LOCUS       sequence-362139-        9772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SuperFi-Cas9 PEmax prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Kulcsar PI, Talas A, Ligeti Z, Krausz SL, Welker E
  TITLE     SuperFi-Cas9 exhibits remarkable fidelity but severely reduced 
            activity yet works effectively with ABE8e.
  JOURNAL   Nat Commun. 2022 Nov 11;13(1):6858. doi: 10.1038/s41467-022-34527-8.
  PUBMED    36369279
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-11"; volume: "13(1):6858. doi"; pages: " 
            10.1038/s41467-022-34527-8"
COMMENT     Sequence Label: pAT15547_PEmax-SuperFi-Cas9
FEATURES             Location/Qualifiers
     source          1..9772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             380..400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             410..436
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(462..479)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    468..692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(763..779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(763..779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(776..798)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    787..803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(811..817,818..835,836..841))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    856..877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(994..1011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1165..1753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1245..1264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1924..2715,2716..2784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2547..2566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2785..2889)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2968..2987)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3159..3538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3539..3742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3693..3713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3784..3803
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3784..3802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3852..3872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3873..7973
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVDGDDKVDDDDKMIAKSEDEIGDATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8031..8051
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacccaggcc ctgaagatgg ccgagggcaa gaagctgaac gtgtacacag actccagata
       61 tgccttcgcc accgcacaca tccacggaga gatctacagg cgccggggct ggctgacctc
      121 tgagggcaag gagatcaaga acaaggatga gatcctggcc ctgctgaagg ccctgtttct
      181 gcccaagcgg ctgagcatca tccactgtcc tggacaccag aagggacact ccgccgaggc
      241 aaggggcaat cggatggccg accaggccgc cagaaaggct gctattactg aaactcccga
      301 cacttccact ctgctgattg aaaactcctc cccttctggc ggctcaaaaa gaaccgccga
      361 cggcagcgaa ttcgagtctc ccaagaagaa gaggaaagtc ggctctggcc ctgccgctaa
      421 gagagtgaag ctggactaac cggtttaaac ccgctgatca gcctcgactg tgccttctag
      481 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      541 tcccactgtc ctttcctaat aaaatgagaa aattgcatcg cattgtctga gtaggtgtca
      601 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      661 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
      721 ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
      781 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      841 agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      961 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1021 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1081 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc agtggaacga
     1801 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1861 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2881 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc
     2941 tcccgatccc ctagggtcta ctctcagtac aatctgctct gatgccgcat agttaagcca
     3001 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     3061 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     3121 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     3181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3361 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3541 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3601 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     3661 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     3721 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctaga gatccgcggc
     3781 cgctaatacg actcactata gggagagccg ccaccatgaa acggacagcc gacggaagcg
     3841 agttcgagtc accaaagaag aagcggaaag tcgacaagaa gtacagcatc ggcctggaca
     3901 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     3961 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     4021 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     4081 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     4141 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     4201 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     4261 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     4321 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     4381 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     4441 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     4501 tcctgtctgc cagactgagc aagagcagaa agctggaaaa tctgatcgcc cagctgcccg
     4561 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     4621 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     4681 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     4741 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     4801 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     4861 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     4921 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     4981 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     5041 tgaagctgaa gagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     5101 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     5161 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     5221 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     5281 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     5341 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     5401 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     5461 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     5521 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     5581 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     5641 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     5701 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     5761 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     5821 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     5881 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     5941 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     6001 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     6061 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     6121 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     6181 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     6241 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     6301 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     6361 accggctgtc cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact
     6421 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     6481 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     6541 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     6601 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     6661 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     6721 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     6781 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcg tacctgaacg
     6841 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtggacg
     6901 gcgacgacaa ggtggacgac gacgacaaga tgatcgccaa gagcgaagac gaaatcggcg
     6961 acgctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     7021 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     7081 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     7141 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     7201 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     7261 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     7321 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     7381 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     7441 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     7501 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     7561 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     7621 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     7681 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     7741 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     7801 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     7861 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     7921 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt gactccggcg
     7981 gaagctctgg tggcagcaag cggaccgccg acggctctga attcgagagc cctaagaaga
     8041 aaagaaaggt gagcggaggc tctagcggcg gaagcaccct gaacattgaa gacgagtata
     8101 gactgcatga aacaagcaag gaacccgacg tgtccctggg ctccacctgg ctgtccgact
     8161 ttccccaggc ctgggccgag acaggaggaa tgggcctggc cgtgcggcag gcacccctga
     8221 tcatccctct gaaggccacc tctacacccg tgagcatcaa gcagtaccct atgtctcagg
     8281 aggccagact gggcatcaag cctcacatcc agaggctgct ggaccagggc atcctggtgc
     8341 catgccagag cccctggaac acaccactgc tgcccgtgaa gaagccaggc accaatgact
     8401 atagacccgt gcaggatctg agagaggtga acaagagggt ggaggatatc caccccaccg
     8461 tgcccaaccc ttacaatctg ctgtccggcc tgcccccttc tcaccagtgg tatacagtgc
     8521 tggacctgaa ggatgccttc ttttgtctga gactgcaccc taccagccag ccactgttcg
     8581 cctttgagtg gagggaccct gagatgggca tctctggcca gctgacctgg acacgcctgc
     8641 ctcagggctt caagaatagc ccaacactgt ttaacgaggc cctgcaccgc gacctggcag
     8701 atttccggat ccagcaccca gatctgatcc tgctgcagta cgtggacgat ctgctgctgg
     8761 ccgccaccag cgagctggat tgccagcagg gaacacgcgc cctgctgcag accctgggaa
     8821 acctgggata tagggcatcc gccaagaagg cccagatctg tcagaagcag gtgaagtacc
     8881 tgggctatct gctgaaggag ggccagagat ggctgacaga ggccaggaag gagacagtga
     8941 tgggccagcc aacacccaag accccaagac agctgaggga gttcctgggc aaagcaggat
     9001 tttgcaggct gttcatccca ggattcgcag agatggcagc acctctgtac ccactgacca
     9061 agccgggcac cctgtttaat tggggccctg accagcagaa ggcctatcag gagatcaagc
     9121 aggccctgct gacagcacca gccctgggcc tgccagacct gaccaagcct ttcgagctgt
     9181 ttgtggatga gaagcagggc tacgccaagg gcgtgctgac ccagaagctg ggaccatgga
     9241 gacggcccgt ggcctatctg tccaagaagc tggacccagt ggcagcagga tggccaccat
     9301 gcctgaggat ggtggcagca atcgccgtgc tgacaaagga tgccggcaag ctgaccatgg
     9361 gacagccact ggtcatcctg gcaccacacg cagtggaggc cctggtgaag cagcctccag
     9421 atcgctggct gtctaacgcc cggatgacac actaccaggc cctgctgctg gacaccgatc
     9481 gcgtgcagtt tggccctgtg gtggccctga atccagccac cctgctgcct ctgccagagg
     9541 agggcctgca gcacaactgt ctggacatcc tggcagaggc acacggaaca aggccagacc
     9601 tgaccgatca gcccctgcct gacgccgatc acacatggta taccgatgga agctccctgc
     9661 tgcaggaggg ccagaggaag gcaggagcag cagtgaccac agagacagaa gtgatctggg
     9721 ccaaggccct gccagcaggc acatccgccc agcgggccga gctgatcgcc ct
//
