LOCUS       sequence-361958-        8440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression of mouse PKC Delta with yeast PKC1 promoter and 
            ADH1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8440)
  TITLE     Yeast expression plasmids of mouse PKC isoforms with yeast PKC1 
            promoter and ADH1 terminator
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: isoform PKC Delta (JCE594)
FEATURES             Location/Qualifiers
     source          1..8440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..49)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(203..791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(283..302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(962..1753,1754..1822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1585..1604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1823..1927)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1995..2013
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2038..2253
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2254..3057
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    3392..4554
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      3392..4229
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     primer_bind     5296..5318
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5310..5327
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5311..5327
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      7994..8181
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8049..8073)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8241..8257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8241..8257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8254..8276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8265..8281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8289..8295,8296..8313,8314..8319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8334..8355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
       61 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
      961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     1801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     1861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1921 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     1981 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttttc
     2041 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     2101 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     2161 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     2221 agaacaaaaa catgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     2281 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     2341 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     2401 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     2461 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     2521 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     2581 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     2641 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     2701 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     2761 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     2821 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     2881 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     2941 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     3001 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     3061 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     3121 tatcagttat tacccaattc tcatgtttga cagcttatca tcgatcgtcc aactgcatgg
     3181 agatgagtcg tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt
     3241 atttccaaaa gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat
     3301 tcccttgttt gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc
     3361 tgactgggtt ggaaggcaag agagccccga gagcttacat tttatgttag ctggtggact
     3421 gacgccagaa aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag
     3481 cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa
     3541 tgctaagaaa taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgcct
     3601 gcaagccttt tgaaaagcaa gcataaaaga tctaaacata aaatctgtaa aataacaaga
     3661 tgtaaagata atgctaaatc atttggcttt ttgattgatt gtacaggaaa atatacatcg
     3721 cagggggttg acttttacca tttcaccgca atggaatcaa acttgttgaa gagaatgttc
     3781 acaggcgcat acgctacaat gacccgattc ttgctagcct tttctcggtc ttgcaaacaa
     3841 ccgccggcag cttagtatat aaatacacat gtacatacct ctctccgtat cctcgtaatc
     3901 attttcttgt atttatcgtc ttttcgctgt aaaaacttta tcacacttat ctcaaataca
     3961 cttattaacc gcttttacta ttatcttcta cgctgacagt aatatcaaac agtgacacat
     4021 attaaacaca gtggtttctt tgcataaaca ccatcagcct caagtcgtca agtaaagatt
     4081 tcgtgttcat gcagatagat aacaatctat atgttgataa ttagcgttgc ctcatcaatg
     4141 cgagatccgt ttaaccggac cctagtgcac ttaccccacg ttcggtccac tgtgtgccga
     4201 acatgctcct tcactatttt aacatgtgga attaattctc atgtttgaca gcttatcatc
     4261 gaactctaag aggtgatact tatttactgt aaaactgtga cgataaaacc ggaaggaaga
     4321 ataagaaaac tcgaactgat ctataatgcc tattttctgt aaagagttta agctatgaaa
     4381 gcctcggcat tttggccgct cctaggtagt gctttttttc caaggacaaa acagtttctt
     4441 tttcttgagc aggttttatg tttcggtaat cataaacaat aaataaatta tttcatttat
     4501 gtttaaaaat aaaaaataaa aaagtatttt aaatttttaa aaaagttgat tataagcatg
     4561 tgaccttttg caagcaatta aattttgcaa tttgtgattt aggcaaaagt tactatttct
     4621 ggctcgtgta atatatgtat gctaatgtga acttttacaa agtcgatatg gacttagtca
     4681 aaagaaattt tcttaaaaat atatagcact agccaattta gcacttcttt atgagatata
     4741 ttatagactt tattaagcca gatttgtgta ttatatgtat ttacccggcg aatcatggac
     4801 atacattctg aaataggtaa tattctctat ggtgagacag catagataac ctaggataca
     4861 agttaaaagc tagtactgtt ttgcagtaat ttttttcttt tttataagaa tgttaccacc
     4921 taaataagtt ataaagtcaa tagttaagtt tgatatttga ttgtaaaata ccgtaatata
     4981 tttgcatgat caaaaggctc aatgttgact agccagcatg tcaaccacta tattgatcac
     5041 cgatattagg acttccacac caactagtaa tatgacaata aattcaagat attcttcatg
     5101 agaatggccc agcgattatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5161 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5221 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     5281 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagtggt
     5341 tcagcttctg tttgagttgc aaccatggta tgcatgccgg tcacgctgaa gaatggtttg
     5401 acagacataa tgtttgtccc actccaggtt gcacctgcca gtgtaataag tagtattgta
     5461 caataaacca aatcttgcaa cttctcttta ttctcacaat atatatacat aaccaaagaa
     5521 aaaacgtaat tagtataaca ttgtatttat gaggcgttgc tatcttcatt acccagcccg
     5581 cctagtttta aaggcgaaaa aaaccgacga aaagagttaa caagcgtgga taataaagtt
     5641 gatctactct atcgaaagta atacgacagg ttcaggagca gtaaaagagc tacgatttca
     5701 ctgctattca atttggctga gtagagactg attattaacc attgtaaaag tctctgccac
     5761 cactcgtcca cacttacgtt agaggtattc agcgtcgatc ccctttcagt atagattttt
     5821 aaaactatat tagataatca ataggaataa aacctagaac agtagctgcg gagagaagct
     5881 ctagcgagga aaagtaagta tagtatcaca catataggga gcagtttaca gtcggtacca
     5941 tggcaccctt cctgcgcatc tccttcaatt cctatgagct gggctccctg caagttgagg
     6001 acgaagcaag ccagcctttc tgtgctgtga agatgaagga ggcactcagc acagagcgag
     6061 ggaagacact ggtacagaag aagcccacca tgtatcctga gtggaagaca acgttcgacg
     6121 cccacatcta tgaaggccgt gttatccaga ttgtgctgat gcgggcagct gaagaccccg
     6181 tgtctgaggt cacggtgggc gtgtcagtac tggctgagcg ctgcaagaag aacaacggca
     6241 aggctgagtt ctggctggat ctgcagcctc aggccaaggt gctgatgtgt gtgcagtatt
     6301 tcctggagga tggggattgc aaacagtcta tgcgtagtga ggaggaggca aagtttccaa
     6361 ccatgaaccg tcgtggagcc attaaacagg ccaagatcca ctacatcaag aaccacgagt
     6421 ttatcgccac cttcttcggg cagcccacct tctgttctgt gtgcaaagag tttgtctggg
     6481 gcctcaacaa gcaaggctac aaatgcaggc aatgcaacgc tgccatccac aagaaatgca
     6541 ttgacaagat tatcggccgc tgcactggca ctgccaccaa tagccgggac accatcttcc
     6601 agaaagaacg cttcaacatc gacatgcctc accgattcaa ggtttataac tacatgagcc
     6661 ccaccttctg tgaccactgt ggcagtttgc tctggggact ggtgaagcag ggattaaagt
     6721 gtgaagattg tggcatgaat gtgcaccaca aatgccggga gaaggtggcc aacctgtgtg
     6781 gtatcaacca aaagctcttg gctgaggcct tgaaccaagt gacccagaga tcttcccgga
     6841 agctggacac aacagagtct gtcggaatat accagggatt tgagaagaag ccagaagtct
     6901 ctgggagtga catcctagac aacaacggga cctatggcaa gatctgggag gggagcaccc
     6961 ggtgcaccct tgagaacttc accttccaaa aagtacttgg caaaggcagc tttggcaagg
     7021 tgctgctggc agagctgaag ggcaaagaca agtactttgc aatcaagtgt ctgaagaagg
     7081 acgtggtgtt gattgacgat gatgtagagt gtaccatggt ggagaagcgg gtgctggcgc
     7141 tcgcctggga gagtcccttc ctcacccacc tcatctgtac cttccagacc aaggaccacc
     7201 tgttcttcgt gatggagttt ctcaatgggg gtgacctgat gttccacatt caggacaaag
     7261 gccgcttcga actctaccgg gctacgtttt atgcagctga gatcatctgc ggactgcagt
     7321 ttctacacag caaaggcatt atttacaggg acctcaagct ggacaatgtg atgctagaca
     7381 gggacggcca catcaagatc gctgactttg ggatgtgcaa agagaatata tttggggagg
     7441 gccgggccag cacattctgc ggcactcctg actacatcgc ccctgagatc ctgcagggcc
     7501 tgaagtactc cttctcggtg gactggtggt ctttcggggt cctcctgtac gaaatgctca
     7561 tcggccagtc ccccttccac ggcgacgatg aggacgagct cttcgagtcc atccgggtgg
     7621 acacaccaca ctatccccgt tggatcacca aggaatccaa ggacatcatg gagaagctat
     7681 tcgagaggga ccctgacaag aggctgggag taacaggaaa catcaggatt cacccctttt
     7741 tcaagactat caactggtcc ctcctggaga agcggaaggt ggagccgccc tttaagccca
     7801 aagtgaaatc cccttcagac tacagcaact ttgacccaga gttcctgaat gagaaacctc
     7861 agctttcctt cagtgacaag aacctcatcg actctatgga ccaggaagcc ttccatggct
     7921 tctcctttgt gaatcccaag tttgagcaat tcctggacat ttaagtcgac tagggcgcgc
     7981 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     8041 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     8101 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct
     8161 tattgaccac acctctaccg gcagatccgc tagggataac agggtaatat ctgcaggcat
     8221 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8281 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8341 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8401 tgccagctgc attaatgaat cggccaacgc gcggggagag
//
