LOCUS       sequence-362-exp         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Shen S, Arhin GK, Ullu E, Tschudi C
  TITLE     In vivo epitope tagging of Trypanosoma brucei genes using a one step
            PCR-based strategy.
  JOURNAL   Mol Biochem Parasitol 2001 Mar;113(1):171-3.
  PUBMED    11254965
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biochem
            Parasitol"; date: "2001-03"; volume: "113(1)"; pages: "171-3"
COMMENT     Sequence Label: Phleo/Xpress
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVXGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             join(899..907,908..922)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
       61 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
      121 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
      181 aacaccctgg cctgggtgtg ggtgcncggc ctggacgagc tgtacgccga gtggtcggag
      241 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
      301 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
      361 gaggagcagg actgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
      421 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
      481 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
      541 cccaccccaa ttcgagctcg gtacccctag aaagtgtgac aacgtcgcac catgtgtagg
      601 ttttcattta tgttctttct ttctttcttt tgtgaatttg ttttctgtct caaatgtttt
      661 taattcgctt gggacctatg tttttcttgt ttttttgctc accctttgtg taggaggcac
      721 cctgtcacgt ctgtggttgc gtgtatgcct tccttcccct tattcgcttc ttcctgtcgt
      781 gtcacacctc tttctccctc tccctttccg ccttttcttt caatcttgtt ttctcgacca
      841 gccctactag aggagaaaga atagtaaccc tttcatcaaa tagtccaaac gaattatgga
      901 tctgtacgac gatgacgata ag
//
