LOCUS       sequence-362511-        9445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The Tet3G gene is expressed under a constitutive EF1-alpha promoter.
            This protein binds a TRE3G promoter to activate gene transcription 
            only in the presence of tetracycline or its analogs (e.g. 
            doxycycline).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Domenig SA, Bundschuh N, Lenardic A, Ghosh A, Kim I, Qabrati X, 
            D'Hulst G, Bar-Nur O
  TITLE     CRISPR/Cas9 editing of directly reprogrammed myogenic progenitors 
            restores dystrophin expression in a mouse model of muscular 
            dystrophy.
  JOURNAL   Stem Cell Reports. 2022 Feb 8;17(2):321-336. doi: 
            10.1016/j.stemcr.2021.12.003. Epub 2022 Jan 6.
  PUBMED    34995499
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.12.003"; journalName: "Stem Cell Reports"; 
            date: "2022-02-8- 8"; volume: "17"; issue: "2"; pages: "321-336"
COMMENT     Sequence Label: pLV[Exp]-Neo-EF1A>Tet3G
FEATURES             Location/Qualifiers
     source          1..9445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..237
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             238..418
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    465..590
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1083..1316
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1501..1545
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1811..1928
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1946..1966
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1967..3145
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2198..3136
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3093..3113
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3145..3169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3176..3922
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(3923..3947)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3965..4553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4018..4038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4436..4447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4584..5083
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4619..4642)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5016..5035
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5097..5891
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5097..5891
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5151..5170)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5761..5780
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5974..6207
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6279..6400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6316..6335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6370..6389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6440..6575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6595..6614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6596..6614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6624..6641)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6624..6640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6633..6655)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6782..7237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6869..6888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7079..7100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7263..7367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7368..7436,7437..8228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7586..7605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8399..8987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8888..8907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9141..9158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9275..9296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9311..9316,9317..9334,9335..9341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9349..9365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9354..9376
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9373..9389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9373..9389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9408..9428
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9410..9428
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
       61 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      121 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      181 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      421 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      481 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      541 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      601 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      661 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      721 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      781 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      841 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      901 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      961 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1021 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1081 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1141 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1201 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1261 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1321 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1381 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1441 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1501 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1561 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1621 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1681 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     1741 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     1801 tatcgctagc ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     1861 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     1921 aaaattttac tagtgattat cggatcaact ttgtatagaa aagttgggct ccggtgcccg
     1981 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2041 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2101 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2161 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2221 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2281 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2341 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2401 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2461 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2521 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2581 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2641 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc
     2701 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2761 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     2821 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     2881 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     2941 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3001 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3061 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3121 ttttcttcca tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatgtc
     3181 tagactggac aagagcaaag tcataaactc tgctctggaa ttactcaatg gagtcggtat
     3241 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     3301 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     3361 gcatcatacc cactcctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     3421 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     3481 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     3541 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     3601 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     3661 caccgattct atgcccccac ttctgaaaca agcaattgag ctgttcgacc ggcagggagc
     3721 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     3781 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc
     3841 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     3901 ccttgacatg ctccccgggt aaacccagct ttcttgtaca aagtggtgat aatcgaattc
     3961 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4021 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4081 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4141 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4201 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4261 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4321 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4381 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4441 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4501 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga
     4561 attcccgcgg ttcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     4621 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     4681 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     4741 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     4801 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     4861 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     4921 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     4981 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     5041 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcac gtgcgcatga
     5101 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5161 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5221 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     5281 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5341 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5401 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5461 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5521 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5581 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     5641 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5701 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5761 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5821 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5881 agttcttctg agcgggactc tgggtacctt taagaccaat gacttacaag gcagctgtag
     5941 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6001 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6061 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6181 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6241 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6361 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6421 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6481 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6541 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6601 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6661 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6721 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6781 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7081 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7141 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7201 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7261 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7321 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7381 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7441 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7501 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7561 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7621 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7681 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7741 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7801 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7861 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7921 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7981 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8041 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8101 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8161 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8221 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8281 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8341 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8401 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8461 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8521 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8581 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8641 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8701 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8761 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga
     8821 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8881 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8941 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9001 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9061 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9181 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9361 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     9421 actaaaggga acaaaagctg gagct
//
