LOCUS       sequence-361805-       11261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hNav1.8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   McCollum MM, Larmore M, Ishihara S, Ng LCT, Kimura LF, Guadarrama E,
            Ta MC, Vien TN, Frost GB, Scheidt KA, Miller RE, DeCaen PG
  TITLE     Targeting the tamoxifen receptor within sodium channels to block 
            osteoarthritic pain.
  JOURNAL   Cell Rep. 2022 Aug 23;40(8):111248. doi: 
            10.1016/j.celrep.2022.111248.
  PUBMED    36001977
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-08-23"; volume: "40(8):111248. doi"; pages: " 
            10.1016/j.celrep.2022.111248"
COMMENT     Sequence Label: hNav1.8_pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..11261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        491..694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     645..665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     739..758
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        739..757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      811..820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6685..6708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6728..6745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6734..6958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7004..7432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7091..7110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7301..7322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7441..7461)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7446..7775
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7626..7761
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7688..7707
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7842..8636
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7896..7915)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8506..8525
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8810..8931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8847..8866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8901..8920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8980..8996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8980..8996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8993..9015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9004..9020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9028..9034,9035..9052,9053..9058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9073..9094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9211..9228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9382..9970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9462..9481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10141..10932,10933..11001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10764..10783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11002..11106)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11181..11200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
       61 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      601 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      661 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      721 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt
      781 aaacttaagc ttggtaccga gctcggatcc gccaccatgg aattccccat tggatccctc
      841 gaaactaaca acttccgtcg ctttactccg gagtcactgg tggagataga gaagcaaatt
      901 gctgccaagc agggaacaaa gaaagccaga gagaagcata gggagcagaa ggaccaagaa
      961 gagaagcctc ggccccagct ggacttgaaa gcctgcaacc agctgcccaa gttctatggt
     1021 gagctcccag cagaactgat cggggagccc ctggaggatc tagatccgtt ctacagcaca
     1081 caccggacat ttatggtgct gaacaaaggg aggaccattt cccggtttag tgccactcgg
     1141 gccctgtggc tattcagtcc tttcaacctg atcagaagaa cggccatcaa agtgtctgtc
     1201 cactcgtggt tcagtttatt tattacggtc actattttgg ttaattgtgt gtgcatgacc
     1261 cgaactgacc ttccagagaa aattgaatat gtcttcactg tcatttacac ctttgaagcc
     1321 ttgataaaga tactggcaag aggattttgt ctaaatgagt tcacgtacct gagagatcct
     1381 tggaactggc tggattttag cgtcattacc ctggcatatg ttggcacagc aatagatctc
     1441 cgtgggatct caggcctgcg gacattcaga gttcttagag cattaaaaac agtttctgtg
     1501 atcccaggcc tgaaggtcat tgtgggggcc ctgattcact cagtgaagaa actggctgat
     1561 gtgaccatcc tcaccatctt ctgcctaagt gtttttgcct tggtggggct gcaactcttc
     1621 aagggcaacc tcaaaaataa atgtgtcaag aatgacatgg ctgtcaatga gacaaccaac
     1681 tactcatctc acagaaaacc agatatctac ataaataagc gaggcacttc tgacccctta
     1741 ctgtgtggca atggatctga ctcaggccac tgccctgatg gttatatctg ccttaaaact
     1801 tctgacaacc cggattttaa ctacaccagc tttgattcct ttgcttgggc tttcctctca
     1861 ctgttccgcc tcatgacaca ggattcctgg gaacgcctct accagcagac cctgaggact
     1921 tctgggaaaa tctatatgat cttttttgtg ctcgtaatct tcctgggatc tttctacctg
     1981 gtcaacttga tcttggctgt agtcaccatg gcgtatgagg agcagaacca ggcaaccact
     2041 gatgaaattg aagcaaagga gaagaagttc caggaggccc tcgagatgct ccggaaggag
     2101 caggaggtgc tagcagcact agggattgac acaacctctc tccactccca caatggatca
     2161 cctttaacct ccaaaaatgc cagtgagaga aggcatagaa taaagccaag agtgtcagag
     2221 ggctccacag aagacaacaa atcaccccgc tctgatcctt acaaccagcg caggatgtct
     2281 tttctaggcc tcgcctctgg aaaacgccgg gctagtcatg gcagtgtgtt ccatttccgg
     2341 tcccctggcc gagatatctc actccctgag ggagtcacag atgatggagt ctttcctgga
     2401 gaccacgaaa gccatcgggg ctctctgctg ctgggtgggg gtgctggcca gcaaggcccc
     2461 ctccctagaa gccctcttcc tcaacccagc aaccctgact ccaggcatgg agaagatgaa
     2521 caccaaccgc cgcccactag tgagcttgcc cctggagctg tcgatgtctc ggcattcgat
     2581 gcaggacaaa agaagacttt cttgtcagca gaatacttag atgaaccttt ccgggcccaa
     2641 agggcaatga gtgttgtcag tatcataacc tccgtccttg aggaactcga ggagtctgaa
     2701 cagaagtgcc caccctgctt gaccagcttg tctcagaagt atctgatctg ggattgctgc
     2761 cccatgtggg tgaagctcaa gacaattctc tttgggcttg tgacggatcc ctttgcagag
     2821 ctcaccatca ccttgtgcat cgtggtgaac accatcttca tggccatgga gcaccatggc
     2881 atgagcccta ccttcgaagc catgctccag ataggcaaca tcgtctttac catatttttt
     2941 actgctgaaa tggtcttcaa aatcattgcc ttcgacccat actattattt ccagaagaag
     3001 tggaatatct ttgactgcat catcgtcact gtgagtctgc tagagctggg cgtggccaag
     3061 aagggaagcc tgtctgtgct gcggagcttc cgcttgctgc gcgtattcaa gctggccaaa
     3121 tcctggccca ccttaaacac actcatcaag atcatcggaa actcagtggg ggcactgggg
     3181 aacctcacca tcatcctggc catcattgtc tttgtctttg ctctggttgg caagcagctc
     3241 ctaggggaaa actaccgtaa caaccgaaaa aatatctccg cgccccatga agactggccc
     3301 cgctggcaca tgcacgactt cttccactct ttcctcattg tcttccgtat cctctgtgga
     3361 gagtggattg agaacatgtg ggcctgcatg gaagttggcc aaaaatccat atgcctcatc
     3421 cttttcttga cggtgatggt gctagggaac ctggtggtgc ttaacctgtt catcgccctg
     3481 ctattgaact ctttcagtgc tgacaacctc acagccccgg aggacgatgg ggaggtgaac
     3541 aacctgcagg tggccctggc acggatccag gtctttggcc atcgtaccaa acaggctctt
     3601 tgcagcttct tcagcaggtc ctgcccattc ccccagccca aggcagagcc tgagctggtg
     3661 gtgaaactcc cactctccag ctccaaggct gagaaccaca ttgctgccaa cactgccagg
     3721 gggagctctg gagggctcca agctcccaga ggccccaggg atgagcacag tgacttcatc
     3781 gctaatccga ctgtgtgggt ctctgtgccc attgctgagg gtgaatctga tcttgatgac
     3841 ttggaggatg atggtgggga agatgctcag agcttccagc aggaagtgat ccccaaagga
     3901 cagcaggagc agctgcagca agtcgagagg tgtggggacc acctgacacc caggagccca
     3961 ggcactggaa catcttctga ggacctggct ccatccctgg gtgagacgtg gaaagatgag
     4021 tctgttcctc aggtccctgc tgagggagtg gacgacacaa gctcctctga gggcagcacg
     4081 gtggactgcc tagatcctga ggaaatcctg aggaagatcc ctgagctggc agatgacctg
     4141 gaagaaccag atgactgctt cacagaagga tgcattcgcc actgtccctg ctgcaaactg
     4201 gataccacca agagtccatg ggatgtgggc tggcaggtgc gcaagacttg ctaccgtatc
     4261 gtggagcaca gctggtttga gagcttcatc atcttcatga tcctgctcag cagtggatct
     4321 ctggcctttg aagactatta cctggaccag aagcccacgg tgaaagcttt gctggagtac
     4381 actgacaggg tcttcacctt tatctttgtg ttcgagatgc tgcttaagtg ggtggcctat
     4441 ggcttcaaaa agtacttcac caatgcctgg tgctggctgg acttcctcat tgtgaatatc
     4501 tcactgataa gtctcacagc gaagattctg gaatattctg aagtggctcc catcaaagcc
     4561 cttcgaaccc ttcgcgctct gcggccactg cgggctcttt ctcgatttga aggcatgcgg
     4621 gtggtggtgg atgccctggt gggcgccatc ccatccatca tgaatgtcct cctcgtctgc
     4681 ctcatcttct ggctcatctt cagcatcatg ggtgtgaacc tcttcgcagg gaagttttgg
     4741 aggtgcatca actataccga tggagagttt tcccttgtac ctttgtcgat tgtgaataac
     4801 aagtctgact gcaagattca aaactccact ggcagcttct tctgggtcaa tgtgaaagtc
     4861 aactttgata atgttgcaat gggttacctt gcacttctgc aggtggcaac ctttaaaggc
     4921 tggatggaca ttatgtatgc agctgttgat tcccgggagg tcaacatgca acccaagtgg
     4981 gaggacaacg tgtacatgta tttgtacttt gtcatcttca tcatttttgg aggcttcttc
     5041 acactgaatc tctttgttgg ggtcataatt gacaacttca atcaacagaa aaaaaagtta
     5101 gggggccagg acatcttcat gacagaggag cagaagaaat actacaatgc catgaagaag
     5161 ttgggctcca agaagcccca gaagcccatc ccacggcccc tgaacaagtt ccagggtttt
     5221 gtctttgaca tcgtgaccag acaagctttt gacatcacca tcatggtcct catctgcctc
     5281 aacatgatca ccatgatggt ggagactgat gaccaaagtg aagaaaagac gaaaattctg
     5341 ggcaaaatca accagttctt tgtggccgtc ttcacaggcg aatgtgtcat gaagatgttc
     5401 gctttgaggc agtactactt cacaaatggc tggaatgtgt ttgacttcat tgtggtggtt
     5461 ctctccattg cgagcctgat tttttctgca attcttaagt cacttcaaag ttacttctcc
     5521 ccaacgctct tcagagtcat ccgcctggcc cgaattggcc gcatcctcag actgatccga
     5581 gcggccaagg ggatccgcac actgctcttt gccctcatga tgtccctgcc tgccctcttc
     5641 aacatcgggc tgttgctatt ccttgtcatg ttcatctact ctatcttcgg tatgtccagc
     5701 tttccccatg tgaggtggga ggctggcatc gacgacatgt tcaacttcca gaccttcgcc
     5761 aacagcatgc tgtgcctctt ccagattacc acgtcggccg gctgggatgg cctcctcagc
     5821 cccatcctca acacagggcc cccctactgt gaccccaatc tgcccaacag caatggcacc
     5881 agaggggact gtgggagccc agccgtaggc atcatcttct tcaccaccta catcatcatc
     5941 tccttcctca tcgtggtcaa catgtacatt gcagtgattc tggagaactt caatgtggcc
     6001 acggaggaga gcactgagcc cctgagtgag gacgactttg acatgttcta tgagacctgg
     6061 gagaagtttg acccagaggc cactcagttt attacctttt ctgctctctc ggactttgca
     6121 gacactctct ctggtcccct gagaatccca aaacccaatc gaaatatact gatccagatg
     6181 gacctgcctt tggtccctgg agataagatc cactgcttgg acatcctttt tgctttcacc
     6241 aagaatgtcc taggagaatc cggggagttg gattctctga aggcaaatat ggaggagaag
     6301 tttatggcaa ctaatctttc aaaatcatcc tatgaaccaa tagcaaccac tctccgatgg
     6361 aagcaagaag acatttcagc cactgtcatt caaaaggcct atcggagcta tgtgctgcac
     6421 cgctccatgg cactctctaa caccccatgt gtgcccagag ctgaggagga ggctgcatca
     6481 ctcccagatg aaggttttgt tgcattcaca gcaaatgaaa attgtgtact cccagacaaa
     6541 tctgaaactg cttctgccac atcattccca ccgtcctatg agagtgtcac tagaggcctt
     6601 agtgatagag tcaacatgag gacatctagc tcaatacaaa atgaagatga agccaccagt
     6661 atggagctga ttgcccctgg gcccgattac aaggatgacg acgataagtg ataaacccgc
     6721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6901 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6961 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     7021 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7081 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7141 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7201 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7261 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7321 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7381 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     7441 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7501 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     7561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     7621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7681 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     7741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     7801 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     7861 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7921 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7981 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     8041 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8101 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     8161 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     8221 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8281 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     8341 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     8401 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8461 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     8521 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8581 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     8641 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     8701 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8761 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8941 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9001 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9061 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9181 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11101 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    11161 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    11221 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga g
//
