LOCUS       sequence-362517-        9823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing a doxycycline-inducible constitutively 
            active truncated androgen receptor (wild type AR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Hickey TE, Selth LA, Chia KM, Laven-Law G, Milioli HH, Roden D, 
            Jindal S, Hui M, Finlay-Schultz J, Ebrahimie E, Birrell SN, Stelloo 
            S, Iggo R, Alexandrou S, Caldon CE, Abdel-Fatah TM, Ellis IO, Zwart 
            W, Palmieri C, Sartorius CA, Swarbrick A, Lim E, Carroll JS, Tilley 
            WD
  TITLE     The androgen receptor is a tumor suppressor in estrogen 
            receptor-positive breast cancer.
  JOURNAL   Nat Med. 2021 Feb;27(2):310-320. doi: 10.1038/s41591-020-01168-7. 
            Epub 2021 Jan 18.
  PUBMED    33462444
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41591-020-01168-7"; journalName: "Nat Med"; date: 
            "2021-02"; volume: "27"; issue: "2"; pages: "310-320"
COMMENT     Sequence Label: pDRM55 tet-AR(1-707)
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    144..162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     280..304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    328..352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      353..362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2477..2501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2519..2536
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2546..3056
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2975..2993
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3066..3662
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3066..3085)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3562..3582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3663..3716
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3717..4334,4335..4340,4341..4376,4377..4379,4380..4415,
                     4416..4418,4419..4454,4455..4463)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4503..4523)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4921..4932)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5137..5370
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5442..5563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5479..5498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5533..5552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5603..5738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5738..5809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5810..5878,5879..6670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6028..6047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6841..7429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7330..7349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7583..7600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7717..7738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7753..7758,7759..7776,7777..7783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7791..7807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7796..7818
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7815..7831
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7815..7831
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7850..7870
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7852..7870
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7898..8124
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8125..8305
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8352..8477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8970..9203
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9388..9432
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9645..9762
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9816..9823,1..307)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
ORIGIN
        1 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
       61 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      121 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
      181 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      241 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
      301 gatcgcctgg agaattggct agcatcaaca agtttgtaca aaaaagcagg ctgccaccat
      361 ggaagtgcag ttagggctgg gaagggtcta ccctcggccg ccgtccaaga cctaccgagg
      421 agctttccag aatctgttcc agagcgtgcg cgaagtgatc cagaacccgg gccccaggca
      481 cccagaggcc gcgagcgcag cacctcccgg cgccagtttg ctgctgctgc agcagcagca
      541 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcaagagac
      601 tagccccagg cagcagcagc agcagcaggg tgaggatggt tctccccaag cccatcgtag
      661 aggccccaca ggctacctgg tcctggatga ggaacagcaa ccttcacagc cgcagtcggc
      721 cctggagtgc caccccgaga gaggttgcgt cccagagcct ggagccgccg tggccgccag
      781 caaggggctg ccgcagcagc tgccagcacc tccggacgag gatgactcag ctgccccatc
      841 cacgttgtcc ctgctgggcc ccactttccc cggcttaagc agctgctccg ctgaccttaa
      901 agacatcctg agcgaggcca gcaccatgca actccttcag caacagcagc aggaagcagt
      961 atccgaaggc agcagcagcg ggagagcgag ggaggcctcg ggggctccca cttcctccaa
     1021 ggacaattac ttagggggca cttcgaccat ttctgacaac gccaaggagt tgtgtaaggc
     1081 agtgtcggtg tccatgggcc tgggtgtgga ggcgttggag catctgagtc caggggaaca
     1141 gcttcggggg gattgcatgt acgccccact tttgggagtt ccacccgctg tgcgtcccac
     1201 tccttgtgcc ccattggccg aatgcaaagg ttctctgcta gacgacagcg caggcaagag
     1261 cactgaagat actgctgagt attccccttt caagggaggt tacaccaaag ggctagaagg
     1321 cgagagccta ggctgctctg gcagcgctgc agcagggagc tccgggacac ttgaactgcc
     1381 gtctaccctg tctctctaca agtccggagc actggacgag gcagctgcgt accagagtcg
     1441 cgactactac aactttccac tggctctggc cggaccgccg ccccctccgc cgcctcccca
     1501 tccccacgct cgcatcaagc tggagaaccc gctggactac ggcagcgcct gggcggctgc
     1561 ggcggcgcag tgccgctatg gggacctggc gagcctgcat ggcgcgggtg cagcgggacc
     1621 cggttctggg tcaccctcag ccgccgcttc ctcatcctgg cacactctct tcacagccga
     1681 agaaggccag ttgtatggac cgtgtggtgg tggtgggggt ggtggcggcg gcggcggcgg
     1741 cggcggcggc ggcggcggcg gcgaggcggg agctgtagcc ccctacggct acactcggcc
     1801 ccctcagggg ctggcgggcc aggaaagcga cttcaccgca cctgatgtgt ggtaccctgg
     1861 cggcatggtg agcagagtgc cctatcccag tcccacttgt gtcaaaagcg aaatgggccc
     1921 ctggatggat agctactccg gaccttacgg ggacatgcgt ttggagactg ccagggacca
     1981 tgttttgccc attgactatt actttccacc ccagaagacc tgcctgatct gtggagatga
     2041 agcttctggg tgtcactatg gagctctcac atgtggaagc tgcaaggtct tcttcaaaag
     2101 agccgctgaa gggaaacaga agtacctgtg cgccagcaga aatgattgca ctattgataa
     2161 attccgaagg aaaaattgtc catcttgtcg tcttcggaaa tgttatgaag cagggatgac
     2221 tctgggagcc cggaagctga agaaacttgg taatctgaaa ctacaggagg aaggagaggc
     2281 ttccagcacc accagcccca ctgaggagac aacccagaag ctgacagtgt cacacattga
     2341 aggctatgaa tgtcagccca tctttctgaa tgtcctggaa gccattgagc caggtgtagt
     2401 gtgtgctgga cacgacaaca accagcccga ctcctttgca gccttgctct ctagcctcaa
     2461 tgaactggga gagtgaaccc agctttcttg tacaaagtgg tttagtaatg aaccggtcca
     2521 ccaccaccac caccactaag gatccggggt tggggttgcg ccttttccaa ggcagccctg
     2581 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     2641 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     2701 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     2761 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     2821 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     2881 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     2941 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     3001 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat
     3061 tcaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     3121 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     3181 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     3241 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     3301 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     3361 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     3421 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     3481 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     3541 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     3601 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     3661 ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt
     3721 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     3781 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     3841 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     3901 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     3961 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     4021 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     4081 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     4141 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     4201 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     4261 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     4321 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     4381 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     4441 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     4501 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4561 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4621 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4681 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4741 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4801 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4861 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4921 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4981 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5041 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     5101 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5161 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5221 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5281 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5341 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     5401 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     5461 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5521 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5581 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5701 gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat
     5761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7501 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7561 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7621 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7681 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7741 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7801 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7861 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7921 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7981 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     8041 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     8101 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     8161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8281 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8341 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8401 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8461 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8521 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8581 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8641 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8701 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8761 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8821 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8881 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8941 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     9001 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     9061 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     9121 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     9181 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     9241 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9301 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9361 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9421 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9481 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9541 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9601 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9661 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9721 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9781 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tac
//
