LOCUS       sequence-362309-        9735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exogenous cPLA2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, 
            Terai K
  TITLE     Calcium transients trigger switch-like discharge of prostaglandin 
            E(2) in an extracellular signal-regulated kinase-dependent manner.
  JOURNAL   eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727.
  PUBMED    38276879
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-01-26"; volume: "12:RP86727. doi"; pages: " 
            10.7554/eLife.86727"
COMMENT     Sequence Label: pPBbsr2-cPLA2-mEGFP
FEATURES             Location/Qualifiers
     source          1..9735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(4998..5000,5001..5003,5004..5717)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5043..5064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5304..5323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5651..5672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5799..6348
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5943..5960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6170..6189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6819..6836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6825..7049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7096..7112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7120..7136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7144..7150,7151..7168,7169..7174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7189..7210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7444..7506
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7547..7567)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7547..7565)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7586..7602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7610..7626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7634..7640,7641..7658,7659..7664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7679..7700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7817..7834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7988..8576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8068..8087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8747..9538,9539..9607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9370..9389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9608..9712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs ggggsgrggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattcac catgtcattt atagatcctt
     2761 accagcacat tatagtggag caccagtatt cccacaagtt tacggtagtg gtgttacgtg
     2821 ccaccaaagt gacaaagggg gcctttggtg acatgcttga tactccagat ccctatgtgg
     2881 aactttttat ctctacaacc cctgacagca ggaagagaac aagacatttt aataatgaca
     2941 taaaccctgt gtggaatgag acctttgaat ttattttgga tcctaatcag gaaaatgttt
     3001 tggagattac gttaatggat gccaattatg tcatggatga aactctaggg acagcaacat
     3061 ttactgtatc ttctatgaag gtgggagaaa agaaagaagt tccttttatt ttcaaccaag
     3121 tcactgaaat ggttctagaa atgtctcttg aagtttgctc atgcccagac ctacgattta
     3181 gtatggctct gtgtgatcag gagaagactt tcagacaaca gagaaaagaa cacataaggg
     3241 agagcatgaa gaaactcttg ggtccaaaga atagtgaagg attgcattct gcacgtgatg
     3301 tgcctgtggt agccatattg ggttcaggtg ggggtttccg agccatggtg ggattctctg
     3361 gtgtgatgaa ggcattatac gaatcaggaa ttctggattg tgctacctac gttgctggtc
     3421 tttctggctc cacctggtat atgtcaacct tgtattctca ccctgatttt ccagagaaag
     3481 ggccagagga gattaatgaa gaactaatga aaaatgttag ccacaatccc cttttacttc
     3541 tcacaccaca gaaagttaaa agatatgttg agtctttatg gaagaagaaa agctctggac
     3601 aacctgtcac ctttactgat atctttggga tgttaatagg agaaacacta attcataata
     3661 gaatgaatac tactctgagc agtttgaagg aaaaagttaa tactgcacaa tgccctttac
     3721 ctcttttcac ctgtcttcat gtcaaacctg acgtttcaga gctgatgttt gcagattggg
     3781 ttgaatttag tccatacgaa attggcatgg ctaaatatgg tacttttatg gctcccgact
     3841 tatttggaag caaatttttt atgggaacag tcgttaagaa gtatgaagaa aaccccttgc
     3901 atttcttaat gggtgtctgg ggcagtgcct tttccatatt gttcaacaga gttttgggcg
     3961 tttctggttc acaaagcaga ggctccacaa tggaggaaga attagaaaat attaccacaa
     4021 agcatattgt gagtaatgat agctcggaca gtgatgatga atcacacgaa cccaaaggca
     4081 ctgaaaatga agatgctgga agtgactatc aaagtgataa tcaagcaagt tggattcatc
     4141 gtatgataat ggccttggtg agtgattcag ctttattcaa taccagagaa ggacgtgctg
     4201 ggaaggtaca caacttcatg ctgggcttga atctcaatac atcttatcca ctgtctcctt
     4261 tgagtgactt tgccacacag gactcctttg atgatgatga actggatgca gctgtagcag
     4321 atcctgatga atttgagcga atatatgagc ctctggatgt caaaagtaaa aagattcatg
     4381 tagtggacag tgggctcaca tttaacctgc cgtatccctt gatactgaga cctcagagag
     4441 gggttgatct cataatctcc tttgactttt ctgcaaggcc aagtgactct agtcctccgt
     4501 tcaaggaact tctacttgca gaaaagtggg ctaaaatgaa caagctcccc tttccaaaga
     4561 ttgatcctta tgtgtttgat cgggaagggc tgaaggagtg ctatgtcttt aaacccaaga
     4621 atcctgatat ggagaaagat tgcccaacca tcatccactt tgttctggcc aacatcaact
     4681 tcagaaagta caaggctcca ggtgttccaa gggaaactga ggaagagaaa gaaattgctg
     4741 actttgatat ttttgatgac ccagaatcac cattttcaac cttcaatttt caatatccaa
     4801 atcaagcatt caaaagacta catgatctta tgcacttcaa tactctgaac aacattgatg
     4861 tgataaaaga agccatggtt gaaagcattg aatatagaag acagaatcca tctcgttgct
     4921 ctgtttccct tagtaatgtt gaggcaagaa gatttttcaa caaggagttt ctaagtaaac
     4981 ccaaagcaaa attcggcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5041 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     5101 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     5161 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     5221 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5281 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5341 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5401 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     5461 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     5521 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5581 tgctgcccga caaccactac ctgagcaccc agtccaagct tagcaaagac cccaacgaga
     5641 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5701 acgagctgta caagtaagcg gccgcggtaa caattgttaa ctaacttaag ctagcaacgg
     5761 tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc gaagccgctt
     5821 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5881 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5941 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     6001 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     6061 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     6121 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     6181 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     6241 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     6301 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atggtcatga
     6361 aaacatttaa catttctcaa caagatctag aattagtaga agtagcgaca gagaagatta
     6421 caatgcttta tgaggataat aaacatcatg tgggagcggc aattcgtacg aaaacaggag
     6481 aaatcatttc ggcagtacat attgaagcgt atataggacg agtaactgtt tgtgcagaag
     6541 ccattgcgat tggtagtgca gtttcgaatg gacaaaagga ttttgacacg attgtagctg
     6601 ttagacaccc ttattctgac gaagtagata gaagtattcg agtggtaagt ccttgtggta
     6661 tgtgtaggga gttgatttca gactatgcac cagattgttt tgtgttaata gaaatgaatg
     6721 gcaagttagt caaaactacg attgaagaac tcattccact caaatatacc cgaaattaaa
     6781 agtcgagatg catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag
     7081 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7261 gcggatctta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     7321 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     7381 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     7441 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     7501 ctagggttaa tctagtatac gcgtttaatt aatccagctt ttgttccctt tagtgagggt
     7561 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8401 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9721 cccgaaaagt gccac
//
