LOCUS       sequence-362100-       10997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 
            1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be 
            integrated with pBac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Kandul NP, Liu J, Buchman A, Shriner IC, Corder RM, Warsinger-Pepe 
            N, Yang T, Yadav AK, Scott MJ, Marshall JM, Akbari OS
  TITLE     Precision Guided Sterile Males Suppress Populations of an Invasive 
            Crop Pest
  JOURNAL   GEN Biotechnology (2022) 1:4, 372-385
REFERENCE   2  (bases 1 to 10997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GEN 
            Biotechnology (2022) 1:4, 372-385"
COMMENT     Sequence Label: 1056J
FEATURES             Location/Qualifiers
     source          1..10997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5071..5490
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        5511..5586
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(6375..6377,6378..6380,6381..7094)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6420..6441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6681..6700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7028..7049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7213..7334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7250..7269)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7304..7323
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7348..7767
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        7788..7863
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        9063..9482
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        9503..9578
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     repeat_region   10475..10509
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10690..10706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10690..10706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10703..10725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10714..10730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10738..10744,10745..10762,10763..10768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10783..10804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10921..10938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtttaaacac ccgtacttta agttatcctg atttggaaaa aaattttaac atttgaagtt
     4141 tttattgttt agaaaattca atggagtcgc cccacatatg ggacagggtt ggctttatct
     4201 ggtgcctgtc agtgtgttta atacttttcc atctattact gtcaagtata aactaaagtt
     4261 tatatagacc gttttcccat tttcataaac aaatttggaa gactgcaaaa tcttattttg
     4321 cttctccaag tttgtatgct cctttattaa gcattccttt gtttccttta tgaatttcaa
     4381 ctttagagac ctgcagaacc tgatagattg tggaaatcaa ttgttccata gtatatttcc
     4441 gctagaatca atcattcgta aaggagtttc tgctgtggcg aatatactgc catcgacatc
     4501 acacttctgt ggacctgcaa atccctgttt gtagttcgaa tgacctgagc ttccatcgta
     4561 tccataacta agtagaagtt cggcgattaa aaatgttgtc ccttgatctt tccttatttt
     4621 ctaaacaact tttttttgta aagttaaaat ccggccagct gtgtggttaa gcaaacttag
     4681 caaaggcact tctgcttttg tgtcattcgt tctttttgtt tgcaacttcg tagtacgagg
     4741 aaaaaggttg tttttcgttt gatctctaat tgagatattt tgttgcttgg ttaaattttg
     4801 atcttaaaga acagctagtg cgtcgttaaa tagtattttc tccaatttcg cgaggggctg
     4861 acttattaag gtgtgcatgc tcttggcttt ttcagggcag atagcaaaca gttttataat
     4921 acatgccaga tctgatttac tgcccttacg cgcagccgca ctagctgaat ttattaataa
     4981 tttaatattt ccagccgtgg atttgatcaa attttaagct gctcttctct gagcccttgc
     5041 tctcgacaac aaataaggca atgcggccgc tttttgctca cctgtgattg ctcctactca
     5101 aatacaaaaa catcaaattt tctgtcaata aagcatattt atttatattt attttacagg
     5161 aaagaattcc ttttaaagtg tattttaacc tataatgaaa aacgattaaa aaaaatacat
     5221 aaaataattc gaaaattttt gaatagccca ggttgataaa aattcatttc atacgtttta
     5281 taacttatgc ccctaagtat tttttgacca tagtgtttca attctacatt aattttacag
     5341 agtagaatga aacgccacct actcagccaa gaggcgaaaa ggttagctcg ccaagcagag
     5401 agggcgccag tgctcactac tttttataat tctcaacttc tttttccaga ctcagttcgt
     5461 atatatagac ctattttcaa tttaacgtcg gattgtcaac tacttgcccc gttttagagc
     5521 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     5581 cggtgctttt ttgcctacct ggagcctgag agttgttcaa taaaataaaa atgtttcgtt
     5641 tttttgcttt cgccagtatt tattattttt catcaatatg tattcaattt ggtatgtatt
     5701 tagtaattgt aatatataga caatggtttt ccgttgacgt acatacatct gacgtgtgtt
     5761 tatttagaca taatagttat gttttcacat ctttttaatg ttcgcttaat gcgtatgcat
     5821 taattaacat actcggtggc ctccccacca ccaacttttt tgcactgcaa aaaaacacgc
     5881 ttttgcacgc gggcccatac atagtacaaa ctctacgttt cgtagactat tttacataaa
     5941 tagtctacac cgttgtatac gctccaaata cactaccaca cattgaacct ttttgcagtg
     6001 caaaaaagta cgtgtcggca gtcacgtagg ccggccttat cgggtcgcgt cctgtcacgt
     6061 acgaatcaca ttatcggacc ggacgagtgt tgtcttatcg tgacaggacg ccagcttcct
     6121 gtgttgctaa ccgcagccgg acgcaactcc ttatcggaac aggacgcgcc tccatatcag
     6181 ccgcgcgtta tctcatgcgc gtgaccggac acgaggcgcc cgtcccgctt atcgcgccta
     6241 taaatacagc ccgcaacgat ctggtaaaca cagttgaaca gcatctgtta cagcgacaca
     6301 acatgagccg gtccaacaac gccaacgcgc ccacgccatc caaccgccgc cgcaacctgt
     6361 ctctggtggc tagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6481 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     6541 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
     6601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     6841 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     6901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6961 tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc
     7021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7081 agctgtacaa gtaagcgctg actctagatc ataatcagcc ataccacatt tgtagaggtt
     7141 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7201 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7261 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7321 atcaatgtat cttaaagctt ccttaagttt ttgctcacct gtgattgctc ctactcaaat
     7381 acaaaaacat caaattttct gtcaataaag catatttatt tatatttatt ttacaggaaa
     7441 gaattccttt taaagtgtat tttaacctat aatgaaaaac gattaaaaaa aatacataaa
     7501 ataattcgaa aatttttgaa tagcccaggt tgataaaaat tcatttcata cgttttataa
     7561 cttatgcccc taagtatttt ttgaccatag tgtttcaatt ctacattaat tttacagagt
     7621 agaatgaaac gccacctact cagccaagag gcgaaaaggt tagctcgcca agcagagagg
     7681 gcgccagtgc tcactacttt ttataattct caacttcttt ttccagactc agttcgtata
     7741 tatagaccta ttttcaattt aacgtcgggc ggcagcggcg ggaatgggtt ttagagctag
     7801 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     7861 tgcttttttg cctacctgga gcctgagagt tgttcaataa aataaaaatg tttcgttttt
     7921 ttgctttcgc cagtatttat tatttttcat caatatgtat tcaatttggt atgtatttag
     7981 taattgtaat atatagacaa tggttttccg ttgacgtaca tacatctgac gtgtgtttat
     8041 ttagacataa tagttatgtt ttcacatctt tttaatgttc gcttaatgcg tatgcatctc
     8101 gagcctatct atgcaaatct cagtgtggga tgtaagccaa gttgagtact ttttcttgaa
     8161 atgattaaaa tttcttttgc aatatgtcta tttgcgggac aatctataac aaaaagtttc
     8221 gctttgtttt tttaaaacat cctcataagc atctgaaaca gctattgaag ctctaaatct
     8281 gtcaaatatt gcctgctgtt tctcacgctt tttctctcca gaactccaag tctcaaacaa
     8341 ttctgaattg gccaacctga ggttgtgacc taaaaatgta tattttagtg ttagttttta
     8401 ttgttaattt gtacacgtaa agcggcaatt aaaagcactc aacacaaaca aatccaactt
     8461 gtgacgacca caaacatata gtacgaaccg ctctctttcg atgccatgac aacttgtttg
     8521 atataaactt agattggtgc tttattttta ataattttaa acggactctt ttttccgcag
     8581 agtagctttc gtgcacccgt gataccgaac agctgtttta acagcctata tattttaaca
     8641 gatcgtgact cagataaaat ttctgttacg taacattgaa gtttgatttt aatattaacg
     8701 actttcaaaa aatggatttt tgcccacaaa acgtgatcag ccagcctaaa gtgcactcgc
     8761 tgcaaatagg accgcacgcg taatgtattt tagtattttc tacgagttaa attaattttt
     8821 gtatttgacg tttcgggatg tttttaaaaa aggtagttgt tgttgcggca gtacccagta
     8881 gccttccagc ggctcgaaac tgaacatttg atctcatcct gaaaagaaat attgctatgc
     8941 tcatttctaa gaaaatgacc aacctccatc ttgtgtcctg cttggcaaga ctttgcacct
     9001 gttgcaccaa aagtacatta ttttcactac gcttgcttga atagaattct catcggcgcg
     9061 cctttttgct cacctgtgat tgctcctact caaatacaaa aacatcaaat tttctgtcaa
     9121 taaagcatat ttatttatat ttattttaca ggaaagaatt ccttttaaag tgtattttaa
     9181 cctataatga aaaacgatta aaaaaaatac ataaaataat tcgaaaattt ttgaatagcc
     9241 caggttgata aaaattcatt tcatacgttt tataacttat gcccctaagt attttttgac
     9301 catagtgttt caattctaca ttaattttac agagtagaat gaaacgccac ctactcagcc
     9361 aagaggcgaa aaggttagct cgccaagcag agagggcgcc agtgctcact actttttata
     9421 attctcaact tctttttcca gactcagttc gtatatatag acctattttc aatttaacgt
     9481 cgcaatgcgg taaccagatc gggttttaga gctagaaata gcaagttaaa ataaggctag
     9541 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgcctac ctggagcctg
     9601 agagttgttc aataaaataa aaatgtttcg tttttttgct ttcgccagta tttattattt
     9661 ttcatcaata tgtattcaat ttggtatgta tttagtaatt gtaatatata gacaatggtt
     9721 ttccgttgac gtacatacat ctgacgtgtg tttatttaga cataatagtt atgttttcac
     9781 atctttttaa tgttcgctta atgcgtatgc atcctaggcc ggccgatctc ggatctgaca
     9841 atgttcagtg cagagactcg gctacgcctc gtggactttg aagttgacca acaatgttta
     9901 ttcttacctc taatagtcct ctgtggcaag gtcaagattc tgttagaagc caatgaagaa
     9961 cctggttgtt caataacatt ttgttcgtct aatatttcac taccgcttga cgttggctgc
    10021 acttcatgta cctcatctat aaacgcttct tctgtatcgc tctggacgtc atcttcactt
    10081 acgtgatctg atatttcact gtcagaatcc tcaccaacaa gctcgtcatc gctttgcaga
    10141 agagcagaga ggatatgctc atcgtctaaa gaactaccca ttttattata tattagtcac
    10201 gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata
    10261 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca
    10321 ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac
    10381 gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
    10441 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
    10501 tttctagggt taaaaaagat ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa
    10561 tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa
    10621 tggcaaacca agtcgcgcga gcgtcgactc tagaggatcc ccgggtaccg agctcgaatt
    10681 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10741 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10801 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10861 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10981 aaaggcggta atacggt
//
