LOCUS       sequence-362076-        4079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to see protein dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Fernandez-Nicolas A, Xu D, Yajima M
  TITLE     A tumor suppressor Retinoblastoma1 is essential for embryonic 
            development in the sea urchin.
  JOURNAL   Dev Dyn. 2019 Dec;248(12):1273-1285. doi: 10.1002/dvdy.113. Epub 
            2019 Oct 1.
  PUBMED    31515896
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.113"; 
            journalName: "Dev Dyn"; date: "2019-12"; volume: "248"; issue: "12";
            pages: "1273-1285"
COMMENT     Sequence Label: SpVasa-E441Q-GFP
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     194..213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     427..446
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     736..753
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           766..808
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             join(3046..3048,3049..3051,3052..3765)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSXEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3091..3112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3352..3371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3699..3720
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     3'UTR           3797..3922
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(4021..4037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4021..4037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4034..4056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4045..4061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attaacctat aaaaaatagg cgtatcacga ggcccttttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acntntgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcttgt tctttttgca
      781 gaagctcaga ataaacgctc aactttggca gatctgatca tgccatggtt cgggcccatg
      841 tcagaagact gggggacctc ttcggtgtcg ggtggatcca gctttggaaa gagtagtttt
      901 ggtggatcaa gtggtggggt caaagacgat ggatttggcg ttggagtcgg tcgaggtcga
      961 ggaaggggtc gcgggtttag cccctttgct tcaaacggtg gtgtcggtgg tatgaccaat
     1021 gggactacca atggaggagg agagagtagc ggtgggtggg atgagagccc atctaagagt
     1081 ggctcaagta gcagtccatg gggcggtgat ggtgataatg ggttcggcaa gagtagcttt
     1141 ggtagcaaat cgttcggtgg aggaggagga ggtggtggtg gtgggggccg aggtgctgaa
     1201 tcaggaggag gacgaggagg aggaggattc ggcggcagac gaggaggacg tggaggtgga
     1261 gatagatcct gttataactg cggtgaaaca ggtcacatgt caagagaatg tcccacaaag
     1321 gactcttcag gaggaggacg tggtggtggt ggtggcgata gaagttgcta caattgcggt
     1381 gagaccggac acatgtcacg agaatgtccc acaaaagact cttcgggtgg aggaggtgga
     1441 ggtggtggaa agtgctttag atgtcaagaa gaaggccact ttgccaagga atgccccaat
     1501 ccagatacaa gaaatgaagg cgaaggtggt gaagccaagc cgccagcatc cacctacgtt
     1561 cctcccgacc catccgagga agaatcacag atttacataa gcactgaaca aggaatcaac
     1621 ttcaacaagt atgatgatat acccgtagaa gtcagcggtc gagacaggcc aaaaaatata
     1681 cgagcttttg aggaggccga gctagatgag acggtattgt ccaatgtgcg caaggctcac
     1741 tacgctaagc caacacctgt gcagaagtat ggtatgccca tcatttcttg tggacgtgat
     1801 ctgatggcct gtgcccaaac aggatcagga aagacggcgg cattcctcct acccatcatc
     1861 accaacatga tcacccacgg aggatgcatc agcaccttca acgtgatcca ggaaccactg
     1921 gccctcatcg tctcgccaac ccgcgagctg gccatccaga tctacaacga ggcacgcaag
     1981 ttctgccgcg gcaccaccat acgacctgtg gtggtatacg gaggcaccag cgtcagtcat
     2041 cagatgagag agtgtcagaa gggatgctcc atcctggtag ccaccccagg acgtatgcat
     2101 gacttcgtaa ccagaggata tatcggcctg ggcaagctga agttcctgat ccttgaccag
     2161 gccgaccgta tgttggacat gggtttcggt cccgacatcc agagactgat cgagcaccct
     2221 cacatgcctg ccaagggtga tcgtcaaacc ctgatgttca gtgcgacctt ccccaatgag
     2281 gtccaggaga aggcagggga gtacctcaac gactacctct tcctgacggt agggagggta
     2341 ggaggagctg cctcagacat cgagcagagg gtgttctcgg tcggtcaatt cgacaagcgg
     2401 gataagctga tggagatcct gggggatcag aaagatgatg accgtactct ggtgtttgtt
     2461 tcgaccaaga ggaatgctga tttcttggcc tctcttctca gtcaatctga attccccacc
     2521 accagcatcc atggtgaccg tcagcagcag gagcgtgagg aagccctgcg tgacttcaag
     2581 accggcaagg caccaatcct tgttgccacg tccgtcgccg ccagaggtct ggacatccca
     2641 ggggtcaagc acgtggtcaa ctacgacctc ccaagcgaca tcgatgagta cgtgcatcgc
     2701 atcgggcgta ccggacgtgt cggaaacctt ggacgatcaa ctagcttcta tgatgcagaa
     2761 aaggatgcta acattgcgag agctctcatt aagatcttgg ctgatgcgca gcaggaagta
     2821 ccggagttct tagaggaggc tgctgattca gccattggaa cctaccatgg caacgctgga
     2881 gggagctttg gaggcaggga caccaggaag ttcggtggtc gaggaggagg aaggggagga
     2941 ggaagtggcg gcggtggtgg agacgagtgg ggatctggag gaggaggcgg cggcggtggc
     3001 ggaggaggag ctgctgatga tgaatcatgg gatctcgagg atatcatggt gagcaagggc
     3061 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3121 cacaagttca gcgtgtccgn cgagggcgag ggcgatgcca cntacggcaa gctgaccctg
     3181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3241 acntacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3481 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3541 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3661 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3721 accgccgccg ggatcactct cggcatggac gagctgtaca agtaactagt gactgactag
     3781 gatctggtta ccactaaacc agcctcaaga acacccgaat ggagtctcta agctacataa
     3841 taccaactta cactttacaa aatgttgtcc cccaaaatgt agccattcgt atctgctcct
     3901 aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa aaaaaaaaac cccccccccc
     3961 cccccccccc ctgcaggtcg actctagagg atccccgggc gagctcgaat tcgtaatcat
     4021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgag
//
