LOCUS       sequence-362485-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Duan Y, Ma G, Huang X, D'Amore PA, Zhang F, Lei H
  TITLE     The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced 
            expression of MDM2 and proliferation and survival of cells.
  JOURNAL   J Biol Chem. 2016 May 31. pii: jbc.M116.729467.
  PUBMED    27246850
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 May 31. pii: jbc.M116.729467."
COMMENT     Sequence Label: pAAV-U6-sgRNA-CMV-GFP
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        375..678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        680..879
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     833..853
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aaacaccgga agagcgagct cttctgtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct agagggtacc
      301 ggggcccggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      361 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      421 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      481 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      541 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      601 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      661 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      721 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      781 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      841 ggcggtaggc gtgtacggtg ggaggtctat ataagcaga
//
