LOCUS       sequence-362126-        4413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A3_STOP
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1970..2069)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2086..2105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2087..2105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2110..2126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2110..2126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2228..2247)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2239..3048
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2306..2325)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3195..3783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3684..3703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3937..3954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4113..4140)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4232..4318)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4367..4389
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4381..4398
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4382..4398
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag caagtccaag
      121 tgcagcgtgg gactgatgag ctccgtggtg gcacctgcaa aggagccaaa cgcagtgggc
      181 cccaaggagg tggagctgat cctggtgaag gagcagaacg gcgtgcagct gacctctagc
      241 accctgacaa atcccaggca gtctcctgtg gaggcacagg acagggagac atggggcaag
      301 aagatcgatt tcctgctgag cgtgatcggc tttgccgtgg acctggccaa cgtgtggagg
      361 ttcccatacc tgtgctataa gaatggagga ggagccttcc tggtgccata cctgctgttc
      421 atggtcatcg ccggcatgcc cctgttctat atggagctgg ccctgggaca gtttaacagg
      481 gagggagcag caggcgtgtg gaagatctgt cctatcctga agggcgtggg cttcaccgtg
      541 atcctgatct ccctgtacgt gggcttcttt tataatgtga tcatcgcctg ggccctgcac
      601 tacctgttct cctcttttac cacagagctg ccttggatcc actgcaacaa ttcttggaac
      661 agcccaaatt gtagcgacgc acacccaggc gacagctccg gcgattctag cggcctgaat
      721 gatacattcg gcaccacacc agccgccgag tattttgaga ggggcgtgct gcacctgcac
      781 cagtcccacg gaatcgacga tctgggacca cctagatggc agctgaccgc atgcctggtg
      841 ctggtcatcg tgctgctgta cttctctctg tggaagggcg tgaagacaag cggcaaggtg
      901 gtgtggatca ccgccacaat gccatatgtg gtgctgaccg ccctgctgct gaggggagtg
      961 acactgccag gagcaatcga cggaatcagg gcatacctgt ccgtggactt ctacagactg
     1021 tgcgaggcaa gcgtgtggat cgacgcagca acccaggtgt gcttcagcct gggcgtgggc
     1081 tttggcgtgc tgatcgcctt ctcctcttac aacaagttta ccaacaactg ttacagagat
     1141 gccatcgtga ccacatccat caattctctg acaagcttca gctccggctt tgtggtgttc
     1201 agctttctgg gctacatggc ccagaagcac tccgtgccaa tcggcgacgt ggcaaaggat
     1261 ggacctggac tgatcttcat catctatcct gaggccatcg ccaccctgcc actgtctagc
     1321 gcctgggcag tggtgttctt tatcatgctg ctgaccctgg gaatcgacag cgccatggga
     1381 ggcatggagt ccgtgatcac aggcctgatc gatgagtttc agctgctgca ccggcacaga
     1441 gagctgttca ccctgtttat cgtgctggcc acattcctgc tgtccctgtt ttgcgtgacc
     1501 aacggcggca tctacgtgtt cacactgctg gaccactttg cagcaggcac ctccatcctg
     1561 ttcggcgtgc tgatcgaggc aatcggagtg gcatggttct acggagtggg acagttttct
     1621 gacgatatcc agcagatgac aggccagcgg ccctctctgt attggagact gtgctggaag
     1681 ctggtgagcc cttgtttcct gctgtttgtg gtggtggtgt ccatcgtgac cttcaggcca
     1741 ccccactacg gcgcctatat ctttcccgat tgggccaatg ccctgggctg ggtcatcgcc
     1801 acatcctcta tggccatggt gcctatctac gccgcctata agttctgttc cctgccaggc
     1861 tcttttaggg agaagctggc ctacgccatc gcccctgaga aggacaggga gctggtggat
     1921 aggggagagg tgcgccagtt caccctgaga cactggctga aggtgtaaga cccagctttc
     1981 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2041 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2101 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2161 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2221 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2281 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2341 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2401 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2461 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2521 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2581 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2641 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2701 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2761 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2821 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2881 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2941 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3001 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3061 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3121 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3301 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3901 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3961 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4021 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4081 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4141 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4201 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4261 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4321 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4381 tgtaaaacga cggccagtct taagctcggg ccc
//
