LOCUS       sequence-362340-        9538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gq DREADD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, 
            Terai K
  TITLE     Calcium transients trigger switch-like discharge of prostaglandin 
            E(2) in an extracellular signal-regulated kinase-dependent manner.
  JOURNAL   eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727.
  PUBMED    38276879
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-01-26"; volume: "12:RP86727. doi"; pages: " 
            10.7554/eLife.86727"
COMMENT     Sequence Label: pPBpuro-hM3D-P2Av3-mCherryNLS
FEATURES             Location/Qualifiers
     source          1..9538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             4533..4589
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4744..4762)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5012..5031
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5307..5327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5328..5348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5439..5988
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5583..5600)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5810..5829
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5991..6590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5991..6010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6487..6507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6617..6634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6623..6847
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6894..6910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6918..6934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6942..6948,6949..6966,6967..6972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6987..7008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7389..7405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7413..7429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7437..7443,7444..7461,7462..7467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7482..7503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7791..8379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7871..7890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8550..9341,9342..9410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9173..9192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9411..9515)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs ggggsgrggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc accatgacct
     2761 tgcacaataa cagtacaacc tcgcctttgt ttccaaacat cagctcctcc tggatacaca
     2821 gcccctccga tgcagggctg cccccgggaa ccgtcactca tttcggcagc tacaatgttt
     2881 ctcgagcagc tggcaatttc tcctctccag acggtaccac cgatgaccct ctgggaggtc
     2941 ataccgtctg gcaagtggtc ttcatcgctt tcttaacggg catcctggcc ttggtgacca
     3001 tcatcggcaa catcctggta attgtgtcat ttaaggtcaa caagcagctg aagacggtca
     3061 acaactactt cctcttaagc ctggcctgtg ccgatctgat tatcggggtc atttcaatga
     3121 atctgtttac gacctacatc atcatgaatc gatgggcctt agggaacttg gcctgtgacc
     3181 tctggcttgc cattgactgc gtagccagca atgcctctgt tatgaatctt ctggtcatca
     3241 gctttgacag atacttttcc atcacgaggc cgctcacgta ccgagccaaa cgaacaacaa
     3301 agagagccgg tgtgatgatc ggtctggctt gggtcatctc ctttgtcctt tgggctcctg
     3361 ccatcttgtt ctggcaatac tttgttggaa agagaactgt gcctccggga gagtgcttca
     3421 ttcagttcct cagtgagccc accattactt ttggcacagc catcgctggt ttttatatgc
     3481 ctgtcaccat tatgactatt ttatactgga ggatctataa ggaaactgaa aagcgtacca
     3541 aagagcttgc tggcctgcaa gcctctggga cagaggcaga gacagaaaac tttgtccacc
     3601 ccacgggcag ttctcgaagc tgcagcagtt acgaacttca acagcaaagc atgaaacgct
     3661 ccaacaggag gaagtatggc cgctgccact tctggttcac aaccaagagc tggaaaccca
     3721 gctccgagca gatggaccaa gaccacagca gcagtgacag ttggaacaac aatgatgctg
     3781 ctgcctccct ggagaactcc gcctcctccg acgaggagga cattggctcc gagacgagag
     3841 ccatctactc catcgtgctc aagcttccgg gtcacagcac catcctcaac tccaccaagt
     3901 taccctcatc ggacaacctg caggtgcctg aggaggagct ggggatggtg gacttggaga
     3961 ggaaagccga caagctgcag gcccagaaga gcgtggacga tggaggcagt tttccaaaaa
     4021 gcttctccaa gcttcccatc cagctagagt cagccgtgga cacagctaag acttctgacg
     4081 tcaactcctc agtgggtaag agcacggcca ctctacctct gtccttcaag gaagccactc
     4141 tggccaagag gtttgctctg aagaccagaa gtcagatcac taagcggaaa aggatgtccc
     4201 tggtcaagga gaagaaagcg gcccagaccc tcagtgcgat cttgcttgcc ttcatcatca
     4261 cttggacccc atacaacatc atggttctgg tgaacacctt ttgtgacagc tgcataccca
     4321 aaaccttttg gaatctgggc tactggctgt gctacatcaa cagcaccgtg aaccccgtgt
     4381 gctatgctct gtgcaacaaa acattcagaa ccactttcaa gatgctgctg ctgtgccagt
     4441 gtgacaaaaa aaagaggcgc aagcagcagt accagcagag acagtcggtc atttttcaca
     4501 agcgcgcacc cgagcaggcc ttgggcagcg gcgcgacgaa ttttagtcta ctgaaacaag
     4561 cgggagacgt ggaggaaaac cctggacctg gcggccgcat ggtgagcaag ggcgaggagg
     4621 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     4681 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4741 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     4801 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     4861 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4921 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     4981 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     5041 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     5101 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     5161 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     5221 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     5281 ctctcggcat ggacgagctg tctagaccta agaagaagcg taaggtgcct aagaagaagc
     5341 gtaaggtgta aggatccgtc gacggccgct ctagagtcaa ctaacttaag ctagcaacgg
     5401 tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc gaagccgctt
     5461 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5521 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5581 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5641 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5701 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5761 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5821 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5881 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5941 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atgaccgagt
     6001 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     6061 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     6121 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     6181 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     6241 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     6301 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     6361 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     6421 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     6481 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     6541 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tcgatgatct
     6601 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6661 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6721 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6781 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6841 tctatggctt ctgaggcgga aagaacctgc agcccaagct tggcgtaatc atggtcatag
     6901 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6961 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7021 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7081 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7201 ttatccacag aatcagggga taacgcakrw amrraccgat aaaacacatg cgtcaatttt
     7261 acgcatgatt atctttarsg cagcaagggg gaggattggg aagacaatag caggcatgct
     7321 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagccc aagcttggcg
     7381 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7441 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7501 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7561 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7621 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7681 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8221 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8521 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8581 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8641 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8701 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8761 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8821 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8881 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8941 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9001 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9061 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9121 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9181 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9241 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9301 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9361 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9421 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9481 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
