LOCUS       sequence-36212-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer 
            AL, Baserga SJ
  TITLE     RNA polymerase I transcription and pre-rRNA processing are linked by
            specific SSU processome components.
  JOURNAL   Genes Dev. 2004 Oct 15. 18(20):2506-17.
  PUBMED    15489292
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Oct 15. 18(20):2506-17."
COMMENT     Sequence Label: pTOPO-rRNA-5'ETS 923 to 1179
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..37)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             356..367
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(394..412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(433..449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(446..468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(481..487,488..505,506..511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    526..547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(664..681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgattgat ttagcggccg cgaattcgcc cttcaatgtc ttcggactct ttgtgcgcag
       61 ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgttattt attgtcacca
      121 cctccctgaa ttaggattgg gtaatttgcg cgcctgctgc cttccttgga tgtggtagcc
      181 gtttctcagg ctccctctcc ggaatcgaac ccttattccc cgttacccgt tgaaaccatg
      241 gtaggccact atcctaccat cgaaagttga tagggcagaa atttgaatga accatcgcca
      301 gcacaaggcc atgcgattcg aaaagttatt atgaatcatc aaagagtccg aagacattga
      361 agggcgaatt cgtttaaacc tgcaggacta gtccctttag tgagggttaa ttctgagctt
      421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      901 cccgacagga ctataaag
//
