LOCUS       sequence-362024-       11443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, 
            alpha-cry:EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Hong J, Lee JG, Sohn KC, Lee K, Lee S, Lee J, Hong J, Choi D, Hong 
            Y, Jin HS, Choi DK, Lee SU, Kee Y, Jung J, Bae YK, Hwang RH, Hur GM,
            Lee JS, Ro H
  TITLE     IQ-Switch is a QF-based innocuous, silencing-free, and inducible 
            gene switch system in zebrafish.
  JOURNAL   Commun Biol. 2021 Dec 16;4(1):1405. doi: 10.1038/s42003-021-02923-3.
  PUBMED    34916605
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-16"; volume: "4(1):1405. doi"; pages: " 
            10.1038/s42003-021-02923-3"
COMMENT     Sequence Label: pubb-QFDBD-2x AD*-VP16*-EcR, alpha-cry:EGFP
FEATURES             Location/Qualifiers
     source          1..11443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(659..670,671..808)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     810..826
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     811..827
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      8047..8056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8053..8055,8056..8058,8059..8772)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8053..8055,8056..8058,8059..8769)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8098..8119)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8359..8378)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8706..8727
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8899..9020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8936..8955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8990..9009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(9037..9224,9225..9236)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9255..9275)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9255..9273)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9294..9310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9294..9310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9307..9329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9318..9334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9342..9348,9349..9366,9367..9372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9387..9408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9525..9542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9696..10284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9776..9795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10455..11246,11247..11315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11078..11097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11316..11420)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccca
      661 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
      721 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
      781 tactccatta aaattgtact tgagtattct cgaggtcgac ggtatcgata agcttgatat
      841 cgaattacca gcaaagttct agaatttgtc gaaacattta tgttatatat ttcctgaaaa
      901 aaattctgag taagttctta agtgttattg ccagcaacat aaacaacaga cggcaaaatg
      961 aataaatgat aacaaagcag taggcttaaa taaacctaat ttttataggc tgttctctac
     1021 aaccctcaaa cagtgattag ttttgtactt ataaacttgc cctttcattc atatttcaag
     1081 aaaattggtt cagaagatct ggatattcta gcagttgttc aagctcatgg agggatcagt
     1141 gacctgattc cacaatgact aggcctaatc cagaaattag atgactgtca acataaaaag
     1201 gcacagcact cactagctgc cctatatatt ttattatatt ttacatatat tattttattt
     1261 atttagctct gagtgctgta ctttctggtt aaagaaaact gcttacaaca gctaacctgt
     1321 actacctcag gctcagggaa tttggaacag gtttgtctgg tttgtttctt taaccatgca
     1381 tgcttgtttt caactatggc aacacagtca catgggacat tacggaaatg atttgtcgat
     1441 gacatgcgac ttttctttaa taaagcgcaa agatcccaaa aagcaaactt ttaacaaaaa
     1501 tcatataatt atattttcaa tccagctttg tagcaacttt gtgctgctgt tcactcagca
     1561 acagatagtc agtataaggt cagtgtgtct caaagcagtg ccatctgttt cacacattgc
     1621 gttctatata taagtgtgct ggttgacacg acactgtata aggcctaggc taaaacacaa
     1681 acaatgtaga atgacactgt gttttttttg taaacaaatg ttgtttttgg ttaaacatct
     1741 ttgtgaaaac atcctcctgt catgtatttg ctatattcaa atgttaaacc cgtgcagaat
     1801 agaacatata caaaaaaaaa caacacaaca catttttaaa cattattaaa tatcaagtat
     1861 tgctggcagt tctgtttctg ttttacagta ccctttgcca cagttctccg cttttcctgg
     1921 tccagattcc acaagtctga ttcaccaata gcaaagcgaa taaacaacca aagcagccaa
     1981 tcactgcttg tagactgtcc tgcgagaccg gcccattcca gcacattctg gaaacttcct
     2041 ttatatgata attataaata catttaaatt attgatacaa aacatgtaat tcctagaaca
     2101 taaccatagc aatcattagt tttcagggta attatgtatt tttaggattt gactgcggaa
     2161 agatctggtc atgtgacgtc tcatgaacgt cacggccctg ggtttctata aatacagtag
     2221 gactctcgac catcggcaga tttttcgaag aagaagatca gtttcaggag ccgtgctgtt
     2281 ccgtttcaac gcaaatatta acggtaagag cgaatttcct agtttgtttt catgccattc
     2341 tttaaaacca tagcgtatta ctttaattat agtaaacttt cgctttcttt attacaagag
     2401 acgttttgtg ttgattctcc gcggacattt tcggtcagac aatcagaaaa tgaccgcgga
     2461 ggaccagtaa cttgcattac acgtaagtta aatcttcgtg tattaaaatg gttaggttgt
     2521 taacgtcaaa taggttgccg tgtttgcgtg tgatcaggtt ggttttgtta gatttttgtc
     2581 agtattttta atttatttgt tttagtttat ttattttttt tgctgaatca tagtttgtga
     2641 acaaagaacc cggatgttac atacagtaca gccgccatgt tacagagagt tataacttaa
     2701 tcattttaaa aataattttg ccttactttt agtttgtcat gttgagaaat gaggaaatgt
     2761 taaaatgagg aaatatccaa ttaatttaat atatcaaaat aatccatgat tacaatgcac
     2821 tgaactggag aaaattaaga tgttttctag tgtcatgaaa caaatgtaag agatgtacat
     2881 tgtagatgtt ttatgtcaag aattggctag ttgatgcagc atactggcga tactcagttg
     2941 taataacagt aacgttacat gttaatagac tactgagtat gctgttctgt ctatgtatgc
     3001 tctgtaagct acgagaagga cttttttaaa cagtaaaggg tgcaatattt ttacaaattg
     3061 aattaaataa aggctgtcta ttaagtaata tgcttgatat ttttcttact tgatcggaaa
     3121 taagaaaaaa tataaacgtt gttgctctaa aaatcctagt tcagtttagc caaccacaaa
     3181 tacctttttg ttcctccaac agtttttttt tcttctctat aatatttggc agtctatagt
     3241 actccaaatg tttccccaca gtctaactaa ttggtacagc caaaatcatg acacttattg
     3301 caataataat tttggttcat tggcattgtt gatagcctgt gccactaata tggtcgattg
     3361 atcatgcttc aggaagaaaa ctatattgtt tgatgtaaga ttattaaatc ttcacctgct
     3421 tccattacaa actattccca tcttattgaa ttctggtatg tcttaaagga ttagttcact
     3481 tcccaaatca aaatttactt agcctttttt catccatgat ccctttttca tcattaatga
     3541 agaaattgtt tttgaaaaag tttcaagatt tttttcccta tattgtggag cttgttagtt
     3601 taaaattcca aaatgcaata tgtggcttca aatggttcta aatgatccca gtcaaggaat
     3661 aacagtctta tctaatgaaa ccattagacc tttaaaaata aaaaaaataa aagtatttat
     3721 ttttaaatga ctgagtgatt aagttgaatt tcagcgtttc cttactgtgt agaagtcctt
     3781 ccttactggc cccacccttt ggttctctgc caatctgcta cctaatgtaa tgttgtggaa
     3841 cattattatt ctttatttct taatttttta ttttttattt taaaacaatg taaactgcac
     3901 agatgtgcag tttgtttaaa atggccaatg ctttggaaat gcatgacata attagatttc
     3961 atgatgcaca aagccaaatc tcagagcttg tgcaaaatga gctatcattt cactaggtaa
     4021 gaccctaaat tttcatatag gatcatttgg acaattttgc tgcaggtaaa atgcattcta
     4081 tagtccactg tcagccattg ttttggatag tatttatttt tctctacgag tatagtcaat
     4141 agttttctat tattttaaag gtttgtaaca tttaagggtg accaaatgca aagtaaaatt
     4201 tcattttcgg gtgaactatc tcgtttaaca tgggagaagt gcaaaacata cattattggc
     4261 tagaacattg tagtattttt taaatggaaa tgtgtgattg ctaatcttac tttgaatttg
     4321 tttacaggaa ttcctgcagc ccggggccaa catgccgcct aaacgcaaga cactcaatgc
     4381 cgctgccgaa gccaatgccc acgctgatgg ccatgctgat ggcaatgctg atggtcatgt
     4441 cgctaacact gcagcaagca gcaacaacgc ccgttttgcg gacttgacca acattgacac
     4501 acccggcctc ggccctacca cgacgacgtt acttgtcgag cccgctcgtt cgaaacgcca
     4561 gagagtctcg agggcctgtg atcagtgtcg agctgcacgt gaaaagtgtg atggaatcca
     4621 gccggcttgc ttcccctgtg tgtcgcaggg ccggtcgtgt acctaccagg ccagtcccaa
     4681 gaagcgagga gtccagacgg gctacatccg cactctcgaa ctggctctgg cttggatgtt
     4741 cgagaacgtt gcccgcagcg aggacgccct ccacaatctt ttggtccgtg atgctggcca
     4801 gggcagcgct ctcctggtcg gcaaagactc gcctgctgca gaacgcctgc atgcaagatg
     4861 ggcgacgagt cgagtcaaca aaagcatcac ccgtcttctc tcaggtcagg ccgcacaaga
     4921 tccatctgaa gacggccaat ccccgtccga agacataaat gtccaagatc aggccgcaca
     4981 agatccatct gaagacggcc aatccccgtc cgaagacata aatgtccaag atgagttcct
     5041 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     5101 cggtgggctg cagatgaggc ctgaatgtgt catacaggag cccagtaaaa ataaagacag
     5161 gcaaagacaa aagaaagaca aaggaatatt attacctgtt agtacgacca cagtcgaaga
     5221 ccacatgccc ccgatcatgc aatgtgatcc acctccgccc gaggccgcca ggattcacga
     5281 agtcgtcccg aggtatcttt cggagaagct gatggagcag aacaggcaga agaacatacc
     5341 accattgtcg gcgaatcaga agtctctgat cgcgaggctc gtgtggtacc aggagggata
     5401 tgagcagccc tccgacgagg atctcaaaag agtaacgcag acttggcagt cggatgaaga
     5461 ggacgaggaa tccgatctac ccttccgcca gatcacggag atgacgatct taacggtcca
     5521 gttgatcgtc gagttcgcca agggtctacc gggcttttcg aagatatcac agtctgatca
     5581 aatcacctta ttaaaagcct cgtccagcga ggtgatgatg ctgcgggtgg cgaggcgata
     5641 cgacgccgcg tccgacagca ttctgttcgc caacaacaag gcgtacacgc gcgacaacta
     5701 ccgcaaggcg ggcatggccg aggtcatcga agacctccta cacttctgcc ggtgcatgtt
     5761 cgcgatgggc atggacaatg tgcactttgc actgctcacg gccatcgtta tattctcaga
     5821 tcggcccggg ctcgagcagc cgtcgctggt agaagagatc cagagatact acctgaacac
     5881 gttgcgaatt tacatcatca accagaacag cgcgtcgtcg cgctgcgccg tgatctacgg
     5941 caggatcctg agcgtgctga ccgagctacg cacgctcggc acgcaaaact ccaacatgtg
     6001 catctcgctg aagctgaaga acaggaagct gccgccgttc ctcgaggaga tctgggacgt
     6061 ggcggaggtg gccacgacgc atcccacggt gctgccgccc accaacccgg tggtgctata
     6121 ggggggatcc actagtaatt aagtctcagc caccgttaac tgaacatgtc aaaacctgtg
     6181 gagactgttg agatttgatg ttctgaaaag ataaagccta taaataaaat gttgcccaaa
     6241 tttcctgcct gatgtttttc tttgtctttg ctacatggct ttgctgctcg gatcggctca
     6301 ctctgtgtat gccacgttca ctttgtactc tccttctcac ggtaggttta ttatttttag
     6361 atgtgcagtt agtttctgtg aaataacaca ccacacactg atattgtctg tgcattgact
     6421 tggtgagtgc acattgtttt tgatcttgac atatttatat ttgattgatc aggtgaactg
     6481 tgtgaatcta aagtgctcca tacagatgtt ctgcattgaa aatattctca ttttattagt
     6541 ggaagtgagt gtatgccaca tccaatcaat ttcagcaaac accccagtat gatttaatgc
     6601 aaaaaaatga aggtatcaaa cacgcattac tactttgcag ttaaatattt aacatttatt
     6661 ccaacacgaa aaaaagcagt aaataacact ttgacaaaca cgtcaggaca tcttattttt
     6721 gtcaccctca caggcaattt agtataatat attatatata tatatatatc atataataat
     6781 attcagtata atatatatat atatatcata ttataatatt cagtataata taaaacacaa
     6841 acacatatat gtataatata atataacatt tttatttatt gagatgcctc tatggaccgt
     6901 gttataagaa gtaaagatca ggagaagtaa acatgaagtg taattatgaa tactgatgtt
     6961 aaattaagct atgatgagtt ttcactgtta atttaccatc tcaattaaat gttgatgcct
     7021 ccatgaccaa gttaagcaga tgagactgag gacaactgta gaagacaaga tgttcacttt
     7081 gctgaatata gctggcttga cagttatcta tgactctata aatatatata tatttttttt
     7141 tttataaaat gatttattta taactatata tccatttctc agacaggtgc ttcatatccc
     7201 tcactcccgt agctgtccat gctggatctg tccccgttgt ttttaaaaag ctaaataagt
     7261 tattaacatg actgcatcca gcgagccaaa cctgtctggt gtacagctac cagagaagct
     7321 tgagatccta gtcaccggcg gccgctaata tggcaataaa cgatcttcag agaaacttgc
     7381 attggtaaat agtttcagcc tatgcatatg tagttattca aactgtaatg actatcttac
     7441 agtattatat gtttataata gtttgatcca ataaatccag atttatatgc ttttcaacat
     7501 aaaattgtga aatgcacaga atgtacatat acaatacatt tcctacaata atgctctctt
     7561 ttctattaat agtgtgcatt cagtgcagga tgttatgtat ttattttctg tagtgcaaac
     7621 tttttcaaag aattttccag tctaagcttt tcagtcaaga aaaaaaaagc gattgattat
     7681 ctgtgatatt taaaaaaatt agctcttaaa tctggtgaac caagtggcca caacacatta
     7741 aatactgacg ttctacagcc atgtagcttc atctggtctg gtttgttttg gcaggcactt
     7801 attttccggt gatagtcggc cacaagagta ttgtctgagc cattcagtgc tagactgtca
     7861 ttctcaggtc agaatccacc ccactgattc tgctgacaac actgttccca ccatgagata
     7921 atgccattcc agagagatcc atttgtaagc ccctctttct gcagcacagg tatataacca
     7981 ggggtctgcc tccactaagg ccggcacaca tcatttgggg atctttgtac tgtggatcca
     8041 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     8101 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     8161 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     8221 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     8281 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     8341 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     8401 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     8461 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     8521 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     8581 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     8641 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     8701 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     8761 ctgtacaagt aaagcggccg gccgcgactc tagatcataa tcagccatac cacatttgta
     8821 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     8881 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9001 aaactcatca atgtatctta agccgcggtg gagctcaagt gatctccaaa aaataagtac
     9061 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     9121 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     9181 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggagc
     9241 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     9301 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9361 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9421 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9481 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10321 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10381 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10561 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10921 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10981 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11041 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11101 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11161 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11221 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11281 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
