LOCUS       sequence-362518-        9814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing a doxycycline-inducible truncated 
            DNA-binding mutant androgen receptor (AR mutant C617Y).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Hickey TE, Selth LA, Chia KM, Laven-Law G, Milioli HH, Roden D, 
            Jindal S, Hui M, Finlay-Schultz J, Ebrahimie E, Birrell SN, Stelloo 
            S, Iggo R, Alexandrou S, Caldon CE, Abdel-Fatah TM, Ellis IO, Zwart 
            W, Palmieri C, Sartorius CA, Swarbrick A, Lim E, Carroll JS, Tilley 
            WD
  TITLE     The androgen receptor is a tumor suppressor in estrogen 
            receptor-positive breast cancer.
  JOURNAL   Nat Med. 2021 Feb;27(2):310-320. doi: 10.1038/s41591-020-01168-7. 
            Epub 2021 Jan 18.
  PUBMED    33462444
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41591-020-01168-7"; journalName: "Nat Med"; date: 
            "2021-02"; volume: "27"; issue: "2"; pages: "310-320"
COMMENT     Sequence Label: pDRM56 tet-AR(1-707)C617Y
FEATURES             Location/Qualifiers
     source          1..9814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    144..162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     280..304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    328..352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      353..362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2468..2492)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2510..2527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2537..3047
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2966..2984
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3057..3653
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3057..3076)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3553..3573
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3654..3707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3708..4325,4326..4331,4332..4367,4368..4370,4371..4406,
                     4407..4409,4410..4445,4446..4454)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4494..4514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4912..4923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5128..5361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5433..5554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5470..5489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5524..5543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5594..5729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5729..5800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5801..5869,5870..6661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6019..6038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6832..7420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7321..7340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7574..7591
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7708..7729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7744..7749,7750..7767,7768..7774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7782..7798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7787..7809
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7806..7822
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7806..7822
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7841..7861
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7843..7861
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7889..8115
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8116..8296
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8343..8468
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8961..9194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9379..9423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9636..9753
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9807..9814,1..307)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
ORIGIN
        1 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
       61 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      121 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
      181 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      241 tcgaggtagg cgggtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
      301 gatcgcctgg agaattggct agcatcaaca agtttgtaca aaaaagcagg ctgccaccat
      361 ggaagtgcag ttagggctgg gaagggtcta ccctcggccg ccgtccaaga cctaccgagg
      421 agctttccag aatctgttcc agagcgtgcg cgaagtgatc cagaacccgg gccccaggca
      481 cccagaggcc gcgagcgcag cacctcccgg cgccagtttg ctgctgctgc agcagcagca
      541 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcaag agactagccc
      601 caggcagcag cagcagcagc agggtgagga tggttctccc caagcccatc gtagaggccc
      661 cacaggctac ctggtcctgg atgaggaaca gcaaccttca cagccgcagt cggccctgga
      721 gtgccacccc gagagaggtt gcgtcccaga gcctggagcc gccgtggccg ccagcaaggg
      781 gctgccgcag cagctgccag cacctccgga cgaggatgac tcagctgccc catccacgtt
      841 gtccctgctg ggccccactt tccccggctt aagcagctgc tccgctgacc ttaaagacat
      901 cctgagcgag gccagcacca tgcaactcct tcagcaacag cagcaggaag cagtatccga
      961 aggcagcagc agcgggagag cgagggaggc ctcgggggct cccacttcct ccaaggacaa
     1021 ttacttaggg ggcacttcga ccatttctga caacgccaag gagttgtgta aggcagtgtc
     1081 ggtgtccatg ggcctgggtg tggaggcgtt ggagcatctg agtccagggg aacagcttcg
     1141 gggggattgc atgtacgccc cacttttggg agttccaccc gctgtgcgtc ccactccttg
     1201 tgccccattg gccgaatgca aaggttctct gctagacgac agcgcaggca agagcactga
     1261 agatactgct gagtattccc ctttcaaggg aggttacacc aaagggctag aaggcgagag
     1321 cctaggctgc tctggcagcg ctgcagcagg gagctccggg acacttgaac tgccgtctac
     1381 cctgtctctc tacaagtccg gagcactgga cgaggcagct gcgtaccaga gtcgcgacta
     1441 ctacaacttt ccactggctc tggccggacc gccgccccct ccgccgcctc cccatcccca
     1501 cgctcgcatc aagctggaga acccgctgga ctacggcagc gcctgggcgg ctgcggcggc
     1561 gcagtgccgc tatggggacc tggcgagcct gcatggcgcg ggtgcagcgg gacccggttc
     1621 tgggtcaccc tcagccgccg cttcctcatc ctggcacact ctcttcacag ccgaagaagg
     1681 ccagttgtat ggaccgtgtg gtggtggtgg gggtggtggc ggcggcggcg gcggcggcgg
     1741 cggcggcggc ggcgaggcgg gagctgtagc cccctacggc tacactcggc cccctcaggg
     1801 gctggcgggc caggaaagcg acttcaccgc acctgatgtg tggtaccctg gcggcatggt
     1861 gagcagagtg ccctatccca gtcccacttg tgtcaaaagc gaaatgggcc cctggatgga
     1921 tagctactcc ggaccttacg gggacatgcg tttggagact gccagggacc atgttttgcc
     1981 cattgactat tactttccac cccagaagac ctgcctgatc tgtggagatg aagcttctgg
     2041 gtgtcactat ggagctctca catgtggaag ctgcaaggtc ttcttcaaaa gagccgctga
     2101 agggaaacag aagtacctgt gcgccagcag aaatgattgc actattgata aattccgaag
     2161 gaaaaattgt ccatcttgtc gtcttcggaa atattatgaa gcagggatga ctctgggagc
     2221 ccggaagctg aagaaacttg gtaatctgaa actacaggag gaaggagagg cttccagcac
     2281 caccagcccc actgaggaga caacccagaa gctgacagtg tcacacattg aaggctatga
     2341 atgtcagccc atctttctga atgtcctgga agccattgag ccaggtgtag tgtgtgctgg
     2401 acacgacaac aaccagcccg actcctttgc agccttgctc tctagcctca atgaactggg
     2461 agagtgaacc cagctttctt gtacaaagtg gtttagtaat gaaccggtcc accaccacca
     2521 ccaccactaa ggatccgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     2581 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     2641 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     2701 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     2761 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     2821 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     2881 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     2941 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     3001 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagcaa ttcaccatga
     3061 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     3121 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     3181 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     3241 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     3301 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     3361 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     3421 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     3481 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     3541 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     3601 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgaaggta
     3661 gaggttctct cctcacttgt ggtgatgttg aagaaaaccc tggtccaatg tctagactgg
     3721 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     3781 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     3841 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     3901 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     3961 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     4021 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     4081 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     4141 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     4201 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     4261 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     4321 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     4381 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     4441 tgctccccgg gtaactaagt aaggatcgat ccaagatatc gtattcttaa ctatgttgct
     4501 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4561 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4621 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4681 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4741 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4801 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4861 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4921 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4981 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtttcgcct
     5041 cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct gtagatctta
     5101 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5161 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5221 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5281 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5341 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5401 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5461 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5521 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5581 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5641 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5701 gaagtagtga ggaggctttt ttggaggcct ttcaaatatg tatccgctca tgagacaata
     5761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6481 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6661 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7501 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7561 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7621 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7681 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7741 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7801 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     7861 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     7921 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     7981 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     8041 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     8101 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8281 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8341 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8401 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8461 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8521 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8581 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8641 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8701 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8761 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     8821 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8881 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8941 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9001 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9061 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9121 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9181 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9241 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9301 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9361 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9421 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9481 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9541 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacaattc
     9601 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9661 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9721 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     9781 ccactttggc cgcgaatcga tatgtcgagt ttac
//
