LOCUS       sequence-36248-e        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Taniguchi N, Carames B, Kawakami Y, Amendt BA, Komiya S, Lotz M
  TITLE     Chromatin protein HMGB2 regulates articular cartilage surface 
            maintenance via beta-catenin pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 29. 106(39):16817-22.
  PUBMED    19805379
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 29. 106(39):16817-22."
COMMENT     Sequence Label: pcDNA3.1 Flag mHMGB2
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             288..311
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(972..990)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(973..990)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgngggcttt agcgactctc tggctactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tccacaattc gggcggcggc tgctgctgcc
      121 tgtggcccgg gcggctggga gaagccgagt gttggtgagt gacgcggcgg aggtgtagtt
      181 tgacgcggtg tgttacgtgg gggagagaat aaaactccag cgagatccgg gccgcgaacg
      241 aaagcagtga cggaggagct tgtaccaccg gtgactagat gaccatggac tacaaggacg
      301 acgatgacaa gctgcaggaa ttcatgggca agggtgaccc caacaagccg cggggcaaaa
      361 tgtcctcgta cgccttcttc gtgcagacct gccgcgagga gcacaagaag aagcatcccg
      421 actcgtcggt gaacttcgcc gagttctcca agaaatgctc cgagagatgg aagaccatgt
      481 ctgcaaagga aaagtccaag tttgaagatt tggccaagag cgacaaagct cgttatgaca
      541 gggagatgaa gaactatgtt cctcccaaag gggataagaa aggaaagaaa aaagacccca
      601 atgctccgaa gagaccaccg tctgccttct tcctgttttg ctctgaaaat cgcccaaaga
      661 tcaaaattga acacccaggc ctgtctattg gagatactgc gaagaaactg ggtgagatgt
      721 ggtctgagca atctgccaaa gataaacaac cgtatgagca gaaagcagct aaactaaagg
      781 agaagtatga aaaggatatt gctgcatacc gtgccaaggg caaaagtgaa gcaggaaaga
      841 agggtcctgg taggccaaca ggctcaaaga agaagaacga accagaagat gaggaggagg
      901 aagaagagga ggaagaggag gaagatgaag aggaagaaga ggaggatgaa gaataatcta
      961 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatca
//
