LOCUS       sequence-36255-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Milenkovic L, Scott MP, Rohatgi R
  TITLE     Lateral transport of Smoothened from the plasma membrane to the 
            membrane of the cilium.
  JOURNAL   J Cell Biol. 2009 Nov 2. 187(3):365-74.
  PUBMED    19948480
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Nov 2. 187(3):365-74."
COMMENT     Sequence Label: pCS2-SNAP-Smo
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             178..723
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for mammalian expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gaccatcgat tcgaattcac catggccgct ggccgccccg tgcgtgggcc cgagctggcg
       61 ccccggaggc tgctgcagtt gctgctgctg gtactgctgg ggggcccggg ccggggggcg
      121 gccttgagcg ggaacgtgac cgggcctggg cctcacagcg ccagcgggag ctcgagcatg
      181 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
      241 tctgggtgcg aacagggcct gcacgagatc aagctgctgg gcaaaggaac atctgccgcc
      301 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
      361 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
      421 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
      481 ctgctgaaag tggtgaagtt cggagaggtc atcagctacc agcagctggc cgccctggcc
      541 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
      601 ctgatcccct gccaccgggt ggtgtctagc tctggcgccg tggggggcta cgagggcggg
      661 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
      721 ggcagctcga ggaggaacgt gccggtgacc agccctccgc cgccgctgct gagccactgc
      781 ggccgggccg cccactgcga gcctttgcgc tacaacgtgt gcctgggctc ggcgctgccc
      841 tacggagcca ccaccacgct gctggctggg gactcggact cncagganga ancgcacggc
      901 aagctcgtgc tctggtccgg cctccgnaat atccc
//
