LOCUS       sequence-36215-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     RB shRNAs
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSLIK sh human Rb 1534 hyg
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           44..165
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           193..207
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           235..361
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(400..424)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        474..876
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ctgtacaagt aaactagtcg actagggata acagggtaat tgtttgaatg aggcttcagt
       61 actttacaga atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcttcaggt
      121 taacccaaca gaaggctcga gaaggtatat tgctgttgac agtgagcgaa agaacgatta
      181 tccattcaaa tagtgaagcc acagatgtat ttgaatggat aatcgttctt ctgcctactg
      241 cctcggaatt caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct
      301 tgctatctct ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt
      361 ttttcaattg gaagactaat gcgttttcga gatatctaga cccagctttc ttgtacaaag
      421 tggttgataa catctcgagc cataaagatg gttaattaac ccacccaaga tctggcctcc
      481 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
      541 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
      601 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
      661 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
      721 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaaacgc cgatgattat
      781 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt ggggtcgcgg
      841 ttcttggttt gtggatcgct gtgatcgtca cttggtgagt aagcggggct gctgggctgg
      901 ccggggctt
//
