LOCUS       sequence-362085-        4379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to see protein dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Wavreil FDM, Poon J, Wessel GM, Yajima M
  TITLE     Light-induced, spatiotemporal control of protein in the developing 
            embryo of the sea urchin.
  JOURNAL   Dev Biol. 2021 Oct;478:13-24. doi: 10.1016/j.ydbio.2021.06.006. Epub
            2021 Jun 18.
  PUBMED    34147471
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2021-10"; volume: "478:13-24. doi"; pages: " 
            10.1016/j.ydbio.2021.06.006. Epub 2021 Jun 18"
COMMENT     Sequence Label: GFP-SpBeta-catenin
FEATURES             Location/Qualifiers
     source          1..4379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     194..213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     427..446
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     736..753
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           766..808
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(876..897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1484..1505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     3'UTR           4097..4222
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(4321..4337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4321..4337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4334..4356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4345..4361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attaacctat aaaaaatagg cgtatcacga ggcccttttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acntntgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcttgt tctttttgca
      781 gaagctcaga ataaacgctc aactttggca gatctatgcc atcggggccc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct atacaagtcc ggaggggatc
     1561 ggtggagctc caccggtggc ggccgcatgg agcaggcaag gtactctaat caacagcagc
     1621 ctgtaaaccc acaacagaaa ggctatggcg gtccagggct tcaggacctc ccaaagcaag
     1681 atcctatgca gaataccatg gaatggcaac atcatggcta tggtatggat tcaggattcc
     1741 agagcaatgc cacctcacat gccccctctg tgagcagcaa gtcacgtgta gaggatggtg
     1801 gtggtggtgg cggtgaggag gccggtgctc agggcatgtt tgactgggat gctggctatg
     1861 gtcaggctta cacacaggaa caggttgacg agatgaacca gcagctgacc cagacacgct
     1921 ctcagcgtgt gagggcagcc atgttcccag agacattgga agagggagtt cagatcccat
     1981 ccacccagtt tgaccccgcc cacccgacgg ctgtacagcg cctttcagaa ccctcccaga
     2041 tgctcaagca tgccgtcgtc aacctcatca actaccaaga cgatgctgac ttggctacca
     2101 gggctatccc agagctcacc aagctcctca atgatgatga cttggttgtg gtgaaccagg
     2161 ctgctatgat ggtccaccag ctctccaaga aggaggccag ccgacatgcc atcatgaact
     2221 cccctcagat ggtggcagcc cttgtcagag ctatgtccaa cagcaacgat gctgagacta
     2281 ccagatgtgc tgccggcacc ctgcacaacc tctcccacca tagggcaggt ctgctccaga
     2341 tcttcaagag tggcggcatc ccagctctca tcaagctgct cagctcccca gtagagtctg
     2401 tacttttcta tgccatcaca accctccaca acctcctgct tcaccaagaa ggctccaaga
     2461 tggccgttcg tcttgcagga ggcctgcaga agatggtcgc tctgctctct cgcaacaatc
     2521 ctaaattctt ggccatcaca actgactgcc ttcagatcct ggcctacggg aaccaggaaa
     2581 gcaagttgat catcctagcc agcggtgggc cagcagctct ggtgcacatc atgcgtacat
     2641 atgagtatga gaagcttcta tggaccacca gcagggttct caaggtgctc tctgtctgtc
     2701 ataacaacaa accaggcatc gttgaagcag gaggaatgtc tgcccttggt ctccaccttg
     2761 gacatcaaag caatcgcttg gtccagaact gtctctggac cctcaggaat ctctcagact
     2821 gtcatcgtgg aactgatgac attgagccac tccttcagat gctggtacag ctcctagcct
     2881 ccaatgacat caacgtggtg acctgtgcct gcggcatcct gtctaacttg acctgtaaca
     2941 acacccgtaa caagatgatt gtcagtcaga tggctggagt tgaagccttg gtacagacac
     3001 tcatgaaggc tggagacaga gaggaaatca cagagccagc ggtatgtgcc cttcgtcatg
     3061 tgactagtcg ccacccagga gctgagatgt gtcagaatac agtgcgtctg aactatggta
     3121 tcccagtcat tgtgaagctc ctccatcctc cctctagatg gcctcttatc aaggccactg
     3181 ttggtctgat tcggaacctg gctctctgct ctgctaacca tgctccactc agagagcaag
     3241 gagctctaca cagactggta caacttctca tgagagccca tcaagacaca cagagacgct
     3301 catcgatggg tagcaccggc agccagggag gtaactatgc tgatggtgta cgtatggagg
     3361 acattgtgga aggtactaca ggagctcttc atatcctagc tagagactct cacaatcgag
     3421 cactcatcca aggactcaac tgcatccctc tctttgtaca gttgctgtat tccaacattg
     3481 agaacatcca gcgtgttgca gccggagtgt tgtctgagct gtccctagag aagcaaggtg
     3541 ctgagatgat tgaacaggaa ggtgctactg ctccccttac tgagctcctc cactccagga
     3601 acgagggagt tgcaacctat gcagcagcag tactgtaccg tatgtcagat gacaaacccc
     3661 aggactacaa gaagaggatc tcagttgagt taggcaactc tctcttcaga ggtgattcag
     3721 tgccatgggg tgatccactt gatatgccgt ctgacaatca gatccttcca ccaagctcta
     3781 tgggaggtca tccttcagac tcgagctatc cccaacatgg cagcgtacac agtcttcaca
     3841 gtaaccatgg agagtacagg caacctccac ctaccatgca aggctaccat gatggtactg
     3901 gacccattga gcctatgatg caggacctgg atctgggtgg aggaggagac tttggcatgg
     3961 atcctggtct ccctgacatg ggaccaccaa caaacgatct gagccttgac agtatcccac
     4021 cggcagacaa caccggcctg gcgttctttg ataccgatct gtaaactagt gactgactag
     4081 gatctggtta ccactaaacc agcctcaaga acacccgaat ggagtctcta agctacataa
     4141 taccaactta cactttacaa aatgttgtcc cccaaaatgt agccattcgt atctgctcct
     4201 aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa aaaaaaaaac cccccccccc
     4261 cccccccccc ctgcaggtcg actctagagg atccccgggc gagctcgaat tcgtaatcat
     4321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgag
//
