LOCUS       sequence-362130-        4143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC29A4_STOP
FEATURES             Location/Qualifiers
     source          1..4143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1700..1799)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1816..1835)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1817..1835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1840..1856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1840..1856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1958..1977)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1969..2778
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2036..2055)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2925..3513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3414..3433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3667..3684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3843..3870)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3962..4048)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4097..4119
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4111..4128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4112..4128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg ctccgtgggc
      121 tctcagcggc tggaggagcc atccgtggca ggaacacccg accctggcgt ggtcatgtcc
      181 ttcacctttg attctcacca gctggaggag gcagcagagg cagcacaggg acagggcctg
      241 agggcccgcg gcgtgcctgc cttcaccgac accacactgg atgagccagt gcccgacgat
      301 agataccacg ccatctattt tgcaatgctg ctggcaggag tgggcttcct gctgccttac
      361 aactctttta tcaccgacgt ggattacctg caccacaagt atccaggcac aagcatcgtg
      421 ttcgacatgt ccctgaccta tatcctggtg gccctggcag ccgtgctgct gaacaatgtg
      481 ctggtggaga ggctgaccct gcacacaagg atcaccgcag gatacctgct ggccctggga
      541 ccactgctgt tcatcagcat ctgcgacgtg tggctgcagc tgttttcccg ggatcaggcc
      601 tatgccatca atctggcagc agtgggaaca gtggccttcg gctgtaccgt gcagcagagc
      661 tccttttacg gctatacagg catgctgcct aagagataca cccagggcgt gatgacagga
      721 gagagcaccg caggcgtgat gatctctctg agcaggatcc tgacaaagct gctgctgcca
      781 gacgagcgcg cctctaccct gatcttcttt ctggtgagcg tggccctgga gctgctgtgc
      841 ttcctgctgc acctgctggt gcggagatct aggttcgtgc tgttttacac cacaaggcca
      901 agagatagcc acaggggccg ccctggactg ggaaggggat acggatatag ggtgcaccac
      961 gacgtggtgg caggcgatgt gcactttgag caccctgcac cagccctggc acctaacgag
     1021 tctccaaagg acagcccagc acacgaggtg acaggatccg gaggcgccta tatgaggttc
     1081 gatgtgccaa ggcctagagt gcagcggtct tggcccacct ttagagccct gctgctgcac
     1141 agatacgtgg tggcaagagt gatctgggcc gacatgctga gcatcgccgt gacatatttc
     1201 atcaccctgt gcctgtttcc cggcctggag tccgagatcc gccactgtat cctgggcgag
     1261 tggctgccta tcctgatcat ggccgtgttc aatctgagcg actttgtggg caagatcctg
     1321 gccgccctgc cagtggattg gaggggaacc cacctgctgg catgctcttg tctgagagtg
     1381 gtgttcatcc ccctgtttat cctgtgcgtg taccctagcg gcatgccagc cctgagacac
     1441 ccagcatggc catgtatctt ctctctgctg atgggcatca gcaacggcta ttttggatcc
     1501 gtgccaatga tcctggcagc aggcaaggtg tccccaaagc agagggagct ggcaggcaat
     1561 accatgacag tgagctacat gtccggactg acactgggat ccgccgtggc ctactgcaca
     1621 tattctctga ccagggatgc acacggaagc tgtctgcacg catccaccgc aaacggatct
     1681 atcctggcag gactgtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     1741 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1801 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1861 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1921 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     1981 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2041 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2101 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2161 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2221 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2281 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2341 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2401 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2461 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2521 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2581 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2641 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2701 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     2761 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     2821 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     2881 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2941 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3001 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3061 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3541 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3601 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3661 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3721 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3781 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3841 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     3901 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     3961 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4021 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4081 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4141 ccc
//
