LOCUS       sequence-362512-       10266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal expression (Cre-off) of an archaerhodopsin-derived 
            genetically encoded voltage indicator QuasAr6b carrying a Citrine 
            expression tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum 
            S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot 
            V, Deisseroth K, Cohen AE
  TITLE     Video-based pooled screening yields improved far-red genetically 
            encoded voltage indicators.
  JOURNAL   Nat Methods. 2023 Jul;20(7):1082-1094. doi: 
            10.1038/s41592-022-01743-5. Epub 2023 Jan 9.
  PUBMED    36624211
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01743-5"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "1082-1094"
COMMENT     Sequence Label: HT111_Fsyn_FAS(Cre off)_QuasAr6a_Citrine
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3132..3141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3957..3959,3960..4670)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3999..4020)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4260..4279)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4607..4628
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4958..5546
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5011..5031)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5429..5440)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5549..5565)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5550..5566)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6071..6251
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6277..6294)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6283..6507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6553..6981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6640..6659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6850..6871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6990..7010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6995..7324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7175..7310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7237..7256
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7372..7419
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7438..7812
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7942..8063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7979..7998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8033..8052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8112..8128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8112..8128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8125..8147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8136..8152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8160..8166,8167..8184,8185..8190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8205..8226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8343..8360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8514..9102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8594..8613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9273..10064,10065..10133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9896..9915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10134..10238)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaggat ccacaacttc gtatatacct ttctatacga
     3121 agttgtggcg cgccaccatg gtttctatcg cactgcaggc tggttatgac ctcctcggag
     3181 atggtagacc tgaaagtctg tggctcggaa ttggtacact gctgatgctg atcggaacct
     3241 tctacttcct ggttagagcg ggcggagaga ctgacaagga tgcacgggag tactacgcag
     3301 tgacaatcct ggtcagcggt attgccagtg cagcttatct gtcaatgttc tttggtattg
     3361 gcctcacaga ggtgccagta ggtggagaga tcctgaacat ctactacgca agatacgcac
     3421 agtggctgct gactacacca ctgttgctgc tgcacctggc actcctggcc aaagttgata
     3481 gagtgaccat cggcactctc gttggcggcg atgctctgat gattgtcaca ggcctgatag
     3541 ggacactgtc acatactgcc attgccagat actcttggtg cctgttctca actatctgta
     3601 tgatcgtcgt tctgtatgtc ctggccactt ctctgcggtc agccgctaag gagagaggcc
     3661 cagaggttgc ctcaaccttc aacatcctca ccgcactggt tctggtcctg tggactgctt
     3721 accctatcat ctggattatc ggcaccgagg gtgccggagt tgtgggcctg ggaatcgaaa
     3781 ctttgctgtt catggtgctg gacgtcaccg ccaaggtggg cttcggtttt atcctgctca
     3841 ggtcaagaag cattctgcaa gatacagaag cacctgaacc ttcagctgga gctgataaga
     3901 gtagaatcac aagcgaaggc gagtacatcc ccctggatca aatagacata aatgtagtga
     3961 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4021 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4081 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4141 ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg
     4201 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4261 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4321 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4381 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4441 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4501 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4561 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4621 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaaa tcaaggatca
     4681 cctctgaggg cgagtatatc cctctggatc agatcgacat taacgtcggt ggaaaatccc
     4741 gcataacctc tgagggcgag tacatccctc tggatcaaat cgatataaac gtggggggaa
     4801 aaagtcgaat cacttctgag ggcgagtata tacccctcga tcagattgac atcaatgtgg
     4861 ggggcttttg ttatgagaat gaagtgtaag ctagctaaac aacttcgtat atacctttct
     4921 atacgaagtt gtcgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     4981 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5041 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5101 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5161 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5221 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5281 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5341 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5401 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5461 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5521 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     5581 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5641 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5701 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5761 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5821 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     5881 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     5941 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     6001 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     6061 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6121 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6181 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6241 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6301 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6361 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6421 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6481 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6541 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6781 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6841 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6901 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6961 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7081 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7141 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7201 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7261 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7321 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     7381 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     7441 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     7501 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     7561 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     7621 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     7681 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     7741 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     7801 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7861 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7921 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7981 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8041 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8101 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8161 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8221 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8281 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8941 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10261 cctgac
//
