LOCUS       sequence-362341-        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression fusion protein ALKBH5H204A-dCas9 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Liu XM, Zhou J, Mao Y, Ji Q, Qian SB
  TITLE     Programmable RNA N(6)-methyladenosine editing by CRISPR-Cas9 
            conjugates.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):865-871. doi: 
            10.1038/s41589-019-0327-1. Epub 2019 Aug 5.
  PUBMED    31383972
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0327-1"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "865-871"
COMMENT     Sequence Label: ALKBH5H204A-dCas9
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1508..4906,4907..4909,4910..5506,5507,5508..5510,
                     5511..5512,5513..5515,5516..5608)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1508..1531,1532..1534,1535..4021,4022..4024,
                     4025..5608)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5609..5629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5636..5653
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5676..5693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5682..5906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5977..5993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5990..6012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6001..6017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6025..6031,6032..6049,6050..6055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6070..6091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6208..6225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6379..6967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6459..6478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7138..7929,7930..7998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7761..7780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7999..8103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8182..8201)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8373..8752
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg gcggccgcca gcggctacac
      301 ggacctgcgt gagaagctca agtccatgac gtcccgggac aactataagg cgggcagccg
      361 ggaggccgcc gccgctgccg cagccgccgt agccgccgca gccgcagccg ccgctgccgc
      421 cgaaccttac cctgtgtccg gggccaagcg caagtatcag gaggactcgg accccgagcg
      481 cagcgactat gaggagcagc agctgcagaa ggaggaggag gcgcgcaagg tgaagagcgg
      541 catccgccag atgcgcctct tcagccagga cgagtgcgcc aagatcgagg cccgcattga
      601 cgaggtggtg tcccgcgctg agaagggcct gtacaacgag cacacggtgg accgggcccc
      661 actgcgcaac aagtacttct tcggcgaagg ctacacttac ggcgcccagc tgcagaagcg
      721 cgggcccggc caggagcgcc tctacccgcc gggcgacgtg gacgagatcc ccgagtgggt
      781 gcaccagctg gtgatccaaa agctggtgga gcaccgcgtc atccccgagg gcttcgtcaa
      841 cagcgccgtc atcaacgact accagcccgg cggctgcatc gtgtctgccg tggaccccat
      901 ccacatcttc gagcgcccca tcgtgtccgt gtccttcttt agcgactctg cgctgtgctt
      961 cggctgcaag ttccagttca agcctattcg ggtgtcggaa ccagtgcttt ccctgccggt
     1021 gcgcagggga agcgtgactg tgctcagtgg atatgctgct gatgaaatca ctcactgcat
     1081 acggcctcag gacatcaagg agcgccgagc agtcatcatc ctcaggaaga caagattaga
     1141 tgcaccccgg ttggaaacaa agtccctgag cagctccgtg ttaccaccca gctatgcttc
     1201 agatcgcctg tcaggaaaca acagggaccc tgctctgaaa cccaagcggt cccaccgcaa
     1261 ggcagaccct gatgctgccc acaggccacg gatcctggag atggacaagg aagagaaccg
     1321 gcgctcggtg ctgctgccca cacaccggcg gaggggtagc ttcagctctg agaactactg
     1381 gcgcaagtca tacgagtcct cagaggactg ctctgaggca gcaggcagcc ctgcccgaaa
     1441 ggtgaagatg cggcggcaca gcggcagcga gactcccggg acctcagagt ccgccacacc
     1501 cgaaagtgat aaaaagtatt ctattggttt agccatcggc actaattccg ttggatgggc
     1561 tgtcataacc gatgaataca aagtaccttc aaagaaattt aaggtgttgg ggaacacaga
     1621 ccgtcattcg attaaaaaga atcttatcgg tgccctccta ttcgatagtg gcgaaacggc
     1681 agaggcgact cgcctgaaac gaaccgctcg gagaaggtat acacgtcgca agaaccgaat
     1741 atgttactta caagaaattt ttagcaatga gatggccaaa gttgacgatt ctttctttca
     1801 ccgtttggaa gagtccttcc ttgtcgaaga ggacaagaaa catgaacggc accccatctt
     1861 tggaaacata gtagatgagg tggcatatca tgaaaagtac ccaacgattt atcacctcag
     1921 aaaaaagcta gttgactcaa ctgataaagc ggacctgagg ttaatctact tggctcttgc
     1981 ccatatgata aagttccgtg ggcactttct cattgagggt gatctaaatc cggacaactc
     2041 ggatgtcgac aaactgttca tccagttagt acaaacctat aatcagttgt ttgaagagaa
     2101 ccctataaat gcaagtggcg tggatgcgaa ggctattctt agcgcccgcc tctctaaatc
     2161 ccgacggcta gaaaacctga tcgcacaatt acccggagag aagaaaaatg ggttgttcgg
     2221 taaccttata gcgctctcac taggcctgac accaaatttt aagtcgaact tcgacttagc
     2281 tgaagatgcc aaattgcagc ttagtaagga cacgtacgat gacgatctcg acaatctact
     2341 ggcacaaatt ggagatcagt atgcggactt atttttggct gccaaaaacc ttagcgatgc
     2401 aatcctccta tctgacatac tgagagttaa tactgagatt accaaggcgc cgttatccgc
     2461 ttcaatgatc aaaaggtacg atgaacatca ccaagacttg acacttctca aggccctagt
     2521 ccgtcagcaa ctgcctgaga aatataagga aatattcttt gatcagtcga aaaacgggta
     2581 cgcaggttat attgacggcg gagcgagtca agaggaattc tacaagttta tcaaacccat
     2641 attagagaag atggatggga cggaagagtt gcttgtaaaa ctcaatcgcg aagatctact
     2701 gcgaaagcag cggactttcg acaacggtag cattccacat caaatccact taggcgaatt
     2761 gcatgctata cttagaaggc aggaggattt ttatccgttc ctcaaagaca atcgtgaaaa
     2821 gattgagaaa atcctaacct ttcgcatacc ttactatgtg ggacccctgg cccgagggaa
     2881 ctctcggttc gcatggatga caagaaagtc cgaagaaacg attactccat ggaattttga
     2941 ggaagttgtc gataaaggtg cgtcagctca atcgttcatc gagaggatga ccaactttga
     3001 caagaattta ccgaacgaaa aagtattgcc taagcacagt ttactttacg agtatttcac
     3061 agtgtacaat gaactcacga aagttaagta tgtcactgag ggcatgcgta aacccgcctt
     3121 tctaagcgga gaacagaaga aagcaatagt agatctgtta ttcaagacca accgcaaagt
     3181 gacagttaag caattgaaag aggactactt taagaaaatt gaatgcttcg attctgtcga
     3241 gatctccggg gtagaagatc gatttaatgc gtcacttggt acgtatcatg acctcctaaa
     3301 gataattaaa gataaggact tcctggataa cgaagagaat gaagatatct tagaagatat
     3361 agtgttgact cttaccctct ttgaagatcg ggaaatgatt gaggaaagac taaaaacata
     3421 cgctcacctg ttcgacgata aggttatgaa acagttaaag aggcgtcgct atacgggctg
     3481 gggacgattg tcgcggaaac ttatcaacgg gataagagac aagcaaagtg gtaaaactat
     3541 tctcgatttt ctaaagagcg acggcttcgc caataggaac tttatgcagc tgatccatga
     3601 tgactcttta accttcaaag aggatataca aaaggcacag gtttccggac aaggggactc
     3661 attgcacgaa catattgcga atcttgctgg ttcgccagcc atcaaaaagg gcatactcca
     3721 gacagtcaaa gtagtggatg agctagttaa ggtcatggga cgtcacaaac cggaaaacat
     3781 tgtaatcgag atggcacgcg aaaatcaaac gactcagaag gggcaaaaaa acagtcgaga
     3841 gcggatgaag agaatagaag agggtattaa agaactgggc agccagatct taaaggagca
     3901 tcctgtggaa aatacccaat tgcagaacga gaaactttac ctctattacc tacaaaatgg
     3961 aagggacatg tatgttgatc aggaactgga cataaaccgt ttatctgatt acgacgtcga
     4021 tgccattgta ccccaatcct ttttgaagga cgattcaatc gacaataaag tgcttacacg
     4081 ctcggataag aaccgaggga aaagtgacaa tgttccaagc gaggaagtcg taaagaaaat
     4141 gaagaactat tggcggcagc tcctaaatgc gaaactgata acgcaaagaa agttcgataa
     4201 cttaactaaa gctgagaggg gtggcttgtc tgaacttgac aaggccggat ttattaaacg
     4261 tcagctcgtg gaaacccgcc aaatcacaaa gcatgttgca cagatactag attcccgaat
     4321 gaatacgaaa tacgacgaga acgataagct gattcgggaa gtcaaagtaa tcactttaaa
     4381 gtcaaaattg gtgtcggact tcagaaagga ttttcaattc tataaagtta gggagataaa
     4441 taactaccac catgcgcacg acgcttatct taatgccgtc gtagggaccg cactcattaa
     4501 gaaatacccg aagctagaaa gtgagtttgt gtatggtgat tacaaagttt atgacgtccg
     4561 taagatgatc gcgaaaagcg aacaggagat aggcaaggct acagccaaat acttctttta
     4621 ttctaacatt atgaatttct ttaagacgga aatcactctg gcaaacggag agatacgcaa
     4681 acgaccttta attgaaacca atggggagac aggtgaaatc gtatgggata agggccggga
     4741 cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc aacatagtaa agaaaactga
     4801 ggtgcagacc ggagggtttt caaaggaatc gattcttcca aaaaggaata gtgataagct
     4861 catcgctcgt aaaaaggact gggacccgaa aaagtacggt ggcttcgata gccctacagt
     4921 tgcctattct gtcctagtag tggcaaaagt tgagaaggga aaatccaaga aactgaagtc
     4981 agtcaaagaa ttattgggga taacgattat ggagcgctcg tcttttgaaa agaaccccat
     5041 cgacttcctt gaggcgaaag gttacaagga agtaaaaaag gatctcataa ttaaactacc
     5101 aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg atgttggcta gcgccggaga
     5161 gcttcaaaag gggaacgaac tcgcactacc gtctaaatac gtgaatttcc tgtatttagc
     5221 gtcccattac gagaagttga aaggttcacc tgaagataac gaacagaagc aactttttgt
     5281 tgagcagcac aaacattatc tcgacgaaat catagagcaa atttcggaat tcagtaagag
     5341 agtcatccta gctgatgcca atctggacaa agtattaagc gcatacaaca agcacaggga
     5401 taaacccata cgtgagcagg cggaaaatat tatccatttg tttactctta ccaacctcgg
     5461 cgctccagcc gcattcaagt attttgacac aacgatagat cgcaaacgat acacttctac
     5521 caaggaggtg ctagacgcga cactgattca ccaatccatc acgggattat atgaaactcg
     5581 gatagatttg tcacagcttg ggggtgaccc caagaagaag aggaaagtca ccggtcatca
     5641 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5701 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5761 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5821 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5881 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat
     5941 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta
     6061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7021 acgttaaggg attttggtca tgagattatc aaaaagaatc ttcacctaga tccttttaaa
     7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8101 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga
     8161 tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     8221 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8281 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     8341 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     8401 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     8461 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     8521 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     8581 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     8641 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     8701 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tg
//
