LOCUS       sequence-362472-        9214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p53 - Apoptosis gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE21
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2046..2077
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2104..2113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2110..3759
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3763..3882
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3974..4562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4027..4047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4445..4456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4577..4893
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4744..4879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4944..4946,4947..4949,4950..5663)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4989..5010)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5250..5269)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5597..5618
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5735..5968
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6040..6161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6077..6096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6131..6150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6201..6336
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6356..6375)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6357..6375)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6385..6402)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6385..6401)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6394..6416)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6543..6998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6630..6649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6840..6861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7024..7128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7129..7197,7198..7989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7347..7366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8160..8748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8649..8668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8902..8919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9036..9057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9072..9077,9078..9095,9096..9102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9110..9126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9115..9137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9134..9150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9134..9150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9169..9189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9171..9189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctacag aacatgtcta agcatgctgt gccttgcctg
     1981 gacttgcctg gccttgcctt ggggatccga gaacagatct ggcctcggcg gccaagctta
     2041 gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt
     2101 aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc
     2161 gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc
     2221 ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc
     2281 gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg
     2341 atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc
     2401 atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc
     2461 atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc
     2521 aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac
     2581 taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac
     2641 gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac
     2701 agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc
     2761 cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc
     2821 ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc
     2881 tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg
     2941 caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag
     3001 agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg
     3061 ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc
     3121 cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac
     3181 aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac
     3241 accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc
     3301 atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg
     3361 ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg
     3421 ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc
     3481 ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc
     3541 ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag
     3601 atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg
     3661 ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag
     3721 attctcatta aggccaagaa gggcggcaag atcgccgtga attctcacgg cttccctccc
     3781 gaggtggagg agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg
     3841 gatagacacc ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg cgactctaga
     3901 acgcgtcact gcggcttata tgcagaaagc gaagactaca gacatcagcg cgtaccggtt
     3961 agtaatgatc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4021 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4081 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4141 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4201 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4261 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4321 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     4381 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4441 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4501 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4561 gcctggcgat ggtaccggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4621 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4681 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4741 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4801 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4861 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4921 ttcggatctg atcagcacct agcatggtga gcaagggcga ggagctgttc accggggtgg
     4981 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     5041 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     5101 agctgcccgt gccctggccc accctcgtga ccaccttcac ctacggcgtg cagtgcttcg
     5161 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     5221 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     5281 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     5341 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata
     5401 tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg
     5461 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     5521 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     5581 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     5641 gcatggacga gctgtacaag taaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5701 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5761 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5821 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5881 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5941 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6001 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     6061 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6121 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6181 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6241 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6301 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6361 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6421 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6481 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6541 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6601 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6721 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6841 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6901 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6961 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     7021 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7081 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7141 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7201 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7261 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7321 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7381 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7441 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7501 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7561 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7621 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7681 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7741 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7801 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7861 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7921 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7981 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8041 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8101 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8161 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8221 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8281 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8341 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8401 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8461 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8521 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8581 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8641 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8701 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8761 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8821 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8881 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8941 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9001 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     9061 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9121 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     9181 cactaaaggg aacaaaagct ggagctgcaa gctt
//
