LOCUS       sequence-362086-       15643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9
            integration at ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15643)
  AUTHORS   Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, 
            Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito
            RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, 
            Spencer SL, Martin BL, Matus DQ
  TITLE     Visualizing the metazoan proliferation-quiescence decision in vivo.
  JOURNAL   Elife. 2020 Dec 22;9. pii: 63265. doi: 10.7554/eLife.63265.
  PUBMED    33350383
REFERENCE   2  (bases 1 to 15643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-22"; volume: "9. pii: 63265. doi"; pages: " 
            10.7554/eLife.63265"
COMMENT     Sequence Label: pAWK61
FEATURES             Location/Qualifiers
     source          1..15643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1507..1540)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1540..1551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2973..3296)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5214..5238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5241..6266
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5241..6266
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6267..6291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6644..6677)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7046..7063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7047..7063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9155..9211
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11339..11419
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      11428..11751
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(11784..11800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11784..11800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(13273..13293)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(13430..14018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13510..13529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14189..14980,14981..15049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14812..14831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15050..15154)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15222..15240
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15278..15300)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    15623..15643
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcctact gcgcatggaa
      181 ataggcggta gtcaggcagg tagatagcct cataaaattc aatacttcct tttcagaaag
      241 aatgctggaa tgtattcaaa gaggagtcac tcttcccatt gctcagagaa tatgaagagt
      301 tgctcggcga gcaaaagttc atgattggag atcgatattc atgggctgac atcgctgtcg
      361 tcgagtttct aactcggtgt cagcagtgct acgatagctt ctatctggct catttcgaga
      421 ggctccgaac tttttgccag acttttgagt ctcttccgca cattcgtccc tacattcagg
      481 gacgtgttga ttcatttatc taatttttta aaatggatat ttttcgtttt ttatttgttt
      541 gttttgctga taattcaata aagttacgca acgaatttta gacatttatg aaaaccgaaa
      601 atcgacaaaa atattttaag aaaaatagca gcaaaattca atattttatc aaattttgtt
      661 tatggtaatg agaaagaaaa catacaatat ttttctaaga agaagaaaaa tgaccagaat
      721 tttaagattt ttctcgaata tacgaaaaat tgataaacta aaattttcga tttttccatt
      781 tattttgaaa aaaaaactct tgacggtttt caccatgcga agaggtgaga aatattctga
      841 aagttatgca ttttgaaaaa attgcaaaaa aatacgttaa aattttttga tctattgttt
      901 ttttttgaaa accggaattt aaaatggaat tttttttcaa aaattaaaat taaaatttaa
      961 atatttctag tgtttggata gtttttcaaa agctagattt aacccgcgag atgtcgaccg
     1021 ctagtgtagc ttacagatgg ctgaagactt cgcaaaaatc ttatcttaga aacccgcgag
     1081 gtgtcggccg ctaaaacttt gtaaacctgc caacaaaatg cttcgcattg tctgaacaga
     1141 tgaaaccttg aaacaaaaca tatacaattg aaatcccgta aagtgtcggg cgcaagaact
     1201 gcgattagtt gtataattgg actagctcaa atttgaccaa aatctcattt ttagacctct
     1261 aagatgtcgg ccactataac tttattcaac ttgaaactgc aaaaaaactt atcaactgta
     1321 tagattcaag tattatgtca agtattggtt ttagagctac agtacactct gataacaaaa
     1381 tctgcagaaa tacctccctg tcaattccca aaatacctcc cctcatctca attatcccga
     1441 aaatcggtta gtcattgcca tcagaaaatc gaattaggta ccatgatggt agcaaactca
     1501 cttcgtataa cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct
     1561 tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa
     1621 ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta
     1681 ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg
     1741 tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa
     1801 aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta
     1861 catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa
     1921 aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga
     1981 ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga
     2041 atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca
     2101 atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg
     2161 ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc
     2221 tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg
     2281 agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc
     2341 atccggacca aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg
     2401 tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac
     2461 ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact
     2521 tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg
     2581 agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa
     2641 gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca
     2701 aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac
     2761 cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg
     2821 agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa
     2881 gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg
     2941 tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt
     3001 ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt
     3061 tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac
     3121 atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac
     3181 tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt
     3241 gacaaaaaga aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat
     3301 cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa
     3361 tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg
     3421 aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag
     3481 ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga
     3541 agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt
     3601 caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa
     3661 cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct
     3721 caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg
     3781 tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga
     3841 cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg
     3901 cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga
     3961 agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc
     4021 tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac
     4081 gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat
     4141 ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt
     4201 ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt
     4261 taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct
     4321 cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg
     4381 tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt
     4441 cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt
     4501 atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg
     4561 aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt
     4621 gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc
     4681 gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa
     4741 catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta
     4801 tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac
     4861 acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc
     4921 aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt
     4981 cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa
     5041 aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc
     5101 agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta
     5161 tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt
     5221 tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5281 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5341 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5401 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5461 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5521 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5581 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5641 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5701 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5761 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5821 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5881 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5941 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6001 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6061 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6121 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6181 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6241 gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac
     6301 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6361 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6421 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6481 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6541 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6601 accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat
     6661 accttatacg aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata
     6721 tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg
     6781 tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa
     6841 ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga
     6901 gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac
     6961 aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat
     7021 tcaacgacgt tggcgtcgat catcctgtaa aacgacggcc agtgcggccg cttcaatcgg
     7081 tttttccttg cttgcgccca attctgatgg ttcttccatt tctaaatttt agtttaataa
     7141 aaaaatttta aaataatacg agttttgaga ctaactttca ataaaaacca aaaaaaattg
     7201 cttcaaaaaa gagttcgcgc cgatttacga taaacacagc gcgctacgac actccgaaaa
     7261 ccgcgccgca ccattacatt ctccgcccca ccgcacggtt tgcggaaggc gatgaaattg
     7321 ctgggcctaa aattttaacc ctttttttcc cgtttttcaa ctgttttccc gattttttcg
     7381 atattttttt caggttttta gttagaaaag tatataaaaa ttgcattatt tgtagttttc
     7441 gctcgtaatt tgtgatagaa aacggccaat ttgcctagat ttgtgattct accaagtgga
     7501 atttttggga aaatatcaat attccacgat ttttattcaa atcataacgt ttcgaaatta
     7561 tcagcttaaa taaaactaaa ataaataata aaatactaaa aaccctgaat ttctcggttc
     7621 tcggttcagc ttgagttcgt tctatacacg ttatcgattt ttgtcatttc gtttaatcat
     7681 gggttttgat tcattttaaa tatgaaattt gatttattcc tcgtttcgaa gttggtttgt
     7741 cttgtccggc cggtaaacaa atgttattaa aaaaataata aatttcgaaa tttttcgtaa
     7801 tgttccgcaa tcgtgttttt ccgtttatct cgtcttccct tctttcaaat atactattta
     7861 ttttctcaat tcacatattt tcagccagcc ccgctcaaca agtggaataa aaatgaccaa
     7921 cgacgtcacc tggtccgagg cctcctcccc agacgagcgt accctcacct tcgccgagcg
     7981 ttggcaactc tcctccccag acggagtcga caccgacgac gacctcccaa agtcccgtgc
     8041 ctccaagcgt acctgcggag tcaacgacga cgagtcccca tccaaggtaa gtttaaacat
     8101 atatatacta actaaccctg attatttaaa ttttcagatc ttcatggtcg gagagtcccc
     8161 acaagtctcc tcccgtctcc aaaacctccg tctcaacaac ctcatcccac gtcaactctt
     8221 caagccaacc gacaaccaag agaccggagc atcgggagcc tcaggagcat cgatgagtaa
     8281 aggagaagaa ttgttcactg gagttgtccc aatcctcgtc gagctcgacg gagacgtcaa
     8341 cggacacaag ttctccgtct ccggagaggg agagggagac gccacctacg gaaagctcac
     8401 cctcaagttc atctgcacca ccggaaagct cccagtccca tggccaaccc tcgtcaccac
     8461 cttctgctac ggagtccaat gcttctcccg ttacccagac cacatgaagc gtcacgactt
     8521 cttcaagtcc gccatgccag agggatacgt ccaagagcgt accatcttct tcaaggtaag
     8581 tttaaacata tatatactaa ctactgatta tttaaatttt caggacgacg gaaactacaa
     8641 gacccgtgcc gaggtcaagt tcgagggaga caccctcgtc aaccgtatcg agctcaaggt
     8701 aagtttaaac agttcggtac taactaacca tacatattta aattttcagg gaatcgactt
     8761 caaggaggac ggaaacatcc tcggacacaa gctcgagtac aactacaact cccacaacgt
     8821 ctacatcatg gccgacaagc aaaagaacgg aatcaaggtc aacttcaagg taagtttaaa
     8881 catgatttta ctaactaact aatctgattt aaattttcag atccgtcaca acatcgagga
     8941 cggatccgtc caactcgccg accactacca acaaaacacc ccaatcggag acggaccagt
     9001 cctcctccca gacaaccact acctctccac ccaatccgcc ctctccaagg acccaaacga
     9061 gaagcgtgac cacatggtcc tcctcgagtt cgtcaccgcc gccggaatca cccacggaat
     9121 ggacgagctc tacaagtcag gagctggatc cggagccacc aacttctccc tcctcaagca
     9181 agccggagac gtcgaggaga acccaggacc aatgccacca aagccatctg ccaagggagc
     9241 caagaaggcc gccaagaccg tcgttgccaa gccaaaggac ggaaagaaga gacgtcatgc
     9301 ccgcaaggaa tcgtactccg tctacatcta ccgtgttctc aagcaagttc acccagacac
     9361 cggagtctcc tccaaggcca tgtctatcat gaactccttc gtcaacgatg tattcgaacg
     9421 catcgcttcg gaagcttccc gtcttgctca ttacaacaaa cgctcaacga tctcatcccg
     9481 cgaaattcaa accgctgtcc gtttgattct cccaggagaa cttgccaagc acgccgtgtc
     9541 tgagggaacc aaggccgtca ccaagtacac ttccagcaag ctcgagatgg tctccgagct
     9601 cattaaagaa aacatgcata tgaagctcta catggaggga accgtcaaca accaccactt
     9661 caagtgcacc tccgagggag agggaaagcc atacgaggga acccaaacca tgcgtatcaa
     9721 ggccgtcgag ggaggaccac tcccattcgc cttcgacatc ctcgccacct ccttcatgta
     9781 cggatccaag gtaagtttaa acatatatat actaactaac cctgattatt taaattttca
     9841 gaccttcatc aaccacaccc aaggaatccc agacttcttc aagcaatcct tcccagaggg
     9901 attcacctgg gagcgtgtca ccacctacga ggacggagga gtcctcaccg ccacccaaga
     9961 cacctccctc caagacggat gcctcatcta caacgtcaag gtaagtttaa acagttcggt
    10021 actaactaac catacatatt taaattttca gatccgtgga gtcaacttcc catccaacgg
    10081 accagtcatg caaaagaaga ccctcggatg ggaggcctcc accgagaccc tctacccagc
    10141 cgacggagga cttgagggac gtgccgacat ggccctcaag ctcgtcggag gaggacacct
    10201 catctgcaac ctcaaggtaa gtttaaacat gattttacta actaactaat ctgatttaaa
    10261 ttttcagacc acctaccgtt ccaagaagcc agccaagaac ctcaagatgc caggagtcta
    10321 ctacgtcgac cgtcgtcttg agcgtatcaa ggaggccgac aaggagacct acgtcgagca
    10381 acacgaggtc gccgtcgccc gttactgcga cctcccatcc aagctcggac accgtggtgg
    10441 aggaggatct ggaggaggag gatccatggt ttccgagttg atcaaggaga atatgcacat
    10501 gaaactctac atggaaggaa ccgtcaacaa tcatcatttc aaatgtactt cagaaggaga
    10561 aggaaaacca tacgagggaa ctcagaccat gagaatcaag gctgttgagg gaggtaagtt
    10621 taaaaaatac gtgttcataa cttgatacga atataaattt tcaggaccac ttccattcgc
    10681 gttcgacatt cttgccactt cgttcatgta tggaagcaag actttcatta atcacacaca
    10741 agggatcccg gatttcttca agcaatcttt cccagaagga ttcacatggg agagagttac
    10801 cacttacgag gatggaggag ttcttaccgc cacccaagac acatccctcc aaggtaagtt
    10861 taaaagatta tagcaatgtt cgtctaaata taataaattt tcagatggat gtctgatcta
    10921 caacgtcaag atcagaggag tcaatttccc atcaaatgga ccagttatgc agaagaagac
    10981 tttgggctgg gaggcttcca ccgagaccct ttacccagca gatggaggtc tcgagggccg
    11041 cgctgatatg gctctcaaac tcgtcggagg aggacacttg atctgcaatt taaaaaccac
    11101 ctaccggtcg aaggtaagtt taaaatcaat ttttaatagc ttatataata atacaaattt
    11161 tcagaagcca gccaagaatc tcaaaatgcc aggagtttac tatgttgacc gtcgtcttga
    11221 gcgcattaaa gaggccgata aggaaacgta cgttgaacaa catgaagttg ccgttgctcg
    11281 atattgtgat ctcccatcca agctcggaca tcgtgccggc tcaggagcta gcggagccta
    11341 cccttacgac gtgccagatt acgcttaccc ctacgacgta ccagactacg cctacccata
    11401 cgacgtccca gactacgctt aagataaatg caaaatcctt tcaagcattc ccttcttctc
    11461 tatcactctt ctttcttttt gtcaaaaaat tctgtcgcta atttatttgc ttttttaatg
    11521 ttattatttt atgacttttt atagtcactg aaaagtttgc atctgagtga agtgaatgct
    11581 atcaaaatgt gattctgtct gatgtacttt cacaatctct cttcaattcc attttgaagt
    11641 gctttaaacc cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt
    11701 gagtgaccca acaaaaagag gaaactttat tgtgccgcca agaaaaaagt ctcacgtacg
    11761 gtgcgcgcga tgccctaggc atggtcatag ctgtttcctg cgcaagtcgg cgatttcttt
    11821 gaagttttgt caaaagaaga gacaaggact tgatagatac gctcatcaga gtgagattca
    11881 ttgcaaatag tgtgtaaatc gtggagagac gagagaaaag gtgtgagaag cttagaagat
    11941 aaagaatagg aagaagaaca tatctcattt cctattttct ctaatttttc gttcattttt
    12001 ctagacatta cccatttgat ggtttttcaa tatggttatc agggtactca ttttggtagt
    12061 tttcaaagca atttggagtt tttttttaaa ttgtcaacta caaaaacgca gcttaaaggt
    12121 ccaaatatca tagtataaaa attctttaaa aaattccaaa ttttatatga ttttttgaaa
    12181 attggaagaa tgttagtttt tgccattttt ttggaagttt ttctccggat tttatggtac
    12241 ttatatttca aatataaatt tttgtaggaa ttcgagtatg cgagagagct atagtttact
    12301 aaaaaaaagt ggcaaaaact cagtaattgc cacttttttc aaaaatgttt tgaaaaattt
    12361 tactatgata tttggccatt ttggcaccat atgtacgtta ctttttacga aacactctaa
    12421 tttttgaaat ttgagtttca tgtttaatct tttgtataaa atttttatgg tccttttgtg
    12481 acaggataaa aaattgcaaa aaaatagaaa actttgtttc gtgaaaacac ctagtttaaa
    12541 aattgtgggc cacttagcga ttctagtcaa aaatcagaca aatgtcagct gccgtaactt
    12601 tttccggatc agggttatca aattttaatt ttaattgtaa atgtagaaaa atgatcggca
    12661 acattttgct aattgaaaat attttgaaaa ttgctatagt acatattaat ctagtattaa
    12721 tatagtatta atgttataat atcagttatg tgtttgaata actcaagcgt aagtttccgt
    12781 aaaatatctt taaattttgg taatttttta cggtttggta taaatttttg cattttttga
    12841 ttttctacac tttttttcga tttcgcattt tccgaatttc tctgaaataa atcgaacaat
    12901 cggaaaattt tcgagggcac ccttagccat gcagctcagg aatttgactt tatgttccca
    12961 ttcaaatttt tttaaaatta aataaaatgc taattgtaag ataaattaca aaaattatta
    13021 aatctcatca tccttatgat gatagtgcag tgcatgaata tatttttcca aataggatat
    13081 gattcgtcga gtagcttcac gtaatccgtt ttcttcaatc accatttcga actgcaattt
    13141 tttttttgga tttttgtaac aatattgtca taaattgtga ttttgatatt ttgcgtagag
    13201 catcagaacc tttctgcttt gaattttaat gggattaaaa attataaaaa acttgcaccc
    13261 taattaaagc gccaacttta ttatacatag ttgataattc gatggtatca gctcactcaa
    13321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    13381 aaggccagca aaagaccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    13441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    13501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    13561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    13621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    13681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    13741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13861 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    13921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    14041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    14101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    14161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    14221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    14281 cgatacggga gggcttacca tctgacccca gtgctgcaat gataccgcga gacccacgct
    14341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    14401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    14461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    14521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    14581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    14641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    14701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    14761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    14821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    14881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    14941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    15001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    15061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    15121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    15181 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    15241 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    15301 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    15361 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgcac
    15421 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    15481 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    15541 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    15601 cgccagggat ccgccaagct atcaactttg tatagaaaag ttg
//
