LOCUS       sequence-362513-        8649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G
            promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Domenig SA, Bundschuh N, Lenardic A, Ghosh A, Kim I, Qabrati X, 
            D'Hulst G, Bar-Nur O
  TITLE     CRISPR/Cas9 editing of directly reprogrammed myogenic progenitors 
            restores dystrophin expression in a mouse model of muscular 
            dystrophy.
  JOURNAL   Stem Cell Reports. 2022 Feb 8;17(2):321-336. doi: 
            10.1016/j.stemcr.2021.12.003. Epub 2022 Jan 6.
  PUBMED    34995499
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.12.003"; journalName: "Stem Cell Reports"; 
            date: "2022-02-8- 8"; volume: "17"; issue: "2"; pages: "321-336"
COMMENT     Sequence Label: pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]
FEATURES             Location/Qualifiers
     source          1..8649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..31
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13..31
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        59..285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             286..466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    513..638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1131..1364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1549..1593
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1859..1976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1994..2014
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2020..2038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2056..2074
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2092..2110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2128..2146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2164..2182
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2200..2218
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2236..2254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2299..2323
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2390..2414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2415..2424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3378..3402)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3420..4008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3473..3493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3891..3902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4039..4538
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4074..4097)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4471..4490
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4555..5154
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4555..4574)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5051..5071
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5226..5459
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5531..5652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5568..5587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5622..5641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5692..5827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5847..5866)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5848..5866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5876..5893)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5876..5892)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5885..5907)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6034..6489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6121..6140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6331..6352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6515..6619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6620..6688,6689..7480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6838..6857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7651..8239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8140..8159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8393..8410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8527..8548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8563..8568,8569..8586,8587..8593)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8601..8617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8606..8628
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8625..8641
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8625..8641
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
       61 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
      121 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
      181 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
      241 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
      301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      481 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      541 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      601 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      661 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      721 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      781 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
      841 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
      901 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
      961 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     1021 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1081 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1141 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1201 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1261 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1321 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1381 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1441 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1501 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1561 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1621 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1681 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1741 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1801 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgctagctt
     1861 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1921 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttacta
     1981 gtgattatcg gatcaacttt gtatagaaaa gttgtttact ccctatcagt gatagagaac
     2041 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     2101 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     2161 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     2221 gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt
     2281 gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac
     2341 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa caagtttgta
     2401 caaaaaagca ggctgccacc atggagcttc tatcgccgcc actccgggac atagacttga
     2461 caggccccga cggctctctc tgctcctttg agacagcaga cgacttctat gatgacccgt
     2521 gtttcgactc accagacctg cgcttttttg aggacctgga cccgcgcctg gtgcacatgg
     2581 gagccctcct gaaaccggag gagcacgcac acttccctac tgcggtgcac ccaggcccag
     2641 gcgctcgtga ggatgagcat gtgcgcgcgc ccagcgggca ccaccaggcg ggtcgctgct
     2701 tgctgtgggc ctgcaaggcg tgcaagcgca agaccaccaa cgctgatcgc cgcaaggccg
     2761 ccaccatgcg cgagcgccgc cgcctgagca aagtgaatga ggccttcgag acgctcaagc
     2821 gctgcacgtc cagcaacccg aaccagcggc tacccaaggt ggagatcctg cgcaacgcca
     2881 tccgctacat cgaaggtctg caggctctgc tgcgcgacca ggacgccgcg ccccctggcg
     2941 ccgctgcctt ctacgcacct ggaccgctgc ccccaggccg tggcagcgag cactacagtg
     3001 gcgactcaga tgcatccagc ccgcgctcca actgctctga tggcatgatg gattacagcg
     3061 gccccccaag cggcccccgg cggcagaatg gctacgacac cgcctactac agtgaggcgg
     3121 cgcgcgagtc caggccaggg aagagtgcgg ctgtgtcgag cctcgactgc ctgtccagca
     3181 tagtggagcg catctccaca gacagccccg ctgcgcctgc gctgcttttg gcagatgcac
     3241 caccagagtc gcctccgggt ccgccagagg gggcatccct aagcgacaca gaacagggaa
     3301 cccagacccc gtctcccgac gccgcccctc agtgtcctgc aggctcaaac cccaatgcga
     3361 tttatcaggt gctttgaacc cagctttctt gtacaaagtg gtgataatcg aattccgata
     3421 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3481 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3541 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3601 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3661 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3721 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3781 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3841 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3901 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3961 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcc
     4021 cgcggttcga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4081 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4141 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4201 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4261 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4321 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4381 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     4441 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     4501 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctcacgtggc caccatgacc
     4561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     4981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaggtacc
     5161 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5221 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5281 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5341 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5401 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5461 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5521 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5641 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5821 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     5881 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6001 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6061 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6121 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6181 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6241 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6301 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6361 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6421 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6481 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7141 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8041 gctatgagaa agcgccacgc ttcccgaaga gagaaaggcg gacaggtatc cggtaagcgg
     8101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8401 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8461 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8521 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8581 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8641 ccatgatta
//
