LOCUS       sequence-362068-        2733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to see protein dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Poon J, Fries A, Wessel GM, Yajima M
  TITLE     Evolutionary modification of AGS protein contributes to formation of
            micromeres in sea urchins.
  JOURNAL   Nat Commun. 2019 Aug 22;10(1):3779. doi: 10.1038/s41467-019-11560-8.
  PUBMED    31439829
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-22"; volume: "10(1):3779. doi"; pages: " 
            10.1038/s41467-019-11560-8"
COMMENT     Sequence Label: membrane-HsPLK1-dead
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..774
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(212..230)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     480..499
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             820..840
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             853..897
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 atgctgtgct gtatgagaag aaccaaacag gttgaaaaaa atgatgacga ccaaaagatt
       61 ggcggcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      121 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      181 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      241 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      301 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      421 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      481 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      541 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      601 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      661 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      721 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga
      781 ggggatcggt ggagctccac cggtggcggc cgctctagag agaatcttta ttttcagggc
      841 gccgccaaat tcaaagaaac cgctgctgct aaattcgaac gccagcacat ggacagcgga
      901 ggtggaggta ctagtctcga gatgagtgct gcagtgactg cagggaagct ggcacgggca
      961 ccggccgacc ctgggaaagc cggggtcccc ggagttgcag ctcccggagc tccggcggcg
     1021 gctccaccgg cgaaagagat cccggaggtc ctagtggacc cacgcagccg gcggcgctat
     1081 gtgcggggcc gctttttggg caagggcggc tttgccaagt gcttcgagat ctcggacgcg
     1141 gacaccaagg aggtgttcgc gggcaggatt gtgcctaagt ctctgctgct caagccgcac
     1201 cagagggaga agatgtccat ggaaatatcc attcaccgca gcctcgccca ccagcacgtc
     1261 gtaggattcc acggcttttt cgaggacaac gacttcgtgt tcgtggtgtt ggagctctgc
     1321 cgccggaggt ctctcctgga gctgcacaag aggaggaaag ccctgactga gcctgaggcc
     1381 cgatactacc tacggcaaat tgtgcttggc tgccagtacc tgcaccgaaa ccgagttatt
     1441 catcgagacc tcaagctggg caaccttttc ctgaatgaag atctggaggt gaaaataggg
     1501 gattttggac tggcaaccaa agtcgaatat gacggggaga ggaagaagac cctgtgtggg
     1561 actcctaatt acatagctcc cgaggtgctg agcaagaaag ggcacagttt cgaggtggat
     1621 gtgtggtcca ttgggtgtat catgtatacc ttgttagtgg gcaaaccacc ttttgagact
     1681 tcttgcctaa aagagaccta cctccggatc aagaagaatg aatacagtat tcccaagcac
     1741 atcaaccccg tggccgcctc cctcatccag aagatgcttc agacagatcc cactgcccgc
     1801 ccaaccatta acgagctgct taatgacgag ttctttactt ctggctatat ccctgcccgt
     1861 ctccccatca cctgcctgac cattccacca aggttttcga ttgctcccag cagcctggac
     1921 cccagcaacc ggaagcccct cacagtcctc aataaaggct tggagaaccc cctgcctgag
     1981 cgtccccggg aaaaagaaga accagtggtt cgagagacag gtgaggtggt cgactgccac
     2041 ctcagtgaca tgctgcagca gctgcacagt gtcaatgcct ccaagccctc ggagcgtggg
     2101 ctggtcaggc aagaggaggc tgaggatcct gcctgcatcc ccatcttctg ggtcagcaag
     2161 tgggtggact attcggacaa gtacggcctt gggtatcagc tctgtgataa cagcgtgggg
     2221 gtgctcttca atgactcaac acgcctcatc ctctacaatg atggtgacag cctgcagtac
     2281 atagagcgtg acggcactga gtcctacctc accgtgagtt cccatcccaa ctccttgatg
     2341 aagaagatca ccctccttaa atatttccgc aattacatga gcgagcactt gctgaaggca
     2401 ggtgccaaca tcacgccgcg cgaaggtgat gagctcgccc ggctgcccta cctacggacc
     2461 tggttccgca cccgcagcgc catcatcctg cacctcagca acggcagcgt gcagatcaac
     2521 ttcttccagg atcacaccaa gctcatcttg tgcccactga tggcagccgt gacctacatc
     2581 gacgagaagc gggacttccg cacataccgc ctgagtctcc tggaggagta cggctgctgc
     2641 aaggagctgg ccagccggct ccgctacgcc cgcactatgg tggacaagct gctgagctca
     2701 cgctcggcca gcaaccgtct caaggcctcc taa
//
