LOCUS       sequence-362062-        3455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to see protein dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Poon J, Fries A, Wessel GM, Yajima M
  TITLE     Evolutionary modification of AGS protein contributes to formation of
            micromeres in sea urchins.
  JOURNAL   Nat Commun. 2019 Aug 22;10(1):3779. doi: 10.1038/s41467-019-11560-8.
  PUBMED    31439829
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-22"; volume: "10(1):3779. doi"; pages: " 
            10.1038/s41467-019-11560-8"
COMMENT     Sequence Label: SpPLK1L-GFP
FEATURES             Location/Qualifiers
     source          1..3455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     194..213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     427..446
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     736..753
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           766..808
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(876..897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1137..1156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1484..1505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     3'UTR           3173..3298
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3397..3413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3397..3413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3410..3432)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3421..3437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attaacctat aaaaaatagg cgtatcacga ggcccttttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acntntgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcttgt tctttttgca
      781 gaagctcaga ataaacgctc aactttggca gatctatgcc atcggggccc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct atacaagtcc ggaggggatc
     1561 ggtggagctc caccggtggc ggccgcatgt cgctacaggg tggttttgca aagtgttatg
     1621 agctgactga cgatgccacc aaacagatct ttgccggaaa ggtcgtctcc aaggccctac
     1681 ttgtcaaacc tcaccaaaaa gacaagatga caatggagat tcacattcat aagagcctgc
     1741 atcacaggca cgtcgttggc ttccatagtt tctttgaaga caaggaaaac gtctacgtcc
     1801 tactggagct gtgcaggaga aggtcactaa tggagctgca caagagacga aaggccatca
     1861 ctgagcccga gacgcgctac ttcatgcgtc agtgcatcct ggcctgtcag tatctctcca
     1921 agaccaaggt cattcaccgt gacctcaagc tgggtaacct cttcatcgac gacaacatgg
     1981 agctcaaggt cggggacttt ggtctcgcca ccaaggtcga cttcagcgga gaacgcaaga
     2041 agacactgtg tggaactcct aactacattg ccccggaagt gctttccaag aaaggtcaca
     2101 gctacgaggt tgacctttgg tcactaggat gcatcatgta caccctcttg gtggggaaac
     2161 ccccttttga aactcagagt ctgaaagaca cctaccagcg aatcaagaga aatgaataca
     2221 gagtaccatc ccatgtcagt acaccggctc gcaatctcat tgttaaatta ctcaagaacg
     2281 accctacgca gcgacctcac atcgacatcc tcctgcaaga cgagttcttc accacagggt
     2341 acctgccccc tcagctcccc accacctgtc tcaccatggc tcctaggttc caagtgcctg
     2401 tatcgtcagg cagaagacca ctcctagagg tcaacggcca ggatgagaac gtgcccccag
     2461 gtcgtgagca gagtggaaag ccacacagga aacacagcga ccgcaaggag aatggcggcg
     2521 gcaagccaaa ggtctaccag cctaaagaag accacctagc cagtctcaag gtccatctca
     2581 ccagcgtggt ggctgccaag cccagcgaaa aggctgatat aagattagat gaggcggagg
     2641 acccagcagc cgcgccaatc ctgtgggtta gcaaatgggt ggactactca gacaagtacg
     2701 gcctgggata ccagctctgt gacggcagcg tcggtgtcct cttcaacgac tcaacaagac
     2761 ttctccttca cgcaaatgca gacacgttgg agtacataga gagagacggg aatgaaaaat
     2821 actgccgtct aggatcttac gactcgacgc tccacaagaa agtcactctc ctcaaatact
     2881 tcaggaacta catgagtgaa cacctactca agatcaactt ctttgaggac cacaccaagc
     2941 ttatagtgtg tcccatgatg ggtgccgcca cctacatcga cgccaagagg aacttccgca
     3001 ccttccgact gaacctgatc gagaagcacg gctgcacccc ggacctctac gaccggatca
     3061 agtacgccaa caacatggtc aagaacatgc tggacaagaa gaccacgacg acaacggctg
     3121 cagcggtccc agcgcactag actagtgact gactaggatc tggttaccac taaaccagcc
     3181 tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg
     3241 ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt ttcttcacat
     3301 tctaaaaaaa aaaaaaaaaa aaaaaccccc cccccccccc ccccccctgc aggtcgactc
     3361 tagaggatcc ccgggcgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     3421 ttgttatccg ctcacaattc cacacaacat acgag
//
