LOCUS       sequence-36188-e       12760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Qi Y, Katagiri F
  TITLE     Purification of low-abundance Arabidopsis plasma-membrane protein 
            complexes and identification of candidate components.
  JOURNAL   Plant J. 2009 Mar;57(5):932-44. Epub 2008 Dec 4.
  PUBMED    19000159
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2009-03"; volume: "57(5)"; pages: "932-44. Epub 2008 Dec 4"
COMMENT     Sequence Label: pMDC-pRPS2-HPB
FEATURES             Location/Qualifiers
     source          1..12760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..27)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11..27)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(24..46)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    35..51
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(59..65,66..83,84..89))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    104..125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        316..993
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1061..2086
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2126..2300
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2378..2402
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2827..3621
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3708..4296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4197..4216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4450..4467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4482..4622
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4548..4567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4708..4730
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4966..5160
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5226..6293)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6727..7356)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8868..8890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8882..8899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10401..10525
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10550..10555,10556..10573,10574..10580)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10634..11314
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(10700..10719)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             11634..11939
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11818..11837
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11980..12104)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             12133..12159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12169..12192
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
       61 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      121 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      181 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc
      241 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      301 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      361 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      421 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      481 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      541 gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacactc tcgtctactc
      601 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
      661 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
      721 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
      781 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
      841 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
      901 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccttc ctctatataa
      961 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
     1021 tctctctcga gctttcgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac
     1081 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     1141 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     1201 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     1261 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta gcgagagcct
     1321 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     1381 actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg cggccgatct
     1441 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     1501 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     1561 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     1621 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     1681 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     1741 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     1801 ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg cgtatatgct
     1861 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     1921 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     1981 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     2041 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta gatgccgacc
     2101 ggatctgtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     2161 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     2221 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     2281 ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg
     2341 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     2401 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     2461 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     2521 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     2581 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     2641 ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     2701 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     2761 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     2821 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     2881 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     2941 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     3001 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     3061 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     3121 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     3181 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     3241 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     3301 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     3361 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     3421 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     3481 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     3541 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     3601 ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt
     3661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4321 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4381 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4441 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     4501 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     4561 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     4621 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4681 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4741 catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac
     4801 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
     4861 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
     4921 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
     4981 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
     5041 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
     5101 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
     5161 ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
     5221 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
     5281 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
     5341 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
     5401 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
     5461 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
     5521 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
     5581 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
     5641 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
     5701 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
     5761 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
     5821 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
     5881 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
     5941 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
     6001 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
     6061 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc
     6121 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
     6181 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
     6241 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc
     6301 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
     6361 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
     6421 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
     6481 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
     6541 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
     6601 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
     6661 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
     6721 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
     6781 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
     6841 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
     6901 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
     6961 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
     7021 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
     7081 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
     7141 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
     7201 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
     7261 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
     7321 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
     7381 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
     7441 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
     7501 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
     7561 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
     7621 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
     7681 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
     7741 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
     7801 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
     7861 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
     7921 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
     7981 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
     8041 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
     8101 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
     8161 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
     8221 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
     8281 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
     8341 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
     8401 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
     8461 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
     8521 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
     8581 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagctttt tcaaattcca gtatataatt ttcttctcaa
     8941 tttatcctta agggaaatga agagttggtc atggaagtcc attaaattcg accaaatttt
     9001 tagttttttt tattgaccag aaaagacagt acaaaacata aaattattgt tgtcgcagta
     9061 tatatataaa ataaaaagat gattccgaag gtattttgat ggatagactc atggacataa
     9121 ttatgcaacg acccaaagaa catgaaagaa tccgtccaat tttttaaaat aaaataaaaa
     9181 tgtgaatagg tcccacgttt agcagaaaac aaaaaccggt ggatggtcga cacgtagacg
     9241 aagaaaatgg gaaggagaga gggtgacgtg tacggtttaa gatgtgttag ttgggtggcg
     9301 ggagagattt gatcgaaaga aaaaaaggtt cattgcactg acaaagacag gtcccccttt
     9361 tatattattt ttctttcgtc ccattaaatt ccgtaaatat cttgtcttta aactctcttt
     9421 tttcctctcc tcattattat tctattttct ctaatatctc aataaatatt tattcgtcaa
     9481 cagatagata aaaaagaagt atttattcat gttccatatg tttgatgcca cttgtgcggt
     9541 tttctttgga caatgtgtaa tgatatgtta ttcatatggg agatgtgtga aagtgggctt
     9601 tggatgatgg gcaagagaca aaatacccaa gacacctcaa atacaatgta ccaatcatct
     9661 accgtttttt ttttacaaat tattacaaaa gtagaaaaaa cattgatttt ttattgaaag
     9721 ctatttcttt tagataaact gagaagaaag ccgagacaaa tatgaacact agtaatttat
     9781 tttgtttaat gtgtgttaat tgaatttgtt agctttcctt gcgattgcgg gcaaagtgga
     9841 taagagaagt agaagtaggt ttgttctaac tcgtaataaa gtcacagaac taattctaaa
     9901 gaacggtaca tatatcatca ttcataagta cttgatgtct cccattttcg gtgaaacact
     9961 ttacttgacc agtcttttta atatgcaaca aatcttgtat atattcaaca taaaaaaaat
    10021 gcaccatcat tggaattgtt gacccaacaa aaaaacccta taggcattaa aaggctctta
    10081 gagggccaat tttgttatcg ggccttcact tggccagtag aaattacaag catgcaaaca
    10141 acagttcgag acttcgagga agattcctat ctctctctac tcgtttcttg aaccaagtca
    10201 tacaagcaag caagacagct ttattaaagt tgactttact ttaatcttta tgagtcaaca
    10261 cctcatcttt attagttagt tagctcagac aagtaaaagc tgcagcccgc tcgacgaatt
    10321 cggcacgagg acaagtaaaa gaaagagcga gaaatcatcg aaggtaccgg gccccccctc
    10381 gaggcgcgcc aagctatcaa acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
    10441 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
    10501 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
    10561 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
    10621 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
    10681 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
    10741 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
    10801 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
    10861 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
    10921 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
    10981 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
    11041 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
    11101 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
    11161 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
    11221 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
    11281 ggcggggcgt aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg
    11341 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
    11401 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
    11461 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
    11521 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
    11581 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
    11641 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
    11701 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
    11761 cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
    11821 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
    11881 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
    11941 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
    12001 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
    12061 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttcgagc tagcggcggc
    12121 cgcacaggtt cttaccctta tgacgttcct gattatgcat ccggtggcct cgaagttttg
    12181 ttccagggtc cactgggttc cgcagatcca accagctccg aaacatcatc acaccctcca
    12241 ggaaccattg tggcccccat ggctggtttg gtagtcaagg tccttgtcga aaacgaagcc
    12301 aaagtagatc aaggtcaacc tatattagtc ctagaggcaa tgaagatgga gcacgttgtg
    12361 aaagcaccat cctctggaag catacaggac ctcaaagtta aagcaggcca acaggtttca
    12421 gatggcagtg ctctattcag gatcaaaggg taattaatta actagttcta gagcggccgc
    12481 ccaccgcggt ggagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
    12541 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    12601 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    12661 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    12721 gataaattat cgcgcgcggt gtcatctatg ttactgaatt
//
