LOCUS       sequence-36250-e        5274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: pMslc
FEATURES             Location/Qualifiers
     source          1..5274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          449..1396
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     misc_feature    3310..3754
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        3788..3834
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3826..3844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4306..4427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4317..4336)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4371..4390
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4615..5203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5104..5123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggctga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagttc cgccagagcg cgcgagggcc tccagcggcc gcccctcccc cacagcaggg
      301 gcggggtccc gcgcccaccg gaaggagcgg gctcggggcg ggcggcgctg attggccggg
      361 gcgggcctga cgccgacgcg gctataagag accacaagcg acccgcaggg ccagacgttc
      421 ttcgccgaag cttgccgtca gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc
      481 gagctggagg tcctgctccg agcgggccgg gccccgctgt cgtcggcggg gattagctgc
      541 gagcattccc gcttcgagtt gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa
      601 ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc
      661 gtgctactcc ggccgcactc tggtcttttt tttttttgtt gttgttgccc tgctgccttc
      721 gattgccgtt cagcaatagg ggctaacaaa gggagggtgc ggggcttgct cgcccggagc
      781 ccggagaggt catggttggg gaggaatgga gggacaggag tggcggctgg ggcccgcccg
      841 ccttcggagc acatgtccga cgccacctgg atggggcgag gcctggggtt tttcccgaag
      901 caaccaggct ggggttagcg tgccgaggcc atgtggcccc agcacccggc acgatctggc
      961 ttggcggcgc cgcgttgccc tgcctcccta actagggtga ggccatcccg tccggcacca
     1021 gttgcgtgcg tggaaagatg gccgctcccg ggccctgttg caaggagctc aaaatggagg
     1081 acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga gggcctggtc
     1141 cctcaccggc tgctgcttcc tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg
     1201 gcttttgagc acggctagtc gcggcggggg gaggggatgt aatggcgttg gagtttgttc
     1261 acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt tttggaattt
     1321 gtccccttga gttttgagcg gagctaattc tcgggcttct tagcggttca aaggtatctt
     1381 ttaaaccctt ttttaggtgt tgtgaaaacc accgctaatt caaagcaacc ggtgatatcg
     1441 gatccacgcg taccatggct gccccgagag acaatgtcac tttattattc aagttatact
     1501 gcttggcagt gatgaccctg atggctgcag tctataccat agctttaaga tacacaagga
     1561 catcagacaa agaactctac ttttcaacca cagccgtgtg tatcacagaa gttataaagt
     1621 tattgctaag tgtgggaatt ttagctaaag aaactggtag tctgggtaga ttcaaagcat
     1681 ctttaagaga aaatgtcttg gggagcccca aggaactgtt gaagttaagt gtgccatcgt
     1741 tagtgtatgc tgttcagaac aacatggctt tcctagctct tagcaatctg gatgcagcag
     1801 tgtaccaggt gacctaccag ttgaagattc cgtgtactgc tttatgcact gttttaatgt
     1861 taaaccggac actcagcaaa ttacagtggg tttcagtttt tatgctgtgt gctggagtta
     1921 cgcttgtaca gtggaaacca gcccaagcta caaaagtggt ggtggaacaa aatccattat
     1981 tagggtttgg cgctatagct attgctgtat tgtgctcagg atttgcagga gtatattttg
     2041 aaaaagtttt aaagagttca gatacttctc tttgggtgag aaacattcaa atgtatctat
     2101 cagggattat tgtgacatta gctggcgtct acttgtcaga tggagctgaa attaaagaaa
     2161 aaggattttt ctatggttac acatattatg tctggtttgt catctttctt gcaagtgttg
     2221 gtggcctcta cacttctgtt gtggttaagt acacagacaa catcatgaaa ggcttttctg
     2281 cagcagcggc cattgtcctt tccaccattg cttcagtaat gctgtttgga ttacagataa
     2341 cactcacctt tgccctgggt actcttcttg tatgtgtttc catatatctc tatggattac
     2401 ccagacaaga cactacatcc atccaacaag gagaaacagc ttcaaaggag agagttattg
     2461 gtgtgtgatt ttagcctcac gtgagactcc ttttaagact aaaccatttg cattaaacta
     2521 gagccttaag tcaatctcag aaggtagcat aaacaaataa aaattaactg tatggcatga
     2581 tcagtgcggt tatgtggaaa caacaacaaa caaacgaagc tatctgagtg aactgctaat
     2641 acagaaactt aatgtagacc tgtttggggt ctactattgt tttagaatga aggaattgta
     2701 ttattgtgtg tatatataat ttgtaaataa aaagtatgga gatgatacgg tgttaaaaaa
     2761 aatcatggta aggctacaat actcaagtaa caaggtttgg gacaatgtct aagggttaaa
     2821 gtgccaaagc catttctgta ctaactgttc tcttgttccg gtaccgggga gaaggatgac
     2881 ccctccttat tctccaattc atgtacagta ttttgtccta gcagcataaa gacctagctc
     2941 ttttcttaca agaggcagaa acaagacagg ctagttcata aacaaactgt gtaacttctc
     3001 aaaatgaatc tatttcataa ctcggacaat ttctgggtgg tgactgagta cccctttagt
     3061 gagtacccct ttagtgctat atttgtgcca ttcattatct ggttcatatt tcttttctgt
     3121 tagatgatac acatttcttc aaaaaaattt ctaatgtcac ttttgtactt ttttaaataa
     3181 agtatgttta actgttgggc tctcaataat ttgtgaaatt tcagtgtttt ctataatgtt
     3241 aatggggaaa ttcagcaata aactttattt gtaagaaaga aaaaaaaaaa aaaaaaactc
     3301 gactctagga gcaggtttcc ccaatgacac aaaacgtgca acttgaaact ccgcctggtc
     3361 tttccaggtc tagaggggta acactttgta ctgcgtttgg ctccacgctc gatccactgg
     3421 cgagtgttag taacagcact gttgcttcgt agcggagcat gacggccgtg ggaactcctc
     3481 cttggtaaca aggacccacg gggccaaaag ccacgcccac acgggcccgt catgtgtgca
     3541 accccagcac ggcgacttta ctgcgaaacc cactttaaag tgacattgaa actggtaccc
     3601 acacactggt gacaggctaa ggatgccctt caggtacccc gaggtaacac gcgacactcg
     3661 ggatctgaga aggggactgg ggcttctata aaagcgctcg gtttaaaaag cttctatgcc
     3721 tgaataggtg accggaggtc ggcacctttc ctttgcaatt actgacccta tgaatacaac
     3781 tgactgtttg acaattaatc atcggcatag tatatcggca tagtataata cgactcacta
     3841 taggagggcc accatgaaga ccttcaacat ctctcagcag gatctggagc tggtggaggt
     3901 cgccactgag aagatcacca tgctctatga ggacaacaag caccatgtcg gggcggccat
     3961 caggaccaag actggggaga tcatctctgc tgtccacatt gaggcctaca ttggcagggt
     4021 cactgtctgt gctgaagcca ttgccattgg gtctgctgtg agcaacgggc agaaggactt
     4081 tgacaccatt gtggctgtca ggcaccccta ctctgatgag gtggacagat ccatcagggt
     4141 ggtcagcccc tgtggcatgt gcagagagct catctctgac tatgctcctg actgctttgt
     4201 gctcattgag atgaatggca agctggtcaa aaccaccatt gaggaactca tccccctcaa
     4261 gtacaccagg aactaaacct gaattcgcta gctggccaga catgataaga tacattgatg
     4321 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4381 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4441 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4501 acctctacaa atgtggtatg gaaatgttaa ttaactagcc atgaccaaaa tcccttaacg
     4561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4741 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5221 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttcttaa ttaa
//
