LOCUS       sequence-362150-       10350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP and human SOX2 under the human GFAP ABC1D 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Niu W, Zang T, Zou Y, Fang S, Smith DK, Bachoo R, Zhang CL
  TITLE     In vivo reprogramming of astrocytes to neuroblasts in the adult 
            brain.
  JOURNAL   Nat Cell Biol. 2013 Oct;15(10):1164-75. doi: 10.1038/ncb2843. Epub 
            2013 Sep 22.
  PUBMED    24056302
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2843"; 
            journalName: "Nat Cell Biol"; date: "2013-10"; volume: "15"; issue: 
            "10"; pages: "1164-75"
COMMENT     Sequence Label: LV-hGFAP-GFP-T2A-SOX2
FEATURES             Location/Qualifiers
     source          1..10350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      285..294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(291..293,294..296,297..1007)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(291..293,294..296,297..1007)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(336..357)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(597..616)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     944..965
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1026..1079
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    2060..2648
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2113..2133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2531..2542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3167..3347
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3373..3390)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3379..3603
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3649..4077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3736..3755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3946..3967
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4086..4106)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4091..4420
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4271..4406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4333..4352
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4468..4515
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4534..4908
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5038..5159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5075..5094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5129..5148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5208..5224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5208..5224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5221..5243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5232..5248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5256..5262,5263..5280,5281..5286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5301..5322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5439..5456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5610..6198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5690..5709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6369..7160,7161..7229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7230..7334)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7409..7428)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7600..7979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7981..8179
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8134..8154
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8197..8377
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8424..8549
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9042..9275
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9460..9504
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9778..9895
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgcccaggc atggacagtg ggtggcaggg ggggagagga gggctgtctg cttcccagaa
       61 gtccaaggac acaaatgggt gaggggagag ctctccccat agctgggctg cggcccaacc
      121 ccaccccctc aggctatgcc agggggtgtt gccaggggca cccgggcatc gccagtctag
      181 cccactcctt cataaagccc tcgcatccca ggagcgagca gagccagagc aggttggaga
      241 ggagacgcat cacctccgct gctcgcaccg ggctctagac ccgggccacc atggtgagca
      301 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      361 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      421 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      481 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      541 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      601 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      661 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      721 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      781 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      841 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      901 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      961 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     1021 cctacgaggg gagaggaagt cttctgacct gcggagacgt cgaagagaat cctggacccg
     1081 ctagctacaa catgatggag acggagctga agccgccggg cccgcagcaa acttcggggg
     1141 gcggcggcgg caactccacc gcggcggcgg ccggcggcaa ccagaaaaac agcccggacc
     1201 gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg cgggcagcgg cgcaagatgg
     1261 cccaggagaa ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gccgagtgga
     1321 aacttttgtc ggagacggag aagcggccgt tcatcgacga ggctaagcgg ctgcgagcgc
     1381 tgcacatgaa ggagcacccg gattataaat accggccccg gcggaaaacc aagacgctca
     1441 tgaagaagga taagtacacg ctgcccggcg ggctgctggc ccccggcggc aatagcatgg
     1501 cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt gaaccagcgc atggacagtt
     1561 acgcgcacat gaacggctgg agcaacggca gctacagcat gatgcaggac cagctgggct
     1621 acccgcagca cccgggcctc aatgcgcacg gcgcagcgca gatgcagccc atgcaccgct
     1681 acgacgtgag cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct
     1741 cgcccaccta cagcatgtcc tactcgcagc agggcacccc tggcatggct cttggctcca
     1801 tgggttcggt ggtcaagtcc gaggccagct ccagcccccc tgtggttacc tcttcctccc
     1861 actccagggc gccctgccag gccggggacc tccgggacat gatcagcatg tatctccccg
     1921 gcgccgaggt gccggaaccc gccgccccca gcagacttca catgtcccag cactaccaga
     1981 gcggcccggt gcccggcacg gccattaacg gcacactgcc cctctcacac atgtgactcg
     2041 agggatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2101 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2161 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2221 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2281 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2341 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2401 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     2461 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2521 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2581 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2641 ctccccgcat cgataccgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     2701 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     2761 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     2821 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     2881 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     2941 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     3001 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     3061 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     3121 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     3181 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     3241 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3301 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     3361 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3421 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3481 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3541 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3601 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     3661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3781 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4021 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4081 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4141 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4321 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4381 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4441 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     4501 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     4561 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     4621 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     4681 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     4741 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     4801 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     4861 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     4921 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4981 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5041 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5161 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7261 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7321 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     7381 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7441 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     7501 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     7561 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     7621 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     7681 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7741 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     7801 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     7861 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7921 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7981 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     8041 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     8101 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     8161 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     8221 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8281 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8341 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     8401 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8461 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8521 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8581 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8641 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8701 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8761 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8821 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8881 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     8941 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9001 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9061 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9121 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9181 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9241 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9301 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9361 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9421 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9481 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9541 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9601 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9661 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     9721 agagacagat ccattcgatt agtgaacgga tctacaaatg gcagtattca tccacaattt
     9781 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9841 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9901 ttattacagg gacagcagaa attcactttg aattcaacat atcctggtgt ggagtagggg
     9961 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
    10021 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
    10081 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
    10141 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
    10201 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
    10261 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
    10321 cagcctgtgc ccaggaaagg ggatcagggg
//
