgc tctgtaacgg cgcttttcca
     1561 gtgactcccc aatcagcgaa ccaactaacc tgaggctgca cttttcgcgt ggcgaccaaa
     1621 aaaattataa aaaaaaggtc ccgcattaga ttttaatttg tatacaccca attttagtcc
     1681 agcaaaaaaa aaaactaatc aattggtgag aagaagaaaa actaattagt ttaagaattg
     1741 ctgaaagcgg gaagtgaaat ttaaaatcag aaaagatgtg taagaagatg tgaaatgaaa
     1801 aggtgtggga agtgaacatg aataaatgtt cattgaaaat gaacagctaa ttgcgaattt
     1861 tttgaaactt aactgttaac tgttactgct cacaaatcct tgaaaaatat taagatcgat
     1921 cttagaattg tttatttaaa atgaattcct cgtatgtatt ataacagaca atcaagttta
     1981 ttaaagaata tttaaacttg tatttttttt tgagtttgat aaaatgtccc aaatataaaa
     2041 gagtattacc ttttcgtcat ataatactgt tcttaatatt agttttagtt agtaatgtgt
     2101 ttataaagta aattacaaag ggaggaccta acctaacctt aaacaatggc gccttctctt
     2161 gcccagctca tcacttttat tgtttcaccc gccagcgcga agtagtgtcc atccagattg
     2221 ggacggtacc atctcttcgc tcatccctaa ataacggcac tctgcagatg cgaagcagtg
     2281 gatcgcaaaa acgcaaaatg tgggcgaaat aagttcgcga gcgtctcgaa agtatccggt
     2341 tactgaaaat acaagaaagt ttccacactc ctttgccatt tttccgcgcg gcgcttggaa
     2401 attcgtaaag ataacgcggc ggagtgtttg gggctagcaa agatggtgag caagggcgag
     2461 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2521 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2581 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2641 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     2701 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2761 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     2821 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     2881 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2941 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     3001 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     3061 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     3121 tccaccggcg gcatggacga gctgtacaag gagttcgact ccgcaggctc cgccggaagt
     3181 gcgggaggta gtagtgcgca accgccgcca gatcaaaact tttaccatca tccgctgccc
     3241 cacacgcaca cacatccgca tccgcactcc catccgcatc cgcactcgca tccgcaccca
     3301 catcaccaac atccgcagct tcagttgccg ccacaattcc gaaatccctt cgatttgctt
     3361 ttcgatgagc gaacgggagc gataaactac aactacatac gtccgtatct gcccaaccag
     3421 atgcccaagc cagaggagct gcccgactct ctggtgatgc ggcgaccacg tcgcacccgc
     3481 accactttta ccagctctca aatagcagag ctggagcagc actttctgca gggacgatac
     3541 ctcacagccc cccgacttgc ggatctgtca gcgaaactag ccctgggcac agcccaggtg
     3601 aagatatggt ttaagaaccg tcggcgtcgt cacaagatcc aatcggatca gcacaaggac
     3661 cagtcctacg aggggatgcc tctctcgccg ggtatgaaac agagcgatgg cgatcccccc
     3721 agcttgcaga ctcttagctt gggtggaggg gccacgccca acgctttgac tccgtcaccc
     3781 acgccctcaa cgcccactgc acacatgacg gagcactaca gcgagtcatt caacgcctac
     3841 tacaactaca atggaggcca caatcacgcc caggccaatc gtcacatgca catgcagtat
     3901 ccttccggag ggggggcagg acctgggtcg accaatgtca atggcggcca gttcttccag
     3961 cagcagcagg tccataatca ccagcagcaa ctgcaccacc agggcaacca cgtgccgcac
     4021 cagatgcagc agcagcaaca gcaggctcag cagcagcaat accatcactt tgacttccag
     4081 caaaagcaag ccagcgcctg tcgcgtcctg gtcaaggacg aaccggaggc cgactacaac
     4141 ttcaacagct cgtactacat gcgatcggga atgtctggcg ccactgcatc ggcatccgct
     4201 gtggcccgag gcgctgcctc gccgggctcc gaggtctacg agccattaac acccaagaat
     4261 gacgaaagtc cgagtctgtg tggcatcggc atcggcggac cttgcgccat cgccgttggc
     4321 gagacggagg cggccgacga catggacgac ggaacgagca agaagacgac gctacagatc
     4381 ttggagcctt tgaagggtct ggacaagagc tgcgacgatg gcagtagcga cgacatgagc
     4441 accggaataa gagccttagc aggaaccgga aatcgtggag cggcatttgc caaatttggc
     4501 aagccttcgc ccccacaagg ccctcagccg cccctcggga tggggggcgt ggccatgggc
     4561 gaatcgaacc aatatcaatg cacgatggat acgataatgc aagcgtataa tccccatcgg
     4621 aacgccgcgg gcaactcgca gtttgcctac tgcttcaata agggtggatc tggaggttca
     4681 ggtggaagtt tggctactac acttgaacgt attgagaaga actttgtcat tactgaccca
     4741 agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt gacagaatat
     4801 agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga aactgatcgc
     4861 gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac tgttcagctg
     4921 attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca gcctatgcga
     4981 gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac tgagcatgtc
     5041 cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga aaatattgac
     5101 gagctgctga aggagctggc cgacctgaac ctggactagg aagtacctat tccgaagttc
     5161 ctattctcta gaaagtatag gaacttccct ggatgagagg cgtgttagag agtttcatta
     5221 gctttaggtt aaccactgtt gttcctgatt gtacaaatac caagtgattg tagatatcta
     5281 cgcgtagaaa gttaggtcta gtcctaagat ccgtgtaaat ggttcccagg gaagttttat
     5341 gtactagcct agtcagcagg ccgcacggat tccagtgcat atcttagtga tactccagtt
     5401 aactctatac tttccctgca atacgctatt cgccttagat gtatctgggt ggctgctcca
     5461 ctgaagcccg ggaatatgca accagttaca tttgaggcca tttgggctta agcgtattcc
     5521 atggaaagtt atcgtcccac atttcggaaa ttatattccg agccagcaag aaaatcttct
     5581 ctgttacaat ttgacatagc taaaaactgt actaatcaaa atgaaaaatg tttctcttgg
     5641 gcgtaatctc atacaatgat tacccttaaa gatcgaacat ttaaacaata atatttgata
     5701 tgatattttc aatttctatg ctatgccaaa gtgtctgaca taatcaaaca tttgcgcatt
     5761 ctttgaccaa gaatagtcag caaattgtat tttcaatcaa tgcagaccat ttgtttcaga
     5821 ttcggagatt ttttgctgcc aaacggaata actatcatag ctcacattct aattacatca
     5881 ctaagaagag cattgcaatc tgttaggcct caagtttaat tttaaaatgc tgcacctttg
     5941 atgttgtctc tttaagcttt gtatttttaa ttacgaaaat atataagaac tactctactc
     6001 gggtaaattg tgactaacta cacataacta catacttagc ccatatttcc gtccctttct
     6061 agaatgaacg aaaacagtat ctggttttcc cgaaaatctt atgaatttaa aaatgcactt
     6121 tattgcacat actcacacat gcctgccata aaatatgatt cgcgagcggc cgcggatcta
     6181 attcaattag agactaattc aattagagct aattcaatta ggatccaagc ttatcgattt
     6241 cgaaccctcg accgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     6301 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     6361 gaacaagcta aacaatcggg cggccgcact agagccggtc gccaccatga ggtcttccaa
     6421 gaatgttatc aaggagttca tgaggtttaa ggttcgcatg gaaggaacgg tcaatgggca
     6481 cgagtttgaa atagaaggcg aaggagaggg gaggccatac gaaggccaca ataccgtaaa
     6541 gcttaaggta accaaggggg gacctttgcc atttgcttgg gatattttgt caccacaatt
     6601 tcagtatgga agcaaggtat atgtcaagca ccctgccgac ataccagact ataaaaagct
     6661 gtcatttcct gaaggattta aatgggaaag ggtcatgaac tttgaagacg gtggcgtcgt
     6721 tactgtaacc caggattcca gtttgcagga tggctgtttc atctacaagg tcaagttcat
     6781 tggcgtgaac tttccttccg atggacctgt tatgcaaaag aagacaatgg gctgggaagc
     6841 cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa ggagagattc ataaggctct
     6901 gaagctgaaa gacggtggtc attacctagt tgaattcaaa agtatttaca tggcaaagaa
     6961 gcctgtgcag ctaccagggt actactatgt tgactccaaa ctggatataa caagccacaa
     7021 cgaagactat acaatcgttg agcagtatga aagaaccgag ggacgccacc atctgttcct
     7081 ttagcggcca tcgaattcga gctcgcccac taagcgtcgc gccacttcaa cgctcgatgg
     7141 gagcgtcatt ggtgggcggg gtaaccgtcg aaatcagtgt ttacgcttcc aatcgcaaca
     7201 aaaaattcac tgcaacactg aaaagcatac gaaaacgatg aagattgtac gagaaaccat
     7261 aaagtatttt atccacaaag acacgtatag cagaaaagcc aagttaactc ggcgataagt
     7321 tgtgtacaca agaataaaat cggccagatt cagtgttgtc agaaataaga aaaccccact
     7381 atgtttttct ttgccttttc tttctcccag cgatcattca tttcgtggtg aaagaacggg
     7441 gtcattgcac ggagtttcga ctgcgggaaa gcagagctgc cgttcacttc gtctataatt
     7501 agcgctttct attttccccg attcgggccg ctgctgcgct tttccgcctg ctgtttgtgg
     7561 caagtgtagc agcaggctgt gcacgcagtg tggcatgcac ttggctttcc accgttggta
     7621 tcgattctct gggacgatga gtcattcctt tcggggccac agcataatcg ttgccagctc
     7681 accgaaatgg tgacttcatt tcttaactgc cgtcaagcat gcgattgtac atacatacat
     7741 atttatatat gtacatattt atgtgactat ggtaggtcga tataatagca atcaacgcaa
     7801 gcaaatgtgt cagtcctgct tacaggaacg attctattta gtaattttcg ttgtataaag
     7861 taattatgta tgtatgtaag ccccataaat ctgaaacaat taggcaaaac catgcgaagc
     7921 tgaagtacct attccgaagt tcctattctc tagaaagtat aggaacttcc atatggcggc
     7981 cgccattcgt agaataggga tccccagcag tcgattcact agccagctgc ctctccaatc
     8041 agcctgtcca aactatccat catgagtttc gttttgataa tttaatatat acaagtataa
     8101 attttttttt tttggtttcg cgccttctgt tctttgattc ctgataatcc tgtacttatc
     8161 cgtgcatttg aattaaacaa aaagtagcca acaccagcga ttgttatgta cgacagtgta
     8221 gtgtgtgaga tgttggtgcc agataacacg atttcccgaa aaactccttt cataagtatt
     8281 aactcattga atatacatat atattaccta tatacaaata catacttata caagaatgtg
     8341 gcaataaacc tttgtttttc ttacactcga gaacgtgcgc acgtccaacc aaacagatgt
     8401 ctgcagaaat ccttcagcca aaagcaactg atcaaacaat agaaactttg gaatatgtac
     8461 cccgcatggc taactaagca tgatcactag catgatgtga taaaagtatg tagcttcaaa
     8521 tgtggaggga aattgaaaga gatttcggaa attagtcaaa attttcttgg aaccttcgac
     8581 aagctgacta atgagttcat gtccaaaaga ctcgaacttg taaactcata tcttatctca
     8641 cattattttt gattcgaaga tatttcagct tctgcaaata tatgtttttc aagaatttat
     8701 ttaaaaaaaa aatcaattaa atagatggct taatgttcga aacactcaac gttgtatatt
     8761 acacactttt tggacaacta ttaatttaaa agaaaacacg aacatttgaa aatctagaac
     8821 tagtggatct ggatccagac tgcagagaag atctatagaa ttcagagagg ccggcagaga
     8881 ggctagcagg catgcatcta gagtcgacct cgaacgttaa cgttaacgta acgttaactc
     8941 gaggagcttg ataacattat acctaaaccc atggtcaaga gtaaacattt ctgcctttga
     9001 agttgagaac acaattaagc atcccctggt taaacctgct tgatagtgca ggctatacag
     9061 aactgcgcta gtgactccta tccgcctgcg tttctctgcc tgctcacggg tcatatgcgt
     9121 gctttggagc tgagggtgcg gcggattggc gtcgacgatg taggtcacgg cgggcatgtc
     9181 gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
     9241 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
     9301 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
     9361 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg acgatgtagg tcacggcggg
     9421 cagccaagct ttgcgtactc gcaaattatt aaaaataaaa ctttaaaaat aatttcgtct
     9481 aattaatatt atgagttaat tcaaacccca cggacatgct aagggttaat caacaatcat
     9541 atcgctgtct cactcagact caatacgaca ctcagaatac tattcctttc actcgcactt
     9601 attgcaagca tacgttaagt ggatgtctct tgccgacggg accaccttat gttatttcat
     9661 catggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt
     9721 cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt
     9781 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
     9841 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
     9901 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
     9961 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
    10021 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt
    10081 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg
    10141 actgtcgtta gttccgcgcg attcggtgcg gtatttcaca ccgcatatgg tgcactctca
    10201 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    10261 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    10321 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
    10381 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    10441 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10501 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10561 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10621 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10681 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10741 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10801 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10861 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10921 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10981 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11041 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11101 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11161 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11221 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11281 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11341 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11401 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11461 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11521 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11581 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    11761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12181 gcctatggaa aaacgccttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
    12241 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
    12301 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
    12361 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
    12421 tggcccatga tgaaataaca taaggtggtc ccgtcgatag ccgaagctta ccgaagtata
    12481 cacttaaatt cagtgcacgt ttgcttgttg agaggaaagg ttgtgtgcgg acgaattttt
    12541 ttttgaaaac attaaccctt acgtggaata aaaaaaaatg aaatattgca aattttgctg
    12601 caaagctgtg actggagtaa aattaattca cgtgccgaag tgtgctatta agagaaaatt
    12661 gtgggagcag agccttgggt gcagccttgg tgaaaactcc caaatttgtg atacccactt
    12721 taatgattcg cagtggaagg ctgcacctgc aaaaggtcag acatttaaaa ggaggcgact
    12781 caacgcagat gccgtaccta gtaaagtgat agagcctgaa ccagaaaaga taaaagaagg
    12841 ctataccagt gggagtacac aaacagagta agtttgaata gtaaaaaaaa tcatttatgt
    12901 aaacaataac gtgactgtgc gttaggtcct gttcattgtt taatgaaaat aagagcttga
    12961 gggaaaaaat tcgtactttg gagtacgaaa tgcgtcgttt agagcagcag ccgaattaat
    13021 tctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg
    13081 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac
    13141 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac
    13201 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga
    13261 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa
    13321 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac
    13381 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
    13441 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
    13501 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
    13561 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
    13621 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
    13681 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
    13741 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
    13801 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
    13861 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
    13921 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
    13981 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
    14041 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca
    14101 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc
    14161 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc
    14221 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga
    14281 agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg
    14341 tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga
    14401 acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc
    14461 taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag
    14521 aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag
    14581 atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat
    14641 attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt
    14701 gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt
    14761 tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa
    14821 taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga
    14881 ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca
    14941 gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa
    15001 tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca
    15061 caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac
    15121 ccacgctgga taggagttga gatgttatgt aatactagat acccttaata aacacatcga
    15181 actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc
    15241 tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg
    15301 gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg
    15361 gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt
    15421 agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg
    15481 ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga
    15541 tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg
    15601 aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag
    15661 gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg
    15721 gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc
    15781 tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa
    15841 actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg
    15901 cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt
    15961 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
    16021 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
    16081 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
    16141 gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata
    16201 ggggatctca aatatcaact acaagcgtta tgctcatcta accccgaaca aaaagtaccc
    16261 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
    16321 ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt
    16381 tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca
    16441 gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt
    16501 cgcagttctc acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga
    16561 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
    16621 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
    16681 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
    16741 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
    16801 ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg
    16861 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
    16921 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
    16981 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
    17041 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
    17101 tttccgcaaa aatgggtttt attaacttac atacatacta g
//
