LOCUS       sequence-36193-e        8142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Feng H, Kwun HJ, Liu X, Gjoerup O, Stolz DB, Chang Y, Moore PS
  TITLE     Cellular and viral factors regulating merkel cell polyomavirus 
            replication.
  JOURNAL   PLoS One. 2011;6(7):e22468. Epub 2011 Jul 22.
  PUBMED    21799863
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22468. Epub 2011 Jul 22"
COMMENT     Sequence Label: MCV-hVam6p-
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(810..901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     968..987
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1194..1227
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(6641..6674)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7144..7161)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7144..7160)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7259..7276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7430..8018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7510..7529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8142,1..809))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
       61 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
      121 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
      181 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
      241 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
      301 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
      361 gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag
      421 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
      481 tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca
      541 tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt
      601 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
      661 aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca
      721 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
      781 ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt
      841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
      901 ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata
      961 cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc
     1021 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc
     1081 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1141 gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc
     1201 ctattctcta gaaagtatag gaacttctga agtggggacc ggctatttag tatcagattc
     1261 actaggtttg attgtatctg cagcctagag gtaggagata aagaattaaa aatatcttgc
     1321 cccacagaat gcagcaagct attttcccac tgcagaggat ctaggctaaa ggccataagt
     1381 gcatgcctca aaacctcatt actacctacc cacgaaacat ccctctttac aagtgacact
     1441 tgctcgcgtg acaacctcac ccccacagtt attagagagc ctataccact aacagtttgg
     1501 agaatgaagc cataagttaa accttggtta accaaagaag ccactaatga gaaatttgaa
     1561 aactgttcag ctgtgaaccc aagttgagct aaagcctcaa tgccagaaat accctcaatt
     1621 gtcattaaac tggagatctc tgcttccaaa gctgctaaag cttctcctgt aagaatagct
     1681 tccaaagtta ctcctgtggt ggcacttagt tcagtagcaa tttcaccaat attggccagc
     1741 agtgtgatga tgccccccat cctgaaaaat aaataaggat acttactctt ttaatgtcct
     1801 cctccctttg taagagaaaa aaaagcctcc gggcctccct tgttgaaaaa aagttaagag
     1861 tcttccgtct ccctcccaaa cagaaagaaa aaaagttttg tttatcagtc aaactccgcc
     1921 tctccaggaa atgagtcaat gccagaaacc ctgcagcaat aaaagttcaa tcatgtaacc
     1981 acaacttggc tgcctaggtg actttttttt ttcaagttgg cagaggcttg gggctcctag
     2041 cctccgaggc ctctggaaaa aaaagagaga ggcctctgag gcttaagagg cttaattagc
     2101 aaaaaaggca gtatctaagg gcagatccca agggcgggaa actgcagtat aaaaaccact
     2161 ccttagtgag gtagctcatt tgctcctctg ctctttctgc aaactccttc tgcatataga
     2221 caagatggat ttagtcctaa ataggaaaga aagagaggct ctctgcaagc ttttagagat
     2281 tgctcctaat tgttatggca acatccctct gatgaaagct gctttcaaaa gaagctgctt
     2341 aaagcatcac cctgataaag ggggaaatcc tgttataatg atggaattga acaccctttg
     2401 gagcaaattc cagcaaaata tccacaagct cagaagtgac ttctctatgt ttgatgaggt
     2461 cagtacaaaa tttccttggg aagaatatgg aactttaaag gattatatgc aaagtggata
     2521 taatgctaga ttttgcagag gtcctgggtg catgcttaag caacttagag attctaagtg
     2581 cgcttgtatt agctgtaagt tgtctcgcca gcattgtagt ctaaaaactt taaagcaaaa
     2641 aaactgtctg acgtggggag agtgtttttg ctatcagtgc tttattcttt ggtttggatt
     2701 tcctcctact tgggaaagtt ttgactggtg gcaaaaaact ttagaagaaa ctgactactg
     2761 cttactgcat ctgcaccttt tctagactcc tacttccttc ctctgtaagt attagatatg
     2821 gaaaagtcta taaggcaaaa tatcaaagaa aggttattta tgacagattt tctgtacttt
     2881 cccatctagg ttgacgaggc ccctatatat gggaccacta aattcaaaga atggtggaga
     2941 tcaggaggat tcagcttcgg gaaggcatac gaatatgggc ccaatccaca cgggaccaac
     3001 tcaagatcca gaaagccttc ctccaatgca tccaggggag cccccagtgg aagctcacca
     3061 ccccacagcc agagctcttc ctctgggtat gggtccttct cagcgtccca ggcttcagac
     3121 tcccagtcca gaggacccga tatacctccc gaacaccatg aggaacccac ctcatcctct
     3181 ggatccagta gcagagagga gaccaccaat tcaggaagag aatccagcac acccaatgga
     3241 accagtgtac ctagaaattc ttccagaacg gatggcaccg cggaggatct cttctgcgat
     3301 gaatcacttt cctcccctga gcctccctcg tcctctgagg agcctgagga gcccccctcc
     3361 tcaagaagct cgccccggca gcccccgtct tcctctgccg aggaggcctc gtcatctcag
     3421 tttacagatg aggaatacag atcctcctcc ttcaccaccc cgaagacccc tcctccattc
     3481 tcaagaaagc gaaaatttgg ggggtcccga agctctgcaa gctctgctag ttcagcaagt
     3541 tttacaagca ctccaccaaa gccaaaaaag aacagagaaa ctcctgttcc tactgatttt
     3601 cctattgatc tttctgatta tcttagccat gctgtatata gtaataaaac agtaagttgt
     3661 tttgccattt atactacttc tgataaagct atagagttat atgataagat tgagaaattt
     3721 aaagttgatt ttaaaagcag gcatgcctgt gaattaggat gtattttatt gtttataact
     3781 ttatcaaagc atagagtatc tgctattaag aatttttgct ctaccttctg cactataagc
     3841 tttttaattt gtaaaggagt gaataagatg cctgaaatgt ataataattt atgcaagccc
     3901 ccttacaaat tactgcaaga gaataagcca ctgctcaatt atgaatttca agaaaaagaa
     3961 aaagaggcca gctgtaattg gaatttagtt gctgaatttg cttgtgaata tgagctagac
     4021 gaccacttta ttatcttagc ccattatcta gactttgcaa aaccatttcc ttgccaaaag
     4081 tgtgaaaaca gatctcgcct caaacctcac aaggctcatg aggctcatca ttctaatgct
     4141 aagctatttt atgaatctaa atctcagaaa accatttgcc aacaagccgc agacactgtt
     4201 ctagccaaaa ggaggttaga gatgctggaa atgaccagga cagaaatgct atgtaagaag
     4261 tttaagaagc acctagagag attaagagat ttagatacaa tagatctact gtattatatg
     4321 ggtggtgtgg cctggtactg ctgcttattt gaagagtttg aaaagaagct gcagaaaatt
     4381 attcaattat taacagagaa tatacctaag tatagaaaca tttggtttaa agggcctatt
     4441 aacagtggaa aaacaagctt tgctgcagcc ttaatagatt tgctagaagg gaaggccttg
     4501 aatataaact gtccatctga taaactgcct tttgaactag gatgtgcttt ggataaattt
     4561 atggttgttt ttgaggatgt gaaagggcaa aatagcctaa ataaagatct gcaaccaggg
     4621 caaggaataa ataaccttga taacttaaga gatcatctag atggtgctgt agctgtaagc
     4681 ttagagaaga agcatgtgaa taaaaagcat cagatttttc ctccttgtat tgttactgct
     4741 aatgattatt ttattcccaa aacattaata gcaagattta gttatacttt acacttttcc
     4801 ccaaaggcaa atctaagaga ttccctggat cagaacatgg aaataagaaa aagaagaatt
     4861 cttcaaagtg gaaccacttt attgctttgt cttatttggt gcttgcctga tacaaccttt
     4921 aagccttgct tacaagaaga aattaaaaac tggaagcaaa ttttacagag tgaaatatca
     4981 tatggtaaat tttgtcaaat gatagaaaat gtagaagctg gtcaggaccc tctgctcaat
     5041 attcttattg aggaagaggg ccctgaggaa actgaagaaa cccaagattc tggtactttt
     5101 tctcaataaa ggcatctgct tcatatttcc tgtgtttgtt tttctggggc ctacttaact
     5161 gaataggaat gcatgaaata attctcataa ttcttgtgtt tggctttctt tttgagaggc
     5221 cttttgaggt cctttcagtg gcgccttgcc cttatcctgc tgattacttt ggaatgttac
     5281 tgctgctggg gcaacagagg gctttgggta aacagttttc tcctgcccaa atttatctaa
     5341 aaatctgaca atatcaggat caccaggtaa ttgttctgac ccctcatata ttctaacctc
     5401 ttctacctga ttatcttttc cttccatagg ttggcctgac acttttggca ttaagttgct
     5461 gaagagtgag tttattaaat taactactgg gtaggggttt ttcacccatc tttttctcaa
     5521 agtaacatta aaatatctag gcaacccatg aagagccatt tttccactgg ttttaaacag
     5581 aaaccccact atgtctgcac agctaataaa taggccatct cctttgcata gagggcccac
     5641 tccattctca tctaaaagga cagtagttag agtattacta aattgaagaa ctgtaggagt
     5701 ctgagagcct gtctgaatag acccatagta tctactgttt tcatttttag aaggatcagg
     5761 acaccatact tctataggat aatttccatc tttatctaat tttgctttag cttgtggatc
     5821 taggccctga tttttaggtg tcatttttct tcccaataca gtttcaattg taataggccc
     5881 accatttgta gtttttggat actcagtctg gtaatctaaa actaggcctt gcaaatccag
     5941 aggttctccc ccaatggcaa acatatggta atttacccct gacacaggaa taccagcacc
     6001 ataatcatga actcttttca tgtcccaata atgaacatta attaaagaac ttattccaac
     6061 tacttctgtt ttaacagata ttgcctccca catctgcaat gtgtcacagg taatatcctc
     6121 atttagcatt ggcagagaca ctcttgccac actgtaagct ggcaaatttt ccttgatggg
     6181 ctgatctgga gatgatccct ttggctgcag gtcataagta taagtatacc agtttgaagt
     6241 agtaggaaga tcaggggaat taactcccat tcttggattc aaatacaact caatttgggt
     6301 aatgctatct tctccagtaa ccacagataa tacttccact cctcctttaa caagcagttt
     6361 tggaactgag gcaacattag ggcagcatcc cggcttaggt atacattgcc ttttgggtgt
     6421 tttacaggtg gatgatgctt ttctttttgg tgccatcttc aattacttgt aattcaggag
     6481 aaatatatcc actaaggcct agtaccagag gaagaagcca atctggagtt tgctgctgca
     6541 gagttcctcc tatatgttca ggaattaata tagcctctcc tgataaaagg ccctgattct
     6601 gagaagcagt tgtctgaaag acccaccggc aaaatgaagt gaagttccta tactttctag
     6661 agaataggaa cttctatagt gagtcgaata agggcgacac aaaatttatt ctaaatgcat
     6721 aataaatact gataacatct tatagtttgt attatatttt gtattatcgt tgacatgtat
     6781 aattttgata tcaaaaactg attttccctt tattattttc gagatttatt ttcttaattc
     6841 tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga tgaatagttt
     6901 aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt
     6961 tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc gcccttaaat
     7021 attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt
     7081 ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg gcccgagctt
     7141 aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa aggccatccg
     7201 tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct tccgcttcct
     7261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct
     7861 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8041 cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat
     8101 gagcttgcgc cgtcccgtca agtcagcgta atgctctgct tt
//
