LOCUS       sequence-362519-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, 
            and the blasticidin resistance gene joined by P2A linkers..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Mustafa EH, Laven-Law G, Kikhtyak Z, Nguyen V, Ali S, Pace AA, Iggo 
            R, Kebede A, Noll B, Wang S, Winter JM, Dwyer AR, Tilley WD, Hickey 
            TE
  TITLE     Selective inhibition of CDK9 in triple negative breast cancer.
  JOURNAL   Oncogene. 2023 Nov 24. doi: 10.1038/s41388-023-02892-3.
  PUBMED    38001268
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2023 Nov 24. doi: 10.1038/s41388-023-02892-3."
COMMENT     Sequence Label: pDRM166 LKB (Luciferase mKate2 Blast)
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      577..586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             583..2232
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(649..667)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             2248..2304
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      2311..2320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2323..2343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3061..3117
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3118..3513
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3520..4108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3573..3593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3991..4002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4195..4428
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4500..4621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4537..4556)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4591..4610
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4661..4796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4816..4835)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4817..4835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4845..4862)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4845..4861)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4854..4876)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5003..5458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5090..5109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5300..5321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5484..5588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5589..5657,5658..6449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5807..5826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6620..7208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7109..7128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7362..7379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7496..7517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7532..7537,7538..7555,7556..7562)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7570..7586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7575..7597
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7594..7610
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7594..7610
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7629..7649
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7631..7649
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7677..7903
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7904..8084
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8131..8256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8749..8982
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9167..9211
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9478..9595
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atatcgagat cgattagtcc aatttgttaa agacaggata tcagtggtcc aggctctagt
       61 tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga tagaataaaa
      121 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
      181 gctaggatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
      241 gtaagcagtt cctgccccgg ctcagggcca agaacagttg gaacagcaga atatgggcca
      301 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
      361 cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
      421 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
      481 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
      541 gatcgatgaa ttcgagctcg gtacccgggg atccaagcca ccatggaaga cgccaaaaac
      601 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
      661 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
      721 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
      781 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
      841 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
      901 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
      961 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
     1021 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     1081 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
     1141 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
     1201 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     1261 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     1321 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     1381 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
     1441 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     1501 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     1561 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     1621 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     1681 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     1741 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     1801 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     1861 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     1921 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     1981 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     2041 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     2101 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     2161 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     2221 aagatcgccg tggctagcgg tagtggtgca accaatttta gtctactcaa acaagcaggt
     2281 gatgtcgaag aaaatccagg gcccaccggt gccaccatgg cccctaaaaa gaaacgcaag
     2341 gtcggatccg gtggttctgt gtccgagttg ataaaggaga atatgcacat gaagctgtat
     2401 atggagggca ccgtcaataa ccatcacttc aagtgtacgt cagaaggcga ggggaagccc
     2461 tacgaaggaa cgcagaccat gcggatcaaa gctgttgaag gcggcccact cccattcgcg
     2521 ttcgacatct tggcaacgtc ttttatgtac ggctcaaaaa cctttataaa tcacactcag
     2581 ggaattccgg atttcttcaa gcaatctttc ccggaagggt tcacttggga acgcgtgact
     2641 acttatgaag atggaggtgt tttgacagct acgcaagata cgtctcttca ggacgggtgc
     2701 ctcatataca atgtcaaaat ccggggagta aatttcccca gcaatggtcc ggtgatgcag
     2761 aaaaaaacac ttggatggga agcaagcacg gagacacttt atcccgcgga cggtgggctg
     2821 gagggcagag ctgatatggc gcttaagttg gtaggagggg gacacttgat ctgcaatctc
     2881 aaaactacat acagatccaa gaagcccgca aaaaacctca aaatgccagg tgtgtactat
     2941 gtggatagga ggctggagcg aataaaagaa gcagataagg agacctatgt tgaacaacac
     3001 gaggttgcag ttgcccgcta ttgtgacttg ccaagcaagc ttggccacag aggatccggc
     3061 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccggcc
     3121 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     3181 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     3241 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     3301 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     3361 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     3421 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     3481 ttgctgccct ctggttatgt gtgggagggc taagtcgaca atcaacctct ggattacaaa
     3541 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3601 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3661 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3721 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3781 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3841 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3901 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     3961 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4021 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4081 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa
     4141 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4201 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4261 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4321 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4381 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     4441 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4621 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4681 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4741 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4801 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4861 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4921 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4981 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5041 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5101 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5161 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5221 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5281 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5341 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5401 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5461 gtggcacttt tcggggaaat gtgcgcggaa tccctatttg tttatttttc taaatacatt
     5521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7321 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7381 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7441 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7501 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7561 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7621 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7681 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7741 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7801 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7861 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     7921 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7981 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8041 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8101 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8161 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8221 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8281 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8341 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8401 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8461 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8521 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8581 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8641 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8701 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8761 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8821 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8881 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8941 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9001 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9061 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9121 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9181 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9241 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9301 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9361 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     9421 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     9481 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9541 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     9601 tctcgatatc ctcgagg
//
