LOCUS       sequence-362041-        7805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells 
            expressing Cre but not Flp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7805)
  TITLE     Deisseroth Lab Plasmids
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-nEF Con/Foff DREADD Gq-mCherry
FEATURES             Location/Qualifiers
     source          1..7805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        177..388
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             401..669
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    697..730
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(1764..1797)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(1848..1881)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     2080..2098
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2240..2249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3223..3256
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    3307..3340
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     3486..3505
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3790..3823)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    3932..4520
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3985..4005)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4403..4414)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4552..5028
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4751..4770)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5068..5208
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5068..5197
                     /label=AAV2 ITR
     rep_origin      5283..5738
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5370..5389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5580..5601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5755..5774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5874..5896
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6020..6124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6125..6193,6194..6985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6343..6362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7156..7744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7645..7664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaaggat ctgcgatcgc tccggtgccc gtcagtgggc
      181 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
      241 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      301 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      361 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
      421 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
      481 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
      541 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
      601 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
      661 ttacagatcc aagctgtgac cggcgcctac ggatccgaag ttcctattct tcaaatagta
      721 taggaacttc gcagagtagt agctggattg tagctgctat tagcaatcta aaacctctta
      781 gaagttccta ttcttcaaaa ggtataggaa cttcggcgcg ccgccaccat gaccttgcac
      841 aataacagta caacctcgcc tttgtttcca aacatcagct cctcctggat acacagcccc
      901 tccgatgcag ggctgccccc gggaaccgtc actcatttcg gcagctacaa tgtttctcga
      961 gcagctggca atttctcctc tccagacggt accaccgatg accctctggg aggtcatacc
     1021 gtctggcaag tggtcttcat cgctttctta acgggcatcc tggccttggt gaccatcatc
     1081 ggcaacatcc tggtaattgt gtcatttaag gtcaacaagc agctgaagac ggtcaacaac
     1141 tacttcctct taagcctggc ctgtgccgat ctgattatcg gggtcatttc aatgaatctg
     1201 tttacgacct acatcatcat gaatcgatgg gccttaggga acttggcctg tgacctctgg
     1261 cttgccattg actgcgtagc cagcaatgcc tctgttatga atcttctggt catcagcttt
     1321 gacagatact tttccatcac gaggccgctc acgtaccgag ccaaacgaac aacaaagaga
     1381 gccggtgtga tgatcggtct ggcttgggtc atctcctttg tcctttgggc tcctgccatc
     1441 ttgttctggc aatactttgt tggaaagaga actgtgcctc cgggagagtg cttcattcag
     1501 ttcctcagtg agcccaccat tacttttggc acagccatcg ctggttttta tatgcctgtc
     1561 accattatga ctattttata ctggaggatc tataaggaaa ctgaaaagcg taccaaagag
     1621 cttgctggcc tgcaagcctc tgggacagag gcagagacag aaaactttgt ccaccccacg
     1681 ggcagttctc gaagctgcag cagttacgaa cttcaacagc aaagcatgaa acgctccaac
     1741 aggtaagtgt cggggttgct agcataactt cgtatagtat accttatacg aagttattct
     1801 ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaaata acttcgtata
     1861 ggatacttta tacgaagtta tggtacccct cctgtactca cctgggtcac ggtcaccacg
     1921 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
     1981 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
     2041 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
     2101 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
     2161 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
     2221 tcctcctcgc ccttgctcac catggtggcg accgggggat ccttcaaggc ctgctcgggt
     2281 gcgcgcttgt gaaaaatgac cgactgtctc tgctggtact gctgcttgcg cctctttttt
     2341 ttgtcacact ggcacagcag cagcatcttg aaagtggttc tgaatgtttt gttgcacaga
     2401 gcatagcaca cggggttcac ggtgctgttg atgtagcaca gccagtagcc cagattccaa
     2461 aaggttttgg gtatgcagct gtcacaaaag gtgttcacca gaaccatgat gttgtatggg
     2521 gtccaagtga tgatgaaggc aagcaagatc gcactgaggg tctgggccgc tttcttctcc
     2581 ttgaccaggg acatcctttt ccgcttagtg atctgacttc tggtcttcag agcaaacctc
     2641 ttggccagag tggcttcctt gaaggacaga ggtagagtgg ccgtgctctt acccactgag
     2701 gagttgacgt cagaagtctt agctgtgtcc acggctgact ctagctggat gggaagcttg
     2761 gagaagcttt ttggaaaact gcctccatcg tccacgctct tctgggcctg cagcttgtcg
     2821 gctttcctct ccaagtccac catccccagc tcctcctcag gcacctgcag gttgtccgat
     2881 gagggtaact tggtggagtt gaggatggtg ctgtgacccg gaagcttgag cacgatggag
     2941 tagatggctc tcgtctcgga gccaatgtcc tcctcgtcgg aggaggcgga gttctccagg
     3001 gaggcagcag catcattgtt gttccaactg tcactgctgc tgtggtcttg gtccatctgc
     3061 tcggagctgg gtttccagct cttggttgtg aaccagaagt ggcagcggcc atacttcctc
     3121 ctaaacacat gagaaataga gtcataagca catgggtcac atacaggaga tatgtaataa
     3181 cattaataca attttataaa aaaaaggggg ggcacaacta gtataacttc gtataaggta
     3241 tactatacga agttattaag aggtttcata ttgctaatag cagctacaat ccagctacca
     3301 ttctgcataa cttcgtataa agtatcctat acgaagttat gtcgactgga gagtggccag
     3361 cccttctcat gttcagagaa catggttaac tggttaagtc atgtcgtccc acaggactcc
     3421 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3481 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3541 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3601 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3661 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3721 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3781 taagagctcg aagttcctat actatttgaa gaataggaac ttcttgcctt aacccagaaa
     3841 ttatcactgt tattctttag aatggtgcaa agagaagttc ctataccttt tgaagaatag
     3901 gaacttcgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3961 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4021 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4081 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4141 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4201 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4261 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4321 ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg
     4381 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4441 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4501 cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat
     4561 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     4621 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     4681 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     4741 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     4801 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     4861 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     4921 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     4981 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag
     5041 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     5101 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     5161 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     5221 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     5281 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5341 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5401 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5461 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     5521 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5581 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     5641 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     5701 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     5761 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5821 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5881 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5941 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6001 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6061 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7801 catgt
//
