LOCUS       sequence-36249-e        6746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: pwB2b
FEATURES             Location/Qualifiers
     source          1..6746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..73)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        71..368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      238..372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1481..1602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1492..1511)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1546..1565
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1784..2962
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2015..2953
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2910..2930
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2986..3010
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3017..3026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4119..4143)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4205..4793
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4258..4278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4676..4687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4835..5407
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      5578..6166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6067..6086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6252..6299
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6291..6309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcaggg cctgaaataa cctctgaaag aggaacttgg ttaggtacct gtggaatgtg
       61 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
      181 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
      241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      301 atttatacag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      361 ttttttggag gcggccgcct aggcttttgc ataaatgggc gcagctctgg cattgctcgg
      421 agatctggtc gcatctgtgt ccgaagcagc cgctgcaact ggattcagcg tcgccgagat
      481 cgctgccggc gaagcagctg ccgcaatcga ggtccagatc gcaagtctgg ccactgtgga
      541 agggatcacc tctacaagcg aagccatcgc tgccatcgga ttgacccctc aaacctacgc
      601 cgtgatcgct ggcgcacctg gcgcaatcgc tggtttcgcc gcactgatcc agactgtcac
      661 cggaatctcg agcctcgccc aggtcggata tcggttcttc tcggactggg accataaggt
      721 cagtaccgtg gggttgtacc aacaaagcgg gatggccctc gagctcttca atcccgacga
      781 atactacgac atcctgttcc ctggcgtgaa caccttcgtg aacaacatcc agtatctgga
      841 cccccgccac tggggaccaa gcctgttcgc cacaatcagt caagccctct ggcacgtcat
      901 ccgcgacgac atccccgcca tcacaagcca ggagctccag cgccgcaccg aaaggttttt
      961 ccgcgatagt ctcgcaagat tcctcgagga gaccacatgg accatcgtga acgcacccgt
     1021 gaatttctac aactacatcc aagactacta ctccaacctg agtcccatcc gcccaagcat
     1081 ggtccgccaa gtcgccgagc gcgagggcac acaagtgaac ttcgggcaca catatcgcat
     1141 cgacgacgcc gattccatcc aggaggtcac acagcgcatg gaactgcgca acaaggaaaa
     1201 cgtgcacagc ggcgaattca tcgagaagac catcgcaccc ggcggcgcca accagcgcac
     1261 cgccccacaa tggatgctcc cactcttgct ggggctctat ggcaccgtga cccctgccct
     1321 ggaggcctac gaggacgggc caaatcagaa aaagcgccgc gtcagtcgcg ggtcaagtca
     1381 gaaggcaaag ggcacaaggg cctccgcaaa gaccaccaac aagcgccgct cccggtcgag
     1441 ccgctcatga gtgctagcgg gccgcttcga gcagacatga taagatacat tgatgagttt
     1501 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1561 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1621 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     1681 tacaaatgtg gtaaaatcga taaggatccg ggctggcgta atagcgaaga ggcccgcacc
     1741 gatcgccctt cccaacagtt gcggtggaga agagcatgcg tgaggctccg gtgcccgtca
     1801 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1861 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     1921 ccgccctttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ctgtgaacgt
     1981 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2041 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2101 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2161 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2221 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2281 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2341 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2401 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2461 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2521 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2581 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2641 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2701 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2761 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2821 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     2881 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     2941 tcttccattt caggtgtcgt gaggaattct ctagagcttg atcaaacaag tttgtacaaa
     3001 aaagcaggct tccggagcca ccatggcacc cacaaagcgc aagggcgaat gccccggcgc
     3061 cgccccaaag aaacccaagg agccagtcca ggtccccaag ttgctgatca agggcggcgt
     3121 ggaggtcctg gaggtcaaga ccggcgtgga cgccatcacc gaggtggagt gtttcttgaa
     3181 ccccgagatg ggcgaccccg acaacgatct gcgcgggtac agcttgcgcc tgaccgccga
     3241 gaccgcattc gattccgact cacccgatcg caagatgttg ccatgctatt ccaccgcccg
     3301 catcccactg cccaacctca acgaagactt gacatgcggc aacctgctga tgtgggaagc
     3361 cgtcaccgtg aagaccgaag tcatcggcat cacctccatg ttgaatctgc acgccggaag
     3421 ccaaaaggtg cacgagaacg gcggcgggaa gcccatccag gggtcaaact tccatttctt
     3481 cgccgtcggc ggcgatccac tcgagatgca aggcgtgttg atgaactatc gcaccaagta
     3541 tcccgagggc accgtgacac ccaagaaccc aaccgcccaa agtcaggtca tgaacaccga
     3601 tcacaaagca tatctcgata agaataacgc ctaccccgtc gagtgttgga tccccgatcc
     3661 atccaagaac gagaacaccc gctacttcgg cacctatacc ggcggcgaga acgtcccacc
     3721 cgtgttgcac gtgacaaata ccgccacaac cgtcctgctc gacgagcaag gcgtcggccc
     3781 actctgcaag gcagacagcc tctacgtcag cgccgccgac atctgcggac tgttcaccaa
     3841 ttcaagcggc acccagcaat ggcgcgggtt gccccgctac ttcaaaatca ggctccgcaa
     3901 gcgcagcgtg aagaatccct atccaatcag tttcctgctg tccgatttga tcaatcgccg
     3961 cacacaacgc gtcgacggcc agcccatgta cggcatggaa agccaagtcg aagaagtgcg
     4021 cgtcttcgac gggaccgagc agttgcccgg cgatcccgac atgatccgct acatcgatcg
     4081 ccaaggccag ctccaaacca agatggtctg agactagtac ccagctttct tgtacaaagt
     4141 ggttcgatct agaatggcta gtggatcccc cgggctgcag gaattcgata tcaagcttat
     4201 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4261 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4321 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4381 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4441 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4501 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4561 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4621 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4681 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4741 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     4801 ccgtcggccc actgctccct aaacctgagc tagcattatc cctaatacct gccaccccac
     4861 tcttaatcag tggtggaaga acggtctcag aactgtttgt ttcaattggc catttaagtt
     4921 tagtagtaaa agactggtta atgataacaa tgcatcgtaa aaccttcaga aggaaaggag
     4981 aatgttttgt ggaccacttt ggttttcttt tttgcgtgtg gcagttttaa gttattagtt
     5041 tttaaaatca gtacttttta atggaaacaa cttgaccaaa aatttgtcac agaattttga
     5101 gacccattaa aaaagttaaa tgagaaacct gtgtgttcct ttggtcaaca ccgagacatt
     5161 taggtgaaag acatctaatt ctggttttac gaatctggaa acttcttgaa aatgtaattc
     5221 ttgagttaac acttctgggt ggagaatagg gttgttttcc ccccacataa ttggaagggg
     5281 aaggaatatc atttaaagct atgggagggt ttctttgatt acaacactgg agagaaatgc
     5341 agcatgttgc tgattgcctg tcactaaaac aggccaaaaa ctgagtcctt gggttgcata
     5401 gaaagcttca tgttgctaaa ccaatgttaa gtgaatcttt ggaaacaaaa tgtttccaaa
     5461 ttactgggat gtgcatgttg aaacgtgggt taattaacta gccatgacca aaatccctta
     5521 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5641 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5701 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5761 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5821 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5881 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5941 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6001 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6061 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6121 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6181 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct taattaaatt
     6241 tttcaaaagt agttgacaat taatcatcgg catagtatat cggcatagta taatacgact
     6301 cactatagga gggccatcat ggccaagttg accagtgctg tcccagtgct cacagccagg
     6361 gatgtggctg gagctgttga gttctggact gacaggttgg ggttctccag agattttgtg
     6421 gaggatgact ttgcaggtgt ggtcagagat gatgtcaccc tgttcatctc agcagtccag
     6481 gaccaggtgg tgcctgacaa caccctggct tgggtgtggg tgagaggact ggatgagctg
     6541 tatgctgagt ggagtgaggt ggtctccacc aacttcaggg atgccagtgg ccctgccatg
     6601 acagagattg gagagcagcc ctgggggaga gagtttgccc tgagagaccc agcaggcaac
     6661 tgtgtgcact ttgtggcaga ggagcaggac tgaggataag aattgtaaca aaaaaccccg
     6721 ccccggcggg gttttttgtt aattaa
//
