LOCUS       sequence-362382-        8557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient transfection plasmid expressing 
            Renilla-luciferase-beta-globin fusion protein with PTC in 
            beta-globin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: pCMV-3XFLAG-renilla-luciferase-beta-globin-(39...
FEATURES             Location/Qualifiers
     source          1..8557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1015..1944
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="TSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    1972..1996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3258..3282)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3316..3938
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3515..3534)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3962..3982)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3980..4277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4331..5125
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4385..4404)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4995..5014
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5777..5911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5814..5833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5868..5887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5916..5935)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5917..5935)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5949..5965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5949..5965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5962..5984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5973..5989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5997..6003,6004..6021,6022..6027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6042..6063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6180..6197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6351..6939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6431..6450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7110..7901,7902..7970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7733..7752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7971..8075)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8102..8530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8189..8208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8399..8420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcaacttcg
     1021 aaagtttatg atccagaaca aaggaaacgg atgataactg gtccgcagtg gtgggccaga
     1081 tgtaaacaaa tgaatgttct tgattcattt attaattatt atgattcaga aaaacatgca
     1141 gaaaatgctg ttattttttt acatggtaac gcggcctctt cttatttatg gcgacatgtt
     1201 gtgccacata ttgagccagt agcgcggtgt attataccag accttattgg tatgggcaaa
     1261 tcaggcaaat ctggtaatgg ttcttatagg ttacttgatc attacaaata tcttactgca
     1321 tggtttgaac ttcttaattt accaaagaag atcatttttg tcggccatga ttggggtgct
     1381 tgtttggcat ttcattatag ctatgagcat caagataaga tcaaagcaat agttcacgct
     1441 gaaagtgtag tagatgtgat tgaatcatgg gatgaatggc ctgatattga agaagatatt
     1501 gcgttgatca aatctgaaga aggagaaaaa atggttttgg agaataactt cttcgtggaa
     1561 accatgttgc catcaaaaat catgagaaag ttagaaccag aagaatttgc agcatatctt
     1621 gaaccattca aagagaaagg tgaagttcgt cgtccaacat tatcatggcc tcgtgaaatc
     1681 ccgttagtaa aaggtggtaa acctgacgtt gtacaaattg ttaggaatta taatgcttat
     1741 ctacgtgcaa gtgatgattt accaaaaatg tttattgaat cggacccagg attcttttcc
     1801 aatgctattg ttgaaggtgc caagaagttt cctaatactg aatttgtcaa agtaaaaggt
     1861 cttcattttt cgcaagaaga tgcacctgat gaaatgggaa aatatatcaa atcgttcgtt
     1921 gagcgagttc tcaaaaatga acaagcgaat tcatcgatag atctgatatc aacaagtttg
     1981 tacaaaaaag caggctccgc ggccgccccc ttcaccgtgc atctgactcc tgaggagaag
     2041 tctgccgtta ctgccctgtg gggcaaggtg aacgtggatg aagttggtgg tgaggccctg
     2101 ggcaggttgg tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcatgt
     2161 ggagacagag aagactcttg ggtttctgat aggcactgac tctctctgcc tattggtcta
     2221 ttttcccacc cttaggctgc tggtggtcta cccttggacc tagaggttct ttgagtcctt
     2281 tggggatctg tccactcctg atgctgttat gggcaaccct aaggtgaagg ctcatggcaa
     2341 gaaagtgctc ggtgccttta gtgatggcct ggctcacctg gacaacctca agggcacctt
     2401 tgccacactg agtgagctgc actgtgacaa gctgcacgtg gatcctgaga acttcagggt
     2461 gagtctgatg ggcacctcct gggtttcctt cccctggcta ttctgctcaa ccttcctatc
     2521 agaaaaaaag gggaagcgat tctagggagc agtctccatg actgtgtgtg gagtgttgac
     2581 aagagttcgg atattttatt ctctactcag aattgctgct ccccctcact ctgttctgtg
     2641 ttgtcatttc ctctttcttt ggtaagcttt taatttccag ttgcatttta ctaaattaat
     2701 taagctggtt atttacttcc catcctgata tcagcttccc ctcctccttt cctcccagtc
     2761 cttctctctc tcctctctct ttctctaatc ctttcctttc cctcagttca tttcttcttc
     2821 tttgatctac gtttgtttgt ctttttaaat attgccttgt aacttgctca gaggacaagg
     2881 aagatatgtc cctgtttctt ctcatagctc tcaagaatag tagcataatt ggcttttatg
     2941 ccagggtgac aggggaagaa tatattttac atataaattc tgtttgacat aggattctta
     3001 taataatttg tcagtagttt aaggttgcaa acaaatgtct ttgtaaataa gcctgcagta
     3061 tctggtattt ttgctctaca gttatgttga tggttcttcc atattcccac agctcctggg
     3121 caatatgatc gtgattgtgc tgggccacca cctgggcaag gatttcaccc ccgctgcaca
     3181 ggctgccttc cagaaggtgg tggctggagt ggccactgcc ctggcccaca agtatcacta
     3241 aaagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgatatcg gtaccagtcg
     3301 actctagagg atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3361 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3421 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     3481 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     3541 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     3601 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     3661 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     3721 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3781 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     3841 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     3901 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg
     3961 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4021 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4141 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4201 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4261 tgaggaggct tttttggagg aattgatcag cttgggatct gatcaagaga caggatgagg
     4321 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4381 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4441 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4501 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4561 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4621 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4681 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4741 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4801 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4861 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4921 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4981 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5041 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5101 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5161 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5221 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5281 gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg
     5341 ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc
     5401 agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct
     5461 ttggtcgacc cggacgggac gctcctgcgc ctgatacaga acgaattgct tgcaggcatc
     5521 tcatgagtgt gtcttcccgt tttccgcctg aggtcactgc gtggatggag cgctggcgcc
     5581 tgctgcgcga cggcgagctg ctcaccaccc actcgccaag ctggaaccgt aaaaaggccg
     5641 cgttgctggc gtttttccat aggctccgcc gatcataatc agccatacca catttgtaga
     5701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5881 actcatcaat gtatcttatc atgtctggat caattcccta tagtgagtcg tattaaattc
     5941 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6001 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6061 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6121 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6181 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6241 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6301 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6361 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6421 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6481 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6541 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6601 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6661 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6721 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6781 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6841 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6901 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6961 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7021 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7081 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7141 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7201 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7261 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7321 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7381 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7441 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7501 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7561 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7621 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7681 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7741 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7801 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7861 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7921 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7981 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8041 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8101 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8161 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8221 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8281 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8341 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8401 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8461 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8521 aacgcgaatt ttaacaaaat attaacgctt acaattt
//
