LOCUS       sequence-362073-        3665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to see protein dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Poon J, Fries A, Wessel GM, Yajima M
  TITLE     Evolutionary modification of AGS protein contributes to formation of
            micromeres in sea urchins.
  JOURNAL   Nat Commun. 2019 Aug 22;10(1):3779. doi: 10.1038/s41467-019-11560-8.
  PUBMED    31439829
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-22"; volume: "10(1):3779. doi"; pages: " 
            10.1038/s41467-019-11560-8"
COMMENT     Sequence Label: SpPLK1-GFP
FEATURES             Location/Qualifiers
     source          1..3665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     194..213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     427..446
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     736..753
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           766..808
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(876..897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1137..1156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1484..1505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     3'UTR           3383..3508
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3607..3623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3607..3623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3620..3642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3631..3647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attaacctat aaaaaatagg cgtatcacga ggcccttttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acntntgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcttgt tctttttgca
      781 gaagctcaga ataaacgctc aactttggca gatctatgcc atcggggccc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct atacaagtcc ggaggggatc
     1561 ggtggagctc caccggtggc ggccgcatgg cttcaagaca taaggaagat cttagggtga
     1621 aggaagttcc tgatgttgtt actgatccat cgacgggaaa aacatacacg aaaggaagat
     1681 ttttgggcaa gggtggtttt gcaaagtgtt atgagctgac ggacgatgcc accaaacaga
     1741 tctttgctgg aaaggtcgtc tccaaggccc tacttgtcaa acctcaccaa aaagacaaga
     1801 tgacaatgga gattcacatt cataaaagcc tgcatcacag gcacgtcgtt ggtttccata
     1861 gcttctttga agacaaggaa aatgtctacg tcctactgga gctgtgcagg agaaggtcac
     1921 taatggagct gcacaagaga cgaaaggcca tcacagagcc cgagactcgc tacttcatgc
     1981 gtcagtgcat cctggcctgt cagtatctct ccaagaccaa ggtcattcac cgtgacctca
     2041 agctgggtaa cctcttcatc gacgacaaca tggagctcaa ggtcggggac tttggtctcg
     2101 ccaccaaggt cgacttcagc ggggaacgca agaagacact gtgtggaact cccaactaca
     2161 ttgccccgga agtgctttcc aagaagggtc acagctacga ggttgacctt tggtcactag
     2221 gatgcattat gtacaccctc ttggtgggga aacctccttt tgagactcag agtctgaaag
     2281 acacctacca gcgaatcaag agaaatgaat acagagtacc gtcccatgtc agtacaccgg
     2341 ctcgcaatct cattgttaaa ttactcaaaa acgaccctac gcagcgacct cacattgaca
     2401 tcctccttca agacgagttc ttcaccacag ggtacctgcc ccctcagctc cccaccacct
     2461 gtctcaccat ggctcctagg ttccaagtgc ctgtatcgtc aggcagaaga ccactcctag
     2521 aggtcaacgg ccaggatgag aatgtgcccc caggtcgtga gcagagtggg aagccgcaca
     2581 ggaaacacag cgaccgcaag gagaacggcg gcggcaagcc aaaggtctac cagcctaaag
     2641 aagaccacct agccagtctc aaggtccatc tgaccagcgt ggtggctgcc aagcccagcg
     2701 agaaggccga tataagatta gacgaggccg aggacccagc agctgcgcca atcctgtggg
     2761 ttagcaaatg ggtggactac tcggacaagt acggtctggg ataccagctc tgtgacggca
     2821 gcgtaggcgt actctttaac gactcaacaa gacttctcct tcacgcaaat gcagacacgt
     2881 tggagtacat cgagagagac gggaatgaga agtactgccg tctaggatct tacgactcaa
     2941 cgctccacaa gaaagtcacc ctcctcaaat acttcaggaa ctacatgagt gaacaccttc
     3001 tcaaggctgg tgctgccatg acgccccgtg aatcagacag catggccagg ctaccgttct
     3061 tacagagctg gttcaggacc aagagtgcca ttgtccttca tctcagcaat ggaaccgtcc
     3121 agatcaactt ctttgaggac cacaccaagc tgatagtgtg tcccatgatg ggcgccgcca
     3181 cctacatcga cgccaagagg aacttccgca ccttccgcct gaacctgatc gagaagcacg
     3241 gctgcacccc ggacctctac gaccggatca agtatgccaa caacatggtc aagaacatgc
     3301 tggacaagaa gaccacgacg acaacggccg cagcggtccc agcgcactag actagtgact
     3361 gactaggatc tggttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct
     3421 acataatacc aacttacact ttacaaaatg ttgtccccca aaatgtagcc attcgtatct
     3481 gctcctaata aaaagaaagt ttcttcacat tctaaaaaaa aaaaaaaaaa aaaaaccccc
     3541 cccccccccc ccccccctgc aggtcgactc tagaggatcc ccgggcgagc tcgaattcgt
     3601 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3661 acgag
//
