LOCUS       sequence-362554-        8747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter construct with single core HS4 insulator, 
            upstream TRE (Tetracycline Response Element) recruitment site, 
            phiC31 attB for integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: 
            pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..8747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..85
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             351..1154
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(414..433)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1024..1043
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1186..1203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1192..1416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1489..1507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1525..1543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1561..1579
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1596..1614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1632..1650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          1895..2833
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2790..2810
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        2866..2884
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2975..3352
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3371..3380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3377..3379,3380..3382,3383..4093)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3422..3443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3683..3702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4030..4051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4144..4163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4145..4163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4437..4558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4876..5138
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             5152..5529
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      5542..5551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5548..6261
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(5693..5711)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5961..5980
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6377..6458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6501..6564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6565..6633,6634..7425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6783..6802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7596..8184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8085..8104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    8368..8502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8605..8726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
       61 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      121 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      181 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      241 cgtcagcggg ttctcgacgg tcacggcggg catgtcgacg gtatcgataa gcttacgcgt
      301 gctagcgcat gcagatctac tagtcggccg gataaagata tcaactcgag atgggatcgg
      361 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
      901 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
      961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1141 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     1201 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1261 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1321 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     1381 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1441 gggggctagc aaagcctagg aagacgtacg tatacgcgtc gagtttactc cctatcagtg
     1501 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     1561 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     1621 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     1681 aatgcatgag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1741 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     1801 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     1861 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     1921 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     1981 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2041 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2101 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2161 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2221 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2281 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2341 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2401 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2461 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2521 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2581 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2641 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2701 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     2761 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     2821 tcttccattt caggtgtcgt gagctagcga ttacgccaag ctcgaaatta accctcacta
     2881 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagcccgg gcggatcccc
     2941 cgggctgcag gaattcgata tcaagcttgc caccatgcca gagccagcga agtctgctcc
     3001 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     3061 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     3121 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     3181 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     3241 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     3301 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccccg
     3361 ggtaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3421 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3481 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3541 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta
     3601 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3661 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3721 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3781 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3841 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3901 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3961 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     4021 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4081 cgagctgtac aagtaggtcg acctcgaggg ggggcccggt accaggtaag tgtacccaat
     4141 tcgccctata gtgagtcgta ttacaattca ctcgatcgcc cttcccaaca gttgcgcagc
     4201 ctgaatggcg aatggagatc caatttttaa gtgtataatg tgttaaacta ctgattctaa
     4261 ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca
     4321 gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4381 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     4561 gcgtaaattg taagcgtctc aaagtaacaa aacttttatg agggacagcc cccccccaaa
     4621 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
     4681 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
     4741 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
     4801 cctgcagaca cctgggggga tacggggaaa aggcctccac ggccactagt attatgccct
     4861 agggacgtcc atatgaatgt agtcttatgc aatacacttg tagtcttgca acatggtaac
     4921 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     4981 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acaggtctga catggattgg
     5041 acgaaccact gaattgcgca ttgcagagat aattgtattt aagtgcctag ctcgatacaa
     5101 taaacgccat ttgaccattc accacattgg tgtgcaccct gcagatctag aatgccagag
     5161 ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag
     5221 aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac
     5281 aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg
     5341 aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat
     5401 tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg
     5461 cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc
     5521 agcgctaagg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     5581 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     5641 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     5701 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     5761 tacggctcca aggcctacgt gaagcacccc gccgacattc ccgactactt gaagctgtcc
     5821 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     5881 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     5941 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     6001 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     6061 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     6121 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     6181 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     6241 gacgagctgt acaagtctta agcgactcta gatcataatc agccatacca catttgtaga
     6301 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     6361 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6421 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6481 actcatcaat gtatctcata tgtgtatccg ctcatgagac aataaccctg ataaatgctt
     6541 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6601 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6661 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6721 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6781 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6841 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6901 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6961 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7021 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7081 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7141 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7201 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7261 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7321 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7381 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7441 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7501 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7561 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7621 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7681 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7741 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7801 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7861 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7921 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7981 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8041 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8101 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8161 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8221 ttttgctggc cttttgctca catgttcttt cctgcggtac cgatcataat cagccatacc
     8281 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     8341 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     8401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8461 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcataatcag ccataccaca
     8521 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8581 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8701 ttgtccaaac tcatcaatgt atcttatcat gtctgctcga ggtcgac
//
