LOCUS       sequence-362311-       10478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OptoSTIM1(CRY2clust).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, 
            Terai K
  TITLE     Calcium transients trigger switch-like discharge of prostaglandin 
            E(2) in an extracellular signal-regulated kinase-dependent manner.
  JOURNAL   eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727.
  PUBMED    38276879
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-01-26"; volume: "12:RP86727. doi"; pages: " 
            10.7554/eLife.86727"
COMMENT     Sequence Label: pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)
FEATURES             Location/Qualifiers
     source          1..10478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1669..2685
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2609..2631
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2693..2712
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2752..2761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2758..2760,2761..2763,2764..3474)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(2803..2824)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5045..5062)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6537..7086
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6681..6698)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6908..6927
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7557..7574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7563..7787
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7834..7850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7858..7874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7882..7888,7889..7906,7907..7912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7927..7948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8329..8345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8353..8369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8377..8383,8384..8401,8402..8407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8422..8443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8731..9319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8811..8830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9490..10281,10282..10350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10113..10132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10351..10455)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gnsgcgcgcc rggsggggsg gggsggggsg rggggsgggg sggggsgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1741 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1801 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1861 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1921 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1981 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2041 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2101 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2161 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2221 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2281 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2341 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2401 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2461 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2521 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2581 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2641 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2701 ctggttattg tgctgtctca tcattttggc aaagaattgg gatccgaatt cgccaccatg
     2761 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2821 gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg agggcgatgc caccaacggc
     2881 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2941 gtgaccaccc tgagccacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     3001 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3061 aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3121 aaccgcatcg agctgaaggg cgttgacttc aaggaggacg gcaacatcct ggggcacaag
     3181 ctggagtaca acttcaacag ccacaacatc tatatcatgg ccgtcaagca gaagaacggc
     3241 atcaaggtga acttcaagat ccgccacaac gtggaggacg gcagcgtgca gctcgccgac
     3301 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga cagccactac
     3361 ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3421 ctggagttcc gcaccgccgc cgggatcact ctcggcatgg acgagctgta caagccaccg
     3481 gtcgccacca tgaagatgga caaaaagacc atcgtctggt ttcggagaga tttgagaata
     3541 gaagataatc ccgcgctcgc cgccgcggcc cacgagggtt ccgtcttccc cgttttcatt
     3601 tggtgtcctg aagaagaagg ccagttttat cccggaaggg cctctaggtg gtggatgaag
     3661 caaagtctgg cccatcttag ccagtcactg aaagcactgg gcagtgatct taccctgatc
     3721 aagacacaca ataccatctc tgccatcctc gactgcatca gggtgaccgg cgcaacgaaa
     3781 gtcgtgttta accacctgta cgatccagtt agtctggtgc gcgaccacac tgtgaaggag
     3841 aagctggtgg aacgggggat cagtgtgcag agctacaacg gggaccttct gtacgagcca
     3901 tgggagatct attgcgagaa agggaaaccg ttcacctcct tcaacagtta ctggaagaaa
     3961 tgtttggata tgtcaataga gtccgttatg ttgccccctc cctggagact gatgccgatt
     4021 actgctgctg cagaggccat ctgggcctgc tccatcgagg aactcggtct ggaaaatgaa
     4081 gcagaaaagc caagcaatgc acttctcact agagcctgga gccccggctg gtctaatgcc
     4141 gacaagctgc ttaacgagtt catcgaaaaa caactgattg actacgcgaa gaactccaag
     4201 aaagtggtag gtaactcaac tagcttgctc tctccatatc tccattttgg cgagatttct
     4261 gtccgccatg tatttcagtg cgctcggatg aaacagatta tctgggctcg cgataaaaac
     4321 agcgaaggcg aagaaagcgc cgatctgttc ctgcgaggga tcggacttcg ggaatactcc
     4381 cggtatatat gtttcaactt tccattcaca cacgagcaga gtctgttgtc ccacctcagg
     4441 ttcttcccct gggacgccga tgtcgacaag ttcaaagcct ggcggcaggg aagaacaggc
     4501 tatcccctgg tggatgctgg aatgagagag ctgtgggcca ctggctggat gcacaatagg
     4561 atccgcgtga ttgtgagctc ctttgctgtg aagttcctgc tgctgccttg gaagtggggc
     4621 atgaaatatt tttgggatac actgctggat gccgacctgg agtgtgacat cctggggtgg
     4681 cagtacatct ccggatctat tccagatggc catgaactgg ataggctgga caaccccgct
     4741 ctgcagggcg ctaagtatga ccctgaggga gaatacatca ggcagtggct gcctgagctg
     4801 gctaggctgc caactgaatg gattcaccat ccctgggacg ctcctctgac agtgctgaag
     4861 gccagtgggg tggagctggg aactaattac gctaaaccta tcgtggatat tgacaccgcc
     4921 agagagctgc tggctaaggc catctcccgg accagagaag cccagatcat gattggcgct
     4981 gccgctcgag atcctccaga cctcgataac gtgtacagtg ggggaggcgg agggggtggt
     5041 tcaggtggtg gtggtggtgg tggttcgggt ggtggcggag ggggaggctc cggactcaga
     5101 tctaaggagc acatgaagaa gatgatgaag gacttggagg ggttacaccg agctgagcag
     5161 agtctgcatg accttcagga aaggctgcac aaggcccagg aggagcaccg cacagtggag
     5221 gtggagaagg tccatctgga aaagaagctg cgcgatgaga tcaaccttgc taagcaggaa
     5281 gcccagcggc tgaaggagct gcgggagggt actgagaatg agcggagccg ccaaaaatat
     5341 gctgaggagg agttggagca ggttcgggag gccttgagga aagcagagaa ggagctagaa
     5401 tctcacagct catggtatgc tccagaggcc cttcagaagt ggctgcagct gacacatgag
     5461 gtggaggtgc aatattacaa catcaagaag caaaatgctg agaagcagct gctggtggcc
     5521 aaggaggggg ctgagaagat aaaaaagaag agaaacacac tctttggcac cttccacgtg
     5581 gcccacagct cttccctgga tgatgtagat cataaaattc taacagctaa gcaagcactg
     5641 agcgaggtga cagcagcatt gcgggagcgc ctgcaccgct ggcaacagat cgagatcctc
     5701 tgtggcttcc agattgtcaa caaccctggc atccactcac tggtggctgc cctcaacata
     5761 gaccccagct ggatgggcag tacacgcccc aaccctgctc acttcatcat gactgacgac
     5821 gtggatgaca tggatgagga gattgtgtct cccttgtcca tgcagtcccc tagcctgcag
     5881 agcagtgttc ggcagcgcct gacggagcca cagcatggcc tgggatctca gagagatttg
     5941 acccattccg attcggagtc ctccctccac atgagtgacc gccagcgtgt ggcccccaaa
     6001 cctcctcaga tgagccgtgc tgcagacgag gctctcaatg ccatgacttc caatggcagc
     6061 caccggctga tcgagggggt ccacccaggg tctctggtgg agaaactgcc tgacagccct
     6121 gccctggcca agaaggcatt actggcgctg aaccatgggc tggacaaggc ccacagcctg
     6181 atggagctga gcccctcagc cccacctggt ggctctccac atttggattc ttcccgttct
     6241 cacagcccca gctccccaga cccagacaca ccatctccag ttggggacag ccgagccctg
     6301 caagccagcc gaaacacacg cattccccac ctggctggca agaaggctgt ggctgaggag
     6361 gataatggct ctattggcga ggaaacagac tccagcccag gccggaagaa gtttcccctc
     6421 aaaatcttta agaagcctct taagaagtag gtcgacgggc cgcggtaaca attgttaact
     6481 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     6541 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6601 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     6661 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     6721 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     6781 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     6841 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     6901 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     6961 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7021 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7081 ataataccat ggtcatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag
     7141 tagcgacaga gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa
     7201 ttcgtacgaa aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag
     7261 taactgtttg tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt
     7321 ttgacacgat tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag
     7381 tggtaagtcc ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg
     7441 tgttaataga aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca
     7501 aatatacccg aaattaaaag tcgagatgca tcgatgatct agagctcgct gatcagcctc
     7561 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7621 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7681 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7741 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7801 aagaacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7861 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7921 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7981 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8041 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8161 taacgcagga aagracmkrt aaaacacatg cgtcaatttt acgcatgatt atctttaacg
     8221 tacgtcacaa tatgattatc tttcyarggk yarkcwwgyw krsgmkkykr wggrctctak
     8281 skcttctgag gcggaaagaa cctgcagccc aagcttggcg taatcatggt catagctgtt
     8341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8401 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8461 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8521 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8641 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8701 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8761 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8821 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8881 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8941 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9001 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9061 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9301 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9361 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9421 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9481 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9601 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10381 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10441 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
