LOCUS       sequence-362338-       10474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iRaf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, 
            Terai K
  TITLE     Calcium transients trigger switch-like discharge of prostaglandin 
            E(2) in an extracellular signal-regulated kinase-dependent manner.
  JOURNAL   eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727.
  PUBMED    38276879
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-01-26"; volume: "12:RP86727. doi"; pages: " 
            10.7554/eLife.86727"
COMMENT     Sequence Label: pPBpuro-miRFP703-eDHFR(69K6)-cRaf
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2775..2784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2781..3725
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    6380..6929
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6524..6541)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6751..6770
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6932..7531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6932..6951)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7428..7448
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7558..7575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7564..7788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7835..7851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7859..7875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7883..7889,7890..7907,7908..7913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7928..7949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8183..8245
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8286..8306)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8286..8304)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8325..8341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8349..8365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8373..8379,8380..8397,8398..8403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8418..8439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8556..8573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8727..9315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8807..8826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9486..10277,10278..10346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10109..10128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10347..10451)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs ggggsgrggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atcctcacgt caattgtagc
     2761 tagcgctacc ggtcgccacc atggtagcag gtcatgcctc tggcagcccc gcattcggga
     2821 ccgcctctca ttcgaattgc gaacatgaag agatccacct cgccggctcg atccagccgc
     2881 atggcgcgct tctggtcgtc agcgaacatg atcatcgcgt catccaggcc agcgccaacg
     2941 ccgcggaatt tctgaatctc ggaagcgtac tcggcgttcc gctcgccgag atcgacggcg
     3001 atctgttgat caagatcctg ccgcatctcg atcccaccgc cgaaggcatg ccggtcgcgg
     3061 tgcgctgccg gatcggcaat ccctctacgg agtactgcgg tctgatgcat cggcctccgg
     3121 aaggcgggct gatcatcgaa ctcgaacgtg ccggcccgtc gatcgatctg tcaggcacgc
     3181 tggcgccggc gctggagcgg atccgcacgg cgggttcact gcgcgcgctg tgcgatgaca
     3241 ccgtgctgct gtttcagcag tgcaccggct acgaccgggt gatggtgtat cgtttcgatg
     3301 agcaaggcca cggcctggta ttctccgagt gccatgtgcc tgggctcgaa tcctatttcg
     3361 gcaaccgcta tccgtcgtcg ctggtcccgc agatggcgcg gcagctgtac gtgcggcagc
     3421 gcgtccgcgt gctggtcgac gtcacctatc agccggtgcc gctggagccg cggctgtcgc
     3481 cgctgaccgg gcgcgatctc gacatgtcgg gctgcttcct gcgctcgatg tcgccgatcc
     3541 atctgcagtt cctgaaggac atgggcgtgc gcgccaccct ggcggtgtcg ctggtggtcg
     3601 gcggcaagct gtggggcctg gttgtctgtc accattatct gccgcgcttc atccgtttcg
     3661 agctgcgggc gatctgcaaa cggctcgccg aaaggatcgc gacgcggatc accgcgcttg
     3721 agagcctgta caagtctggg tccgcagcca gtagcgccgg aagtatgatc agtctgattg
     3781 cggcgttagc ggtagatcgc gttatcggca tggaaaacgc catgccgtgg aacctgcctg
     3841 ccgatctcgc ctggtttaaa cgcaacacct taaataaacc cgtgattatg ggccgccata
     3901 cctgggaatc aatcggtcgt ccgttgccag gacgcaaaaa tattatcctc agcagtcaac
     3961 cgggtacgga caaaaaaaag aaaaagaaag atcgcgtaac gtgggtgaag tcggtggatg
     4021 aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt gattggcggc ggtcgcgttt
     4081 atgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac gcagaagtgg
     4141 aaggcgacac ccatttcccg gattacgagc cggatgactg ggaatcggta ttcagcgaat
     4201 tccacgatgc tgatgcgcag aactctcaca gctattgctt tgagattctg gagcggcgga
     4261 gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtc
     4321 ctcgaagcct cgagatggag cacatacagg gagcttggaa gacgatcagc aatggttttg
     4381 gattcaaaga tgccgtgttt gatggctcca gctgcatctc tcctacaata gttcagcagt
     4441 ttggctatca gcgccgggca tcagatgatg gcaaactcac agatccttct aagacaagca
     4501 acactatccg tgttttcttg ccgaacaagc aaagaacagt ggtcaatgtg cgaaatggaa
     4561 tgagcttgca tgactgcctt atgaaagcac tcaaggtgag gggcctgcaa ccagagtgct
     4621 gtgcagtgtt cagacttctc cacgaacaca aaggtaaaaa agcacgctta gattggaata
     4681 ctgatgctgc gtctttgatt ggagaagaac ttcaagtaga tttcctggat catgttcccc
     4741 tcacaacaca caactttgct cggaagacgt tcctgaagct tgccttctgt gacatctgtc
     4801 agaaattcct gctcaatgga tttcgatgtc agacttgtgg ctacaaattt catgagcact
     4861 gtagcaccaa agtacctact atgtgtgtgg actggagtaa catcagacaa ctcttattgt
     4921 ttccaaattc cactattggt gatagtggag tcccagcact accttctttg actatgcgtc
     4981 gtatgcgaga gtctgtttcc aggatgcctg ttagttctca gcacagatat tctacacctc
     5041 acgccttcac ctttaacacc tccagtccct catctgaagg ttccctctcc cagaggcaga
     5101 ggtcgacatc cacacctaat gtccacatgg tcagcaccac cctgcctgtg gacagcagga
     5161 tgattgagga tgcaattcga agtcacagcg aatcagcctc accttcagcc ctgtccagta
     5221 gccccaacaa tctgagccca acaggctggt cacagccgaa aacccccgtg ccagcacaaa
     5281 gagagcgggc accagtatct gggacccagg agaaaaacaa aattaggcct cgtggacaga
     5341 gagattcaag ctattattgg gaaatagaag ccagtgaagt gatgctgtcc actcggattg
     5401 ggtcaggctc ttttggaact gtttataagg gtaaatggca cggagatgtt gcagtaaaga
     5461 tcctaaaggt tgtcgaccca accccagagc aattccaggc cttcaggaat gaggtggctg
     5521 ttctgcgcaa aacacggcat gtgaacattc tgcttttcat ggggtacatg acaaaggaca
     5581 acctggcaat tgtgacccag tggtgcgagg gcagcagcct ctacaaacac ctgcatgtcc
     5641 aggagaccaa gtttcagatg ttccagctaa ttgacattgc ccggcagacg gctcagggaa
     5701 tggactattt gcatgcaaag aacatcatcc atagagacat gaaatccaac aatatatttc
     5761 tccatgaagg cttaacagtg aaaattggag attttggttt ggcaacagta aagtcacgct
     5821 ggagtggttc tcagcaggtt gaacaaccta ctggctctgt cctctggatg gccccagagg
     5881 tgatccgaat gcaggataac aacccattca gtttccagtc ggatgtctac tcctatggca
     5941 tcgtattgta tgaactgatg acgggggagc ttccttattc tcacatcaac aaccgagatc
     6001 agatcatctt catggtgggc cgaggatatg cctccccaga tcttagtaag ctatataaga
     6061 actgccccaa agcaatgaag aggctggtag ctgactgtgt gaagaaagta aaggaagaga
     6121 ggcctctttt tccccagatc ctgtcttcca ttgagctgct ccaacactct ctaccgaaga
     6181 tcaaccggag cgcttccgag ccatccttgc atcgggcagc ccacactgag gatatcaatg
     6241 cttgcacgct gaccacgtcc ccgaggctgc ctgtcttctg cggccgctag tcgacggtac
     6301 cgcgggcccg ggataagtca actaacttaa gctagcaacg gtttccctct agcgggatca
     6361 attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     6421 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     6481 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     6541 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     6601 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     6661 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     6721 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     6781 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     6841 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     6901 gtggttttcc tttgaaaaac acgataatac catgaccgag tacaagccca cggtgcgcct
     6961 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7021 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7081 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7141 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7201 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7261 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7321 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7381 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7441 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7501 ctggtgcatg acccgcaagc ccggtgcctg atcgatgatc tagagctcgc tgatcagcct
     7561 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7621 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7681 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7741 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7801 aaagaacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7861 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7921 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7981 cgggaaacct gtcgtgccag cggatcttaa aagttttgtt actttataga agaaattttg
     8041 agtttttgtt tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta
     8101 ttagtatgta agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct
     8161 cgatatacag accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg
     8221 tcacaatatg attatctttc tagggttaat ctagtatacg cgtttaatta atccagcttt
     8281 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     8341 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8401 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8461 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10441 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
