LOCUS       sequence-362155-        9337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP under the human GFAP ABC1D promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Niu W, Zang T, Zou Y, Fang S, Smith DK, Bachoo R, Zhang CL
  TITLE     In vivo reprogramming of astrocytes to neuroblasts in the adult 
            brain.
  JOURNAL   Nat Cell Biol. 2013 Oct;15(10):1164-75. doi: 10.1038/ncb2843. Epub 
            2013 Sep 22.
  PUBMED    24056302
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2843"; 
            journalName: "Nat Cell Biol"; date: "2013-10"; volume: "15"; issue: 
            "10"; pages: "1164-75"
COMMENT     Sequence Label: LV-hGFAP-GFP
FEATURES             Location/Qualifiers
     source          1..9337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..130
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    404..521
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      1250..1259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1256..1258,1259..1261,1262..1972)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1256..1258,1259..1261,1262..1972)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1301..1322)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1562..1581)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1909..1930
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2023..2611
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2076..2096)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2494..2505)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3130..3310
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3336..3353)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3342..3566
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3612..4040
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3699..3718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3909..3930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4049..4069)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4054..4383
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4234..4369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4296..4315
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4431..4478
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4497..4871
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5001..5122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5038..5057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5092..5111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5171..5187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5171..5187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5184..5206)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5195..5211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5219..5225,5226..5243,5244..5249))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5264..5285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5402..5419)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5573..6161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5653..5672)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6332..7123,7124..7192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7193..7297)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7372..7391)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7563..7942
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7944..8142
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8097..8117
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8160..8340
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8387..8512
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9005..9238
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
       61 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
      121 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
      181 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
      241 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
      301 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
      361 tcgattagtg aacggatcta caaatggcag tattcatcca caattttaaa agaaaagggg
      421 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      481 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
      541 gcagaaattc actttgaatt caacatatcc tggtgtggag taggggacgc tgctctgaca
      601 gaggctcggg ggcctgagct ggctctgtga gctggggagg aggcagacag ccaggccttg
      661 tctgcaagca gacctggcag cattgggctg gccgcccccc agggcctcct cttcatgccc
      721 agtgaatgac tcaccttggc acagacacaa tgttcggggt gggcacagtg cctgcttccc
      781 gccgcacccc agcccccctc aaatgccttc cgagaagccc attgagcagg gggcttgcat
      841 tgcaccccag cctgacagcc tggcatcttg ggataaaagc agcacagccc cctaggggct
      901 gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc tctattcagc ctgtgcccag
      961 gaaaggggat caggggatgc ccaggcatgg acagtgggtg gcaggggggg agaggagggc
     1021 tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg ggagagctct ccccatagct
     1081 gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca ggggcacccg
     1141 ggcatcgcca gtctagccca ctccttcata aagccctcgc atcccaggag cgagcagagc
     1201 cagagcaggt tggagaggag acgcatcacc tccgctgctc gcaccggtcg ccaccatggt
     1261 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1321 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1381 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1441 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1501 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1561 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1621 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1681 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1741 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1801 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1861 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1921 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     1981 cagatcctac tagtaggatc tcgagggatc aagcttatcg ataatcaacc tctggattac
     2041 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2101 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2161 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2221 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2281 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2341 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2401 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     2461 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2521 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2581 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgagacct agaaaaacat
     2641 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     2701 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     2761 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2821 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     2881 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     2941 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     3001 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     3061 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     3121 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3181 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3241 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3301 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3361 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3421 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3481 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3541 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3601 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4021 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4201 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4321 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4381 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     4441 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     4501 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     4561 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     4621 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     4681 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     4741 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     4801 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     4861 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4921 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4981 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5041 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5101 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5161 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5221 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5281 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5341 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5401 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5461 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5521 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5581 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5641 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5701 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5761 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5821 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5881 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5941 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6001 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6061 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6121 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6181 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6241 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6301 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6361 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6421 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6481 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6541 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6601 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6661 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6721 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6781 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6841 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6901 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6961 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7321 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     7381 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     7441 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     7501 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     7561 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     7621 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     7681 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     7741 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     7801 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     7861 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     7921 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     7981 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     8041 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     8101 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     8161 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8221 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8281 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8341 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     8401 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     8461 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     8521 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     8581 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     8641 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     8701 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     8761 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     8821 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     8881 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8941 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9001 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     9061 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9121 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9181 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9241 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     9301 ggagtaataa atctctggaa cagatttgga atcacac
//
