LOCUS       sequence-36197-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer 
            AL, Baserga SJ
  TITLE     RNA polymerase I transcription and pre-rRNA processing are linked by
            specific SSU processome components.
  JOURNAL   Genes Dev. 2004 Oct 15. 18(20):2506-17.
  PUBMED    15489292
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Oct 15. 18(20):2506-17."
COMMENT     Sequence Label: pTOPO-rRNA-5'ETS 347 to 631
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(399..418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(400..418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(425..442)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(425..441)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(434..456)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(669..688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     879..900
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcggatcca ctagtaacgg ccgccagtgt gctggaattc gcccttgaat agccggtcgc
       61 aagactgtga ttcttcaagt gtaacctcct ctcaaatcag cgatatcaaa cgtaccattc
      121 cgtgaaacac cggggtatct gtttggtgga acctgattag aggaaactca aagaatgcta
      181 tggtatggtg acggagtgcg ctggtcaaga gtgtaaaagc tttttgaaca gagagcattt
      241 ccggcagcag agagacctga aaaagcaatt tttctggaat ttcagctgtt tccaaactca
      301 ataagtatct tctagcaaga gggaatagga agggcgaatt ctgcagatat ccatcacact
      361 ggcggccgct cgagcatgca tctagagggc ccaattcgcc ctatagtgag tcgtattaca
      421 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      481 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      541 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
      601 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      661 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      721 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      781 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      841 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      901 aacaacactc aaccctatct cggtctat
//
