LOCUS       sequence-362308-        9470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exogenous COX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, 
            Terai K
  TITLE     Calcium transients trigger switch-like discharge of prostaglandin 
            E(2) in an extracellular signal-regulated kinase-dependent manner.
  JOURNAL   eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727.
  PUBMED    38276879
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-01-26"; volume: "12:RP86727. doi"; pages: " 
            10.7554/eLife.86727"
COMMENT     Sequence Label: pPBpuro-COX2-EBFP2
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(4518..4520,4521..5231)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKV
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     primer_bind     complement(4560..4581)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5376..5925
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5520..5537)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5747..5766
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5928..6527
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5928..5947)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6424..6444
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6554..6571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6560..6784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6831..6847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6855..6871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6879..6885,6886..6903,6904..6909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6924..6945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7179..7241
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7282..7302)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7282..7300)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7321..7337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7345..7361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7369..7375,7376..7393,7394..7399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7414..7435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7552..7569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7723..8311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7803..7822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8482..9273,9274..9342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9105..9124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9343..9447)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs ggggsgrggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc gccaccatgc
     2761 tcgcccgcgc cctgctgctg tgcgcggtcc tggcgctcag ccatacagca aatccttgct
     2821 gttcccaccc atgtcaaaac cgaggtgtat gtatgagtgt gggatttgac cagtataagt
     2881 gcgattgtac ccggacagga ttctatggag aaaactgctc aacaccggaa tttttgacaa
     2941 gaataaaatt atttctgaaa cccactccaa acacagtgca ctacatactt acccacttca
     3001 agggattttg gaacgttgtg aataacattc ccttccttcg aaatgcaatt atgagttatg
     3061 tgttgacatc cagatcacat ttgattgaca gtccaccaac ttacaatgct gactatggct
     3121 acaaaagctg ggaagccttc tctaacctct cctattatac tagagccctt cctcctgtgc
     3181 ctgatgattg cccgactccc ttgggtgtca aaggtaaaaa gcagcttcct gattcaaatg
     3241 agattgtgga aaaattgctt ctaagaagaa agttcatccc tgatccccag ggctcaaaca
     3301 tgatgtttgc attctttgcc cagcacttca cgcatcagtt tttcaagaca gatcataagc
     3361 gagggccagc tttcaccaac gggctgggcc atggggtgga cttaaatcat atttacggtg
     3421 aaactctggc tagacagcgt aaactgcgcc ttttcaagga tggaaaaatg aaatatcaga
     3481 taattgatgg agagatgtat cctcccacag tcaaagatac tcaggcagag atgatctacc
     3541 ctcctcaagt ccctgagcat ctacggtttg ctgtggggca ggaggtcttt ggtctggtgc
     3601 ctggtctgat gatgtatgcc acaatctggc tgcgggaaca caacagagta tgcgatgtgc
     3661 ttaaacagga gcatcctgaa tggggtgatg agcagttgtt ccagacaagc aggctaatac
     3721 tgataggaga gactattaag attgtgattg aagattatgt gcaacacttg agtggctatc
     3781 acttcaaact gaaatttgac ccagaactac ttttcaacaa acaattccag taccaaaatc
     3841 gtattgctgc tgaatttaac accctctatc actggcatcc ccttctgcct gacacctttc
     3901 aaattcatga ccagaaatac aactatcaac agtttatcta caacaactct atattgctgg
     3961 aacatggaat tacccagttt gttgaatcat tcaccaggca aattgctggc agggttgctg
     4021 gtggtaggaa tgttccaccc gcagtacaga aagtatcaca ggcttccatt gaccagagca
     4081 ggcagatgaa ataccagtct tttaatgagt accgcaaacg ctttatgctg aagccctatg
     4141 aatcatttga agaacttaca ggagaaaagg aaatgtctgc agagttggaa gcactctatg
     4201 gtgacatcga tgctgtggag ctgtatcctg cccttctggt agaaaagcct cggccagatg
     4261 ccatctttgg tgaaaccatg gtagaagttg gagcaccatt ctccttgaaa ggacttatgg
     4321 gtaatgttat atgttctcct gcctactgga agccaagcac ttttggtgga gaagtgggtt
     4381 ttcaaatcat caacactgcc tcaattcagt ctctcatctg caataacgtg aagggctgtc
     4441 cctttacttc attcagtgtt ccagatccag agctcattaa aacagtcacc atcaatgcaa
     4501 gttcttcccg ctccggagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4561 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgaggggc gagggcgagg
     4621 gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4681 tgccctggcc caccctcgtg accaccctga gccacggcgt gcagtgcttc gcccgctacc
     4741 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4801 agcgcaccat cttcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg
     4861 agggcgacac cctggtgaac cgcatcgagc tgaagggcgt tgacttcaag gaggacggca
     4921 acatcctggg gcacaagctg gagtacaact tcaacagcca caacatctat atcatggccg
     4981 tcaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacgtg gaggacggca
     5041 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5101 tgcccgacag ccactacctg agcacccagt ccgtgctgag caaagacccc aacgagaagc
     5161 gcgatcacat ggtcctgctg gagttccgca ccgccgccgg gatcactctc ggcatggacg
     5221 agctgtacaa gtccggacta gatgatatca atcccacagt actactaaaa gaacgttcga
     5281 ctgaactgta actcgagggc ggccgctcta gagtcaacta acttaagcta gcaacggttt
     5341 ccctctagcg ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga
     5401 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5461 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5521 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5581 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5641 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5701 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     5761 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5821 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     5881 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg accgagtaca
     5941 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6001 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6061 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6121 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6181 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6241 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6301 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6361 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6421 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6481 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatcg atgatctaga
     6541 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6721 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6781 atggcttctg aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     6841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6901 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6961 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcttaaaagt tttgttactt
     7021 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     7081 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     7141 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     7201 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaatctag tatacgcgtt
     7261 taattaatcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     7321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7381 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8161 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
