LOCUS       sequence-362097-       10997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 
            1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be 
            integrated with pBac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Kandul NP, Liu J, Buchman A, Shriner IC, Corder RM, Warsinger-Pepe 
            N, Yang T, Yadav AK, Scott MJ, Marshall JM, Akbari OS
  TITLE     Precision Guided Sterile Males Suppress Populations of an Invasive 
            Crop Pest
  JOURNAL   GEN Biotechnology (2022) 1:4, 372-385
REFERENCE   2  (bases 1 to 10997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GEN 
            Biotechnology (2022) 1:4, 372-385"
COMMENT     Sequence Label: 1056H
FEATURES             Location/Qualifiers
     source          1..10997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1060..1479
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        1500..1575
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     repeat_region   2472..2506
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2687..2703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2687..2703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2700..2722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2711..2727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2735..2741,2742..2759,2760..2765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2780..2801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2918..2935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3089..3677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3169..3188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3848..4639,4640..4708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4471..4490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4709..4813)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4881..4899
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4937..4959)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5059..5078
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5272..5294
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5286..5303
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5287..5303
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   6007..6069
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        8065..8484
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8505..8580
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(9369..9371,9372..9374,9375..10088)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9414..9435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9675..9694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10022..10043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10207..10328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10244..10263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10298..10317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        10342..10761
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        10782..10857
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttttccgttg acgtacatac atctgacgtg tgtttattta gacataatag ttatgttttc
       61 acatcttttt aatgttcgct taatgcgtat gcatctcgag cctatctatg caaatctcag
      121 tgtgggatgt aagccaagtt gagtactttt tcttgaaatg attaaaattt cttttgcaat
      181 atgtctattt gcgggacaat ctataacaaa aagtttcgct ttgttttttt aaaacatcct
      241 cataagcatc tgaaacagct attgaagctc taaatctgtc aaatattgcc tgctgtttct
      301 cacgcttttt ctctccagaa ctccaagtct caaacaattc tgaattggcc aacctgaggt
      361 tgtgacctaa aaatgtatat tttagtgtta gtttttattg ttaatttgta cacgtaaagc
      421 ggcaattaaa agcactcaac acaaacaaat ccaacttgtg acgaccacaa acatatagta
      481 cgaaccgctc tctttcgatg ccatgacaac ttgtttgata taaacttaga ttggtgcttt
      541 atttttaata attttaaacg gactcttttt tccgcagagt agctttcgtg cacccgtgat
      601 accgaacagc tgttttaaca gcctatatat tttaacagat cgtgactcag ataaaatttc
      661 tgttacgtaa cattgaagtt tgattttaat attaacgact ttcaaaaaat ggatttttgc
      721 ccacaaaacg tgatcagcca gcctaaagtg cactcgctgc aaataggacc gcacgcgtaa
      781 tgtattttag tattttctac gagttaaatt aatttttgta tttgacgttt cgggatgttt
      841 ttaaaaaagg tagttgttgt tgcggcagta cccagtagcc ttccagcggc tcgaaactga
      901 acatttgatc tcatcctgaa aagaaatatt gctatgctca tttctaagaa aatgaccaac
      961 ctccatcttg tgtcctgctt ggcaagactt tgcacctgtt gcaccaaaag tacattattt
     1021 tcactacgct tgcttgaata gaattctcat cggcgcgcct ttttgctcac ctgtgattgc
     1081 tcctactcaa atacaaaaac atcaaatttt ctgtcaataa agcatattta tttatattta
     1141 ttttacagga aagaattcct tttaaagtgt attttaacct ataatgaaaa acgattaaaa
     1201 aaaatacata aaataattcg aaaatttttg aatagcccag gttgataaaa attcatttca
     1261 tacgttttat aacttatgcc cctaagtatt ttttgaccat agtgtttcaa ttctacatta
     1321 attttacaga gtagaatgaa acgccaccta ctcagccaag aggcgaaaag gttagctcgc
     1381 caagcagaga gggcgccagt gctcactact ttttataatt ctcaacttct ttttccagac
     1441 tcagttcgta tatatagacc tattttcaat ttaacgtcgt ggcggtaccg gctccggaag
     1501 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     1561 gcaccgagtc ggtgcttttt tgcctacctg gagcctgaga gttgttcaat aaaataaaaa
     1621 tgtttcgttt ttttgctttc gccagtattt attatttttc atcaatatgt attcaatttg
     1681 gtatgtattt agtaattgta atatatagac aatggttttc cgttgacgta catacatctg
     1741 acgtgtgttt atttagacat aatagttatg ttttcacatc tttttaatgt tcgcttaatg
     1801 cgtatgcatc ctaggccggc cgatctcgga tctgacaatg ttcagtgcag agactcggct
     1861 acgcctcgtg gactttgaag ttgaccaaca atgtttattc ttacctctaa tagtcctctg
     1921 tggcaaggtc aagattctgt tagaagccaa tgaagaacct ggttgttcaa taacattttg
     1981 ttcgtctaat atttcactac cgcttgacgt tggctgcact tcatgtacct catctataaa
     2041 cgcttcttct gtatcgctct ggacgtcatc ttcacttacg tgatctgata tttcactgtc
     2101 agaatcctca ccaacaagct cgtcatcgct ttgcagaaga gcagagagga tatgctcatc
     2161 gtctaaagaa ctacccattt tattatatat tagtcacgat atctataaca agaaaatata
     2221 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     2281 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     2341 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     2401 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     2461 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa aaaagatttg
     2521 cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca aaaaccacat
     2581 tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt cgcgcgagcg
     2641 tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
     2701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4801 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4861 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4921 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4981 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5041 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5101 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     5161 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     5221 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     5281 gacgttgtaa aacgacggcc agtgccaagc tttgtttaaa aatataacaa aattgtgatc
     5341 ccacaaaatg aagtggggca aaatcaaata attaatagtg tccgtaaact tgttggtctt
     5401 caactttttg aggaacacgt tggacggcaa atccgtgact ataacacaag ttgatttaat
     5461 aattttagcc aacacgtcgg gctgcgtgtt ttttgccgac gcgtctgtgt acacgttgat
     5521 taactggtcg attaaactgt tgaaataatt taatttttgg ttcttcttta aatctgtgat
     5581 gaaatttttt aaaataactt taaattcttc attggtaaaa aatgccacgt tttgcaactt
     5641 gtgagggtct aatatgaggt caaactcagt aggagtttta tccaaaaaag aaaacatgat
     5701 tacgtctgta cacgaacgcg tattaacgca gagtgcaaag tataagaggg ttaaaaaata
     5761 tattttacgc accatatacg catcgggttg atatcgttaa tatggatcaa tttgaacagt
     5821 tgattaacgt gtctctgctc aagtctttga tcaaaacgca aatcgacgaa aatgtgtcgg
     5881 acaatatcaa gtcgatgagc gaaaaactaa aaaggctaga atacgacaat ctcacagaca
     5941 gcgttgagat atacggtatt cacgacagca ggctgaataa taaaaaaatt agaaactatt
     6001 atttaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat
     6061 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
     6121 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
     6181 tttatttatt tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact
     6241 tttaaacatt ctctctttta caaaaataaa cttattttgt actttaaaaa cagtcatgtt
     6301 gtattataaa ataagtaatt agcttaactt atacataata gaaacaaatt atacttatta
     6361 gtcagtcaga aacaactttg gcacatatca atattatgct ctcgacaaat aacttttttg
     6421 cattttttgc acgatgcatt tgcctttcgc cttattttag aggggcagta agtacagtaa
     6481 gtacgttttt tcattactgg ctcttcagta ctgtcatctg atgtaccagg cacttcattt
     6541 ggcaaaatat tagagatatt atcgcgcaaa tatctcttca aagtaggagc ttctaaacgc
     6601 ttacgcataa acgatgacgt caggctcatg taaaggtttc tcataaattt tttgcgactt
     6661 tgaacctttt ctcccttgct actgacatta tggctgtata taataaaaga atttatgcag
     6721 gcaatgttta tcattccgta caataatgcc ataggccacc tattcgtctt cctactgcag
     6781 gtcatcacag aacacatttg gtctagcgtg tccactccgc ctttagtttg attataatac
     6841 ataaccattt gcggtttacc ggtactttcg ttgatagaag catcctcatc acaagatgat
     6901 aataagtata ccatcttagc tggcttcggt ttatatgaga cgagagtaag gggtccgtca
     6961 aaacaaaaca tcgatgttcc cactggcctg gagcgactgt ttttcagtac ttccggtatc
     7021 tcgcgtttgt ttgatcgcac ggttcccaca atggttaatt cgagctcgcc cggggtttaa
     7081 acacccgtac tttaagttat cctgatttgg aaaaaaattt taacatttga agtttttatt
     7141 gtttagaaaa ttcaatggag tcgccccaca tatgggacag ggttggcttt atctggtgcc
     7201 tgtcagtgtg tttaatactt ttccatctat tactgtcaag tataaactaa agtttatata
     7261 gaccgttttc ccattttcat aaacaaattt ggaagactgc aaaatcttat tttgcttctc
     7321 caagtttgta tgctccttta ttaagcattc ctttgtttcc tttatgaatt tcaactttag
     7381 agacctgcag aacctgatag attgtggaaa tcaattgttc catagtatat ttccgctaga
     7441 atcaatcatt cgtaaaggag tttctgctgt ggcgaatata ctgccatcga catcacactt
     7501 ctgtggacct gcaaatccct gtttgtagtt cgaatgacct gagcttccat cgtatccata
     7561 actaagtaga agttcggcga ttaaaaatgt tgtcccttga tctttcctta ttttctaaac
     7621 aacttttttt tgtaaagtta aaatccggcc agctgtgtgg ttaagcaaac ttagcaaagg
     7681 cacttctgct tttgtgtcat tcgttctttt tgtttgcaac ttcgtagtac gaggaaaaag
     7741 gttgtttttc gtttgatctc taattgagat attttgttgc ttggttaaat tttgatctta
     7801 aagaacagct agtgcgtcgt taaatagtat tttctccaat ttcgcgaggg gctgacttat
     7861 taaggtgtgc atgctcttgg ctttttcagg gcagatagca aacagtttta taatacatgc
     7921 cagatctgat ttactgccct tacgcgcagc cgcactagct gaatttatta ataatttaat
     7981 atttccagcc gtggatttga tcaaatttta agctgctctt ctctgagccc ttgctctcga
     8041 caacaaataa ggcaatgcgg ccgctttttg ctcacctgtg attgctccta ctcaaataca
     8101 aaaacatcaa attttctgtc aataaagcat atttatttat atttatttta caggaaagaa
     8161 ttccttttaa agtgtatttt aacctataat gaaaaacgat taaaaaaaat acataaaata
     8221 attcgaaaat ttttgaatag cccaggttga taaaaattca tttcatacgt tttataactt
     8281 atgcccctaa gtattttttg accatagtgt ttcaattcta cattaatttt acagagtaga
     8341 atgaaacgcc acctactcag ccaagaggcg aaaaggttag ctcgccaagc agagagggcg
     8401 ccagtgctca ctacttttta taattctcaa cttctttttc cagactcagt tcgtatatat
     8461 agacctattt tcaatttaac gtcggattgt caactacttg ccccgtttta gagctagaaa
     8521 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     8581 ttttttgcct acctggagcc tgagagttgt tcaataaaat aaaaatgttt cgtttttttg
     8641 ctttcgccag tatttattat ttttcatcaa tatgtattca atttggtatg tatttagtaa
     8701 ttgtaatata tagacaatgg ttttccgttg acgtacatac atctgacgtg tgtttattta
     8761 gacataatag ttatgttttc acatcttttt aatgttcgct taatgcgtat gcattaatta
     8821 acatactcgg tggcctcccc accaccaact tttttgcact gcaaaaaaac acgcttttgc
     8881 acgcgggccc atacatagta caaactctac gtttcgtaga ctattttaca taaatagtct
     8941 acaccgttgt atacgctcca aatacactac cacacattga acctttttgc agtgcaaaaa
     9001 agtacgtgtc ggcagtcacg taggccggcc ttatcgggtc gcgtcctgtc acgtacgaat
     9061 cacattatcg gaccggacga gtgttgtctt atcgtgacag gacgccagct tcctgtgttg
     9121 ctaaccgcag ccggacgcaa ctccttatcg gaacaggacg cgcctccata tcagccgcgc
     9181 gttatctcat gcgcgtgacc ggacacgagg cgcccgtccc gcttatcgcg cctataaata
     9241 cagcccgcaa cgatctggta aacacagttg aacagcatct gttacagcga cacaacatga
     9301 gccggtccaa caacgccaac gcgcccacgc catccaaccg ccgccgcaac ctgtctctgg
     9361 tggctagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     9421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     9481 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
     9541 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
     9601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     9661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     9721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     9781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     9841 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     9901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     9961 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
    10021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
    10081 acaagtaagc gctgactcta gatcataatc agccatacca catttgtaga ggttttactt
    10141 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10201 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10261 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10321 gtatcttaaa gcttccttaa gtttttgctc acctgtgatt gctcctactc aaatacaaaa
    10381 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
    10441 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
    10501 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
    10561 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
    10621 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
    10681 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
    10741 cctattttca atttaacgtc gggcggcagc ggcgggaatg ggttttagag ctagaaatag
    10801 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
    10861 tttgcctacc tggagcctga gagttgttca ataaaataaa aatgtttcgt ttttttgctt
    10921 tcgccagtat ttattatttt tcatcaatat gtattcaatt tggtatgtat ttagtaattg
    10981 taatatatag acaatgg
//
