LOCUS       sequence-362153-       10853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human SOX2 and GFP under the human GFAP ABC1D 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10853)
  TITLE     GFAP ABC1D promoter plasmids
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LV-hGFAP-SOX2-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    259..826
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    277..828
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(421..438)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     648..667
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(831..833,834..836,837..1550)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(876..897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1137..1156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1484..1505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1574..2162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1627..1647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2045..2056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2681..2861
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2887..2904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2893..3117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3163..3591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3250..3269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3460..3481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3600..3620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3605..3934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3785..3920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3847..3866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3982..4029
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4048..4422
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4552..4673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4589..4608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4643..4662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4722..4738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4735..4757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4746..4762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4770..4776,4777..4794,4795..4800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4815..4836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4953..4970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5124..5712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5204..5223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5883..6674,6675..6743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6744..6848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6923..6942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7114..7493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7495..7693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7648..7668
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7711..7891
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7938..8063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8556..8789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8974..9018
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9292..9409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccatgg gttcggtggt caagtccgag gccagctcca gcccccctgt ggttacctct
       61 tcctcccact ccagggcgcc ctgccaggcc ggggacctcc gggacatgat cagcatgtat
      121 ctccccggcg ccgaggtgcc ggaacccgcc gcccccagca gacttcacat gtcccagcac
      181 taccagagcg gcccggtgcc cggcacggcc attaacggca cactgcccct ctcacacatg
      241 tgactcgaga tgcagaattc cgnccccccc cccctaacgt tactggccga agccgcttgg
      301 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
      361 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
      421 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
      481 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
      541 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
      601 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
      661 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
      721 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc
      781 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gtcgagggat
     1561 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     1621 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     1681 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     1741 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     1801 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     1861 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     1921 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     1981 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2041 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2101 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2161 gcatcgatac cgtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     2221 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     2281 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2341 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     2401 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     2461 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     2521 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     2581 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     2641 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     2701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2821 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     2881 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2941 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3001 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3061 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3121 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3361 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3421 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3481 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3541 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3601 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3661 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3841 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3901 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     3961 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     4021 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4381 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     4441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4501 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4681 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4741 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4861 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6841 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6901 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6961 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7021 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7081 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7141 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7201 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7261 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7321 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7381 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7441 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7501 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7561 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7621 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7681 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7741 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7801 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7861 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7921 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7981 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8041 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8101 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8161 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8221 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8281 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8341 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8401 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8461 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8521 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8581 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8641 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8701 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8761 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8821 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8881 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8941 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9001 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9061 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9121 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9181 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     9241 agatccattc gattagtgaa cggatctaca aatggcagta ttcatccaca attttaaaag
     9301 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9361 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     9421 cagggacagc agaaattcac tttgaattca acatatcctg gtgtggagta ggggacgctg
     9481 ctctgacaga ggctcggggg cctgagctgg ctctgtgagc tggggaggag gcagacagcc
     9541 aggccttgtc tgcaagcaga cctggcagca ttgggctggc cgccccccag ggcctcctct
     9601 tcatgcccag tgaatgactc accttggcac agacacaatg ttcggggtgg gcacagtgcc
     9661 tgcttcccgc cgcaccccag cccccctcaa atgccttccg agaagcccat tgagcagggg
     9721 gcttgcattg caccccagcc tgacagcctg gcatcttggg ataaaagcag cacagccccc
     9781 taggggctgc ccttgctgtg tggcgccacc ggcggtggag aacaaggctc tattcagcct
     9841 gtgcccagga aaggggatca ggggatgccc aggcatggac agtgggtggc agggggggag
     9901 aggagggctg tctgcttccc agaagtccaa ggacacaaat gggtgagggg agagctctcc
     9961 ccatagctgg gctgcggccc aaccccaccc cctcaggcta tgccaggggg tgttgccagg
    10021 ggcacccggg catcgccagt ctagcccact ccttcataaa gccctcgcat cccaggagcg
    10081 agcagagcca gagcaggttg gagaggagac gcatcacctc cgctgctcgc accggtgcca
    10141 ccatgtacaa catgatggag acggagctga agccgccggg cccgcagcaa acttcggggg
    10201 gcggcggcgg caactccacc gcggcggcgg ccggcggcaa ccagaaaaac agcccggacc
    10261 gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg cgggcagcgg cgcaagatgg
    10321 cccaggagaa ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gccgagtgga
    10381 aacttttgtc ggagacggag aagcggccgt tcatcgacga ggctaagcgg ctgcgagcgc
    10441 tgcacatgaa ggagcacccg gattataaat accggccccg gcggaaaacc aagacgctca
    10501 tgaagaagga taagtacacg ctgcccggcg ggctgctggc ccccggcggc aatagcatgg
    10561 cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt gaaccagcgc atggacagtt
    10621 acgcgcacat gaacggctgg agcaacggca gctacagcat gatgcaggac cagctgggct
    10681 acccgcagca cccgggcctc aatgcgcacg gcgcagcgca gatgcagccc atgcaccgct
    10741 acgacgtgag cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct
    10801 cgcccaccta cagcatgtcc tactcgcagc agggcacccc tggcatggct ctt
//
