LOCUS       sequence-36225-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Rowell JJ, Mallik AK, Dugas-Ford J, Ragsdale CW
  TITLE     Molecular analysis of neocortical layer structure in the ferret.
  JOURNAL   J Comp Neurol. 2010 Aug 15. 518(16):3272-89.
  PUBMED    20575059
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Comp 
            Neurol. 2010 Aug 15. 518(16):3272-89."
COMMENT     Sequence Label: pfVGLUT1
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             424..435
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(497..516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(498..516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(523..540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(523..539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(532..554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(767..786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt cagcatagga
       61 accacaaaag gctgttgttg ccaagcgact ccgctctaag ggaggggccc atttgctcca
      121 gatgccgtgg caggcagggt acgtgacccc ctctaccaac ccctgcagga tcctcacgaa
      181 gatgacacag ccataatgga ctcgggcagc agaggggatc agcatgttta gagtggatgt
      241 agcgacaatg gcaaagccga aaaccctgtt ggctgcgaat ttttggcaga taaaccctcc
      301 gggaatctgg gtgacaatgt agccccagaa aaaggagccg tgtatgaggc caacagtctc
      361 tggatcccag ttaaattgag ctttctgcac caccacgtgg cctccgcggt gggtcgtgct
      421 gttattgaag ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
      481 tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      541 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      601 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      661 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      901 gatggttcac gtagtgggcc atcgccctga tagacggttt t
//
